Abl2 (NM_001136104) Mouse Tagged ORF Clone

CAT#: MR225599

Abl2 (Myc-DDK-tagged) - Mouse v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (Abl2), transcript variant 1

Product Images

Circular map for MR225599

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abl2
• Synonyms: AA536808; Abll; Arg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225599 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCAGGTGGGCCGCGTCGGGGAGGCTCCGGGGCTCCAGCAGCCGCAGCCGCGCGGGATCCGGG
  GCAGCAGCGCAGCCAGGCCCTCGGGCCGCAGGCGGGACCCGGCGGGGCGTACCGCAGACGCCGGCTTCAA
  CGTCTTCACCCAGCACGATCACTTTGCCAGCTGTGTGGAGGATGGATTTGAGGGAGACAAGACTGGAGGC
  AGTAGTCCAGAAGTTTTGCACCGCCCGTTTGGCTGTGATGCTGAATCTCAGGCACTGAATGAAGCGATCA
  GGTGGAGCTCCAAGGAGAACTTGCTGGGAGCCACTGAGAGTGACCCTAATCTCTTTGTTGCACTTTATGA
  CTTTGTGGCAAGTGGTGATAACACACTCAGTATCACTAAAGGTGAAAAGCTGCGAGTCCTTGGTTATAAC
  CAGAATGGCGAGTGGAGTGAAGTTCGCTCCAAGAATGGACAGGGTTGGGTGCCAAGCAACTACATCACTC
  CAGTTAATAGCCTGGAGAAACATTCCTGGTACCACGGACCTGTATCCCGCAGCGCAGCAGAGTATCTCCT
  CAGCAGCCTAATCAATGGCAGCTTCCTGGTTCGAGAGAGTGAGAGCAGCCCTGGGCAGCTGTCCATCTCT
  CTCAGGTATGAGGGACGTGTGTATCACTACAGGATCAATACCACCACAGACAGCAAGGTGTACGTGACAG
  CTGAGAGCCGCTTTAGCACCTTGGCAGAGCTTGTTCACCACCACTCCACAGTTGCTGATGGGCTAGTGAC
  CACGCTGCACTACCCAGCACCGAAGTGCAACAAGCCAACCGTCTATGGCGTGTCTCCTATCCATGACAAG
  TGGGAAATGGAACGGACAGATATCACCATGAAGCACAAACTTGGGGGCGGTCAGTACGGAGAGGTTTACG
  TTGGCGTCTGGAAGAAGTACAGCCTTACAGTGGCTGTGAAAACACTGAAGGAAGACACCATGGAGGTGGA
  GGAGTTCCTGAAGGAAGCTGCAGTGATGAAGGAGATCAAGCATCCTAACTTAGTACAACTGCTAGGTGTG
  TGTACCCTGGAGCCACCGTTTTACATTGTGACTGAATACATGCCGTATGGGAACCTGCTTGACTATCTCC
  GGGAGTGCAGCCGAGAGGAGGTGACCGCCGTCGTGTTACTTTACATGGCCACCCAGATCTCCTCTGCCAT
  GGAGTACTTGGAGAAGAAGAACTTCATCCATAGAGATCTTGCTGCCCGGAACTGCCTAGTGGGCGAGAAC
  CATGTGGTGAAAGTGGCCGACTTTGGTTTAAGTAGACTGATGACTGGAGATACCTACACTGCTCATGCTG
  GAGCCAAATTTCCTATTAAATGGACAGCACCCGAGAGTCTGGCCTACAATACCTTTTCAATTAAATCTGA
  CGTTTGGGCGTTTGGAGTACTGTTGTGGGAAATTGCTACATATGGAATGTCACCATATCCAGGTATTGAC
  CTATCTCAAGTCTATGACCTACTGGAAAAAGGATATCGAATGGAACAGCCTGAGGGATGCCCCCCTAAAG
  TGTATGAACTTATGAGAGCATGCTGGAAGTGGAGCCCTGCTGACAGGCCCTCTTTTGCTGAAACCCATCA
  AGCTTTTGAAACAATGTTCCATGACTCCAGCATCTCTGAAGAGGTAGCTGAGGAGCTTGGGAGAACGGCC
  TCCTCCTCCTCTGTGGTTCCATACCTGCCTCGGTTACCGCTGCTTCCTTCCAAGACGAGGACCCTGAGGA
  AGCAGGGGGAGAACAAAGAGAACCTAGATGGGGGCCTTGATGCCGCCGAGAGCCTGGCCTCCAGCTCAGC
  ACCAGCAGGGTTCATTAGAAGCACACAGGCCTCCAGTGGGTCCCCAGCTCTGCCTCGAAAGCAAAGAGAT
  AAGTCGCCCAGCAGCCTCTTAGAAGATGCCAAAGAGACATGCTTTACCAGGGATAGGAAGGGGGGCTTCT
  TCAGCTCCTTCATGAAAAAGAGGAACGCCCCCACGCCCCCGAAGCGCAGCAGCTCCTTTCGAGAAATGGA
  GAATCAGCCCCACAAGAAATATGAACTCACGGGGCTTCCAGAGCAGGATAGGATGGCAATGACCCTTCCC
  AGGAACTGCCAGAGGTCCAAACTCCAGCTGGAAAGGACAGTGTCCACCTCTTCTCAACCAGAAGAGAATG
  TGGACAGGGCTAATGACATGCTTCCAAAAAAATCTGAGGAAGGTGCTGCTCCAGCCAGGGAGAGACCAAA
  AGCCAAACTATTGCCCAGAGGAGCCACAGCTCTTCCCTTGAGAGCCCCTGATCCAGCCATCACAGAGAGT
  GACTCTCCGGGGGTAGGGGTGGCTGGAGTGGCAGCTGCCCCCAAAGGCAAGGAAAGGAATGGTGGGACGC
  GACTTGGAGTCGCTGGAGTCCCCGAGGATGGAGAGCAGCTAGGCTGGTCTTCTCCAGCCAAGGCTGTAGC
  TGTCCTCCCAACCACTCACAACCACAAAGTGCCAGTCCTTATCTCTCCCACTCTGAAACACACTCCAGCT
  GATGTGCAGCTCATTGGCACAGACTCTCAGGGGAACAAATTCAAGCTCTTATCTGAGCATCAGGTCACAT
  CCTCTGGAGACAAGGACCGACCCCGTCGGGTAAAACCAAAGTGTGCCCCACCCCCCCCACCAGTGATGAG
  ACTACTGCAGCATCCGTCCACATGCTCAGACCCCGAGGAAGAGCCGACTGCCCCACCTGCAGGACAGCAC
  ACTCCAGAGACCCAGGAGGGAGGAAAAAAGGCAGCTCCAGGCCCAATGCCCAGTAGTGGGAAACCTGGGA
  GGCCAGTCATGCCTCCACCTCAAGTGCCTTTGCCCACATCTTCCATCTCACCGGCCAAAATGGCCAATGG
  CACAGCAGGTACTAAGGTGGCCCTGAGGAAAACCAAACAGGCAGCTGAGAAAATCTCAGCTGACAAAATC
  AGCAAAGAGGCCCTGCTGGAGTGTGCCGACCTACTGTCCAGTGCAATCACGGAACCTGTGCCCAACAGCC
  AACTGGTGGACACTGGGCACCAGCTGCTCGACTACTGCTCAGGGTATGTGGACAGCATCCCTCAGACTCG
  CAACAAGTTTGCCTTCCGAGAGGCTGTGAGCAAACTGGAACTTAGCTTACAGGAGCTGCAGGTGTCTTCC
  ACAGCTGCTGGTGTGCCTGGGACAAACCCCGTCCTTAATAACTTATTGTCGTGTGTACAGGAAATTAGCG
  ATGTGGTGCAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQQVGRVGEAPGLQQPQPRGIRGSSAARPSGRRRDPAGRTADAGFNVFTQHDHFASCVEDGFEGDKTGG
  SSPEVLHRPFGCDAESQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYN
  QNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSIS
  LRYEGRVYHYRINTTTDSKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDK
  WEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGV
  CTLEPPFYIVTEYMPYGNLLDYLRECSREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGEN
  HVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGID
  LSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRTA
  SSSSVVPYLPRLPLLPSKTRTLRKQGENKENLDGGLDAAESLASSSAPAGFIRSTQASSGSPALPRKQRD
  KSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELTGLPEQDRMAMTLP
  RNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEEGAAPARERPKAKLLPRGATALPLRAPDPAITES
  DSPGVGVAGVAAAPKGKERNGGTRLGVAGVPEDGEQLGWSSPAKAVAVLPTTHNHKVPVLISPTLKHTPA
  DVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSTCSDPEEEPTAPPAGQH
  TPETQEGGKKAAPGPMPSSGKPGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKI
  SKEALLECADLLSSAITEPVPNSQLVDTGHQLLDYCSGYVDSIPQTRNKFAFREAVSKLELSLQELQVSS
  TAAGVPGTNPVLNNLLSCVQEISDVVQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136104
• ORF Size: 3237 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136104.1, NP_001129576.1
• RefSeq Size: 10402 bp
• RefSeq ORF: 3237 bp
• Locus ID: 11352
• Cytogenetics: 1 67.71 cM
• MW: 117.8 kDa