Kcnk10 (NM_029911) Mouse Tagged ORF Clone

CAT#: MR221222

Kcnk10 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 10 (Kcnk10)

Product Images

Circular map for MR221222

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnk10
• Synonyms: 1700024D23Rik; 3010005K24Rik; Trek2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221222 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTTTCCTACATAGGTTACTTCTTTCTTCCTCCTTTGGTGGCCGTGCCTGCAGCAGCCCCACCAG
  TGTGCCAGCCCAAGAGTGCCACTAACGGGCATCACCCTGTTCCTCGACTCTCCATTTCCTCTCGAGCCAC
  GGTGGTAGCCAGGATGGAAGGCGCCTCCCAGGGAGGCCTGCAAACAGTTATGAAGTGGAAAACAGTGGTT
  GCCATCTTCGTGGTGGTCGTGGTCTACCTCGTCACTGGTGGCCTTGTCTTCCGGGCCTTGGAACAGCCCT
  TCGAGAGCAGTCAGAAGAACACAATCGCCTTGGAGAAGGCAGAATTCTTGAGAGATCACATCTGTGTGAG
  TCCCCAGGAACTAGAGACACTGATTCAGCATGCACTCGATGCTGATAACGCGGGAGTCAGCCCAGTAGGA
  AACTCTTCCAACAGCAGCAGTCACTGGGACCTTGGAAGTGCCTTCTTCTTTGCTGGGACAGTCATCACAA
  CCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAATCTTTTGTATTTTATATGCCATCTT
  TGGGATCCCGCTTTTTGGTTTCTTATTGGCTGGAATTGGAGACCAACTTGGAACCATCTTTGGCAAAAGC
  ATTGCAAGAGTGGAAAAGGTTTTTCGAAAGAAGCAAGTGAGCCAGACCAAGATCCGGGTCATCTCCACGA
  TTCTCTTCATCTTGGCTGGCTGCATCGTGTTTGTGACCATCCCTGCTGTCATCTTTAAATACATCGAGGG
  TTGGACCGCCTTGGAGTCCATCTACTTTGTGGTAGTCACGCTGACCACAGTAGGCTTTGGTGATTTTGTG
  GCAGGGGGAAATGCTGGCATCAATTACCGAGAGTGGTACAAGCCGCTGGTGTGGTTTTGGATCCTTGTTG
  GCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATCGGAGACTGGCTGCGGGTTTTATCCAAAAAGACAAA
  AGAAGAGGTTGGTGAGATCAAAGCCCACGCAGCTGAGTGGAAGGCTAATGTCACTGCTGAGTTCCGGGAG
  ACGAGAAGGCGGCTCAGCGTTGAGATCCATGACAAGCTGCAACGGGCAGCCACTATCCGAAGTATGGAGC
  GCCGGAGGCTAGGCTTGGACCAGAGGGCCCACTCACTGGACATGCTATCCCCTGAGAAGCGTTCTGTCTT
  TGCAGCCCTGGACACAGGCCGTTTCAAGGCCTCATCGCAGGAGAGTATCAACAACAGACCCAACAACCTA
  CGCCTTAAGGGGCCAGAACAGCTTACCAAACATGGGCAGGGCGCTTCTGAGGACAACATCATCAACAAGT
  TTGGGTCCACCTCCAAACTCACAAAGAGGAAAAACAAAGATCTCAAAAAGACCTTACCCGAGGATGTTCA
  GAAAATCTACAAAACCTTCCGGAATTACTCTCTGGATGAAGAGAAGAAAGAGGACGAGACAGAAAAGATG
  TGTAACTCCGACAACTCCAGCACAGCCATGCTGACAGAGTGTATACAGCAGCAAGCTGAGATGGAGAACG
  GAATGGTACCCACGGACACCAAAGACCAGGGGCTGGAGAACAATTCTTTACTTGAAGACAGAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221222 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYFSYIGYFFLPPLVAVPAAAPPVCQPKSATNGHHPVPRLSISSRATVVARMEGASQGGLQTVMKWKTVV
  AIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHICVSPQELETLIQHALDADNAGVSPVG
  NSSNSSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKS
  IARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFV
  AGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRE
  TRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNL
  RLKGPEQLTKHGQGASEDNIINKFGSTSKLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEDETEKM
  CNSDNSSTAMLTECIQQQAEMENGMVPTDTKDQGLENNSLLEDRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029911
• ORF Size: 1608 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029911.2, NM_029911.3, NM_029911.4, NM_029911.5, NP_084187.2
• RefSeq Size: 2533 bp
• RefSeq ORF: 1608 bp
• Locus ID: 72258
• Cytogenetics: 12 E
• MW: 59.4 kDa