Cdc23 (NM_178347) Mouse Tagged ORF Clone

CAT#: MR215901

Cdc23 (Myc-DDK-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (Cdc23)

Product Images

Circular map for MR215901

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc23
• Synonyms: 6030435O18; D18Ertd243e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215901 representing NM_178347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGAATTCTTCGGTAGTCTCGGTGGCAGCGGCCGCCACGGCGGTGCCGGGCGTATCCACTGTCG
  CGGATTTTTCAGACTTGCAGGAAATTAAAAAGCAGCTGCTGCTCATCGCGGGCCTCACTCGGGAGCGGGG
  CCTGCTGCATAGCAGCAAATGGTCTGCGGAGTTGGCCTTCTCTCTGCCCGCCTTGCCTTTGTCGGAGCTG
  CAGCCGCCGCCGCCTCTGACAGAGGAGGACGCGCAGGATGTGGATGCTTACACCCTGGCCAAGGCCTACT
  TTGATGTTAAAGAGTATGACCGGGCGGCACATTTCCTTCACGGCTGCAATAGCAAGAAAGCCTATTTCCT
  GTACATGTATTCCAGATACCTGTCTGGAGAGAAGAAGAAGGATGACGAAACAGTTGATAGCCTAGGACCC
  CTAGAGAAGGGACAAGTCAAAAACGAGGCTCTCAGAGAACTGAGAGTGGAACTCAGCCGGAAGCACCAGG
  CTCGGGGACTTGATGGATTCGGCCTTTACCTGTATGGGGTGGTGCTTCGGAAACTGGACTTAGTGAAAGA
  GGCCATTGATGTGTTTGTGGAGGCTACTCATGTTTTGCCTTTGCACTGGGGAGCCTGGTTAGAGCTGTGT
  AACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCTGACACCTGGATGAAAGAGTTCTTCT
  TGGCTCACATATACACAGAACTGCAGCTGATAGAGGAGGCCCTGCAGAAGTATCAGCATCTCATTGATGT
  GGGCTTTTCCAAGAGCTCGTACATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATCGAC
  AAAGCCCTTTCTATTTTCAATGAGCTGAGGAAACAAGATCCTTACAGGATTGAAAATATGGACACATTCT
  CCAACCTTCTCTATGTCAGGAGCATGAAGTCTGAATTGAGTTACCTGGCGCATAACCTCTGTGAAATTGA
  TAAATACCGAGTAGAAACATGCTGTGTAATTGGCAATTATTACAGCTTACGCTCCCAGCATGAGAAAGCA
  GCTTTGTATTTCCAGAGAGCCCTAAAGTTGAATCCTAGGTACCTTGGGGCCTGGACACTGATGGGGCACG
  AGTACATGGAGATGAAGAACACGTCTGCAGCTATTCAGGCTTACCGACATGCCATTGAGGTCAACAAGAG
  AGACTACAGAGCTTGGTATGGCCTTGGGCAGACTTACGAAATCCTTAAAATGCCATTTTATTGCCTTTAT
  TATTATAGACGGGCCCACCAGCTTCGTCCCAATGATTCTCGCATGCTGGTTGCCTTAGGAGAATGTTATG
  AGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCATATGCCGTGGGAGACGTGGAGAA
  AATGGCTCTTGTGAAGCTGGCCAAGCTTCATGAACAGTTGACTGAGTCAGAGCAGGCTGCCCAGTGTTAC
  ATCAAATACATCCAAGATATCTATTCCTGTGGGGAGACCGTGGAGCACTTGGAGGAGAGCACAGCCTTCC
  GATACCTGGCCCAGTACTATTTTAAGTGCAAGCTGTGGGATGAAGCTTCAACTTGTGCCCAAAAATGTTG
  TGCATTCAATGATACCCGGGAAGAAGGTAAGGCCTTGCTCCGCCAGATCCTACAGCTGAGGAACCAAGGG
  GAGACACCCACCTCTGACACGCCGGGTACCTTTTTCCTCCCTGCCTCACTGTCTGCAAACAACACTCCTA
  CACGCAGAGTTTCTCCACTCAACCTGTCTTCAGTCACACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215901 representing NM_178347
  Red=Cloning site Green=Tags(s)
  
  MAANSSVVSVAAAATAVPGVSTVADFSDLQEIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLSEL
  QPPPPLTEEDAQDVDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGP
  LEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELC
  NLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQHLIDVGFSKSSYIVSQIAVAYHNIRDID
  KALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKA
  ALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLY
  YYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCY
  IKYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQG
  ETPTSDTPGTFFLPASLSANNTPTRRVSPLNLSSVTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178347
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178347.4, NP_848124.1
• RefSeq Size: 4134 bp
• RefSeq ORF: 1794 bp
• Locus ID: 52563
• UniProt ID: Q8BGZ4
• Cytogenetics: 18 18.69 cM
• MW: 69 kDa
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]