Foxl1 (NM_008024) Mouse Tagged ORF Clone

CAT#: MR215803

Foxl1 (Myc-DDK-tagged) - Mouse forkhead box L1 (Foxl1)

Product Images

Circular map for MR215803

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxl1
• Synonyms: fkh-6; Fkh6; FREAC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215803 representing NM_008024
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCACCTCTTCAGTCCCCCGCTGGCCGCTCTGGCAGCCTCGCCGCTGTTGTACGTGTACAGCCCCG
  AGAGGCCTGGGCTGCCCCTGGCCTTCGCCCCTGCGGCTGCACTGGCCGGCCCGGGCCGCGTCGAGCCCCC
  GCAGAAGCCGCCCTACAGCTACATCGCTCTTATAGCCATGGCGATCCAGGATGCTCCCGAGCAGAGGGTC
  ACACTGAACGGCATCTACCAGTTCATCATGGATCGCTTCCCGTTCTACCACGACAACCGCCAAGGCTGGC
  AGAACAGCATCCGCCACAACCTGTCGCTCAACGAGTGCTTCGTGAAGGTGCCACGTGAGAAGGGGCGGCC
  GGGCAAGGGCAGCTACTGGACCCTGGACCCCCGCTGCTTGGACATGTTTGAGAACGGCAATTATCGGCGC
  AGGAAGAGGAAGCCCAAGCCCGCCGCGGGTTCCCCGGAGGCCAAGCGCACCCGGGTCGAGCCGCCGGAGT
  CCGAGGTGGGCTGCGATGTAGGGTCCCCGGACCTGGCCACTGCACTCCCGACGCGCGCGCCGGATCGCTC
  CCAATCCCCGGCAGTAGGCACCGCGCGGCCCGCGCTCCTTCCCTGGCCCGGCCCTGAGCCCCGGGACCCC
  GACGCGGACCTCACAGTCCAGGGCGCAGGGGCAGTGGCAAGCGGCCAGCTCCAGCGTCCGGCACACCACC
  TGGGGTCCCCTCTGTGCCCCGCCCCCAGCGGCTCCCCAAAAGGTTCCAAGTCAAAGAGTTTCAGCATCGA
  CAGCATCTTGGCCGTGAGACCGACGCCAGCCTCAGGGGCCGAAGCACCGGGGATCCCTAAGCCCGTGCCT
  GGCGCGCTGGGCTCCTCTCTGCTGGCTGCCTCCTCCGGCCTAGCGCCTCCCTTCAACGCTTCGCTGGTCT
  TCGACGCGCACGTCCAGGGCGGCTTTTCCCAGCTGGGGATTCCCTTCCTTTCTTATTTTCCTCTGCAGGT
  TCCCGAGGCCACAGTGCTTCGTTTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215803 representing NM_008024
  Red=Cloning site Green=Tags(s)
  
  MSHLFSPPLAALAASPLLYVYSPERPGLPLAFAPAAALAGPGRVEPPQKPPYSYIALIAMAIQDAPEQRV
  TLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNECFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRR
  RKRKPKPAAGSPEAKRTRVEPPESEVGCDVGSPDLATALPTRAPDRSQSPAVGTARPALLPWPGPEPRDP
  DADLTVQGAGAVASGQLQRPAHHLGSPLCPAPSGSPKGSKSKSFSIDSILAVRPTPASGAEAPGIPKPVP
  GALGSSLLAASSGLAPPFNASLVFDAHVQGGFSQLGIPFLSYFPLQVPEATVLRFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008024
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008024.2, NP_032050.2
• RefSeq Size: 2960 bp
• RefSeq ORF: 1011 bp
• Locus ID: 14241
• UniProt ID: Q64731
• Cytogenetics: 8 70.34 cM
• MW: 36.2 kDa
• Gene Summary: Transcription factor required for proper proliferation and differentiation in the gastrointestinal epithelium. Target gene of the hedgehog (Hh) signaling pathway via GLI2 AND GLI3 transcription factors.[UniProtKB/Swiss-Prot Function]