Lars (NM_134137) Mouse Tagged ORF Clone

CAT#: MR211761

Lars (Myc-DDK-tagged) - Mouse leucyl-tRNA synthetase (Lars)

Product Images

Circular map for MR211761

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lars
• Synonyms: 2310045K21Rik; 3110009L02Rik; AW536573; mKIAA1352
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211761 representing NM_134137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAAGAAAAGGAACTGCTAAAGTAGACTTCTTGAAGGAGATCGAGAAAGAGGCCCAGCAGAAGT
  GGGAAGCTGAGAAAGTGTTTGAAGTCAGTGCCTCCCGGCTGGAGAAGCAGAAGCAGAGCAGCAAGGGCAA
  ATACTTTGTCACCTTCCCATATCCATACATGAATGGACGCCTTCATTTGGGACACACGTTTTCCTTATCC
  AAGTGTGAGTTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTTCCCTTTGGGTTGCACT
  GTACTGGTATGCCTATTAAGGCATGCGCTGACAAGCTGAAAAGAGAAATAGAGCTGTATGGCTGCCCTCC
  TGATTTTCCCGAGGAAGAAGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAGTGAGGGAT
  AAAGCCAAAGGGAAAAAGAGCAAAGCTGCCGCCAAAGCAGGATCTTCTAAGTACCAGTGGGACATTATGA
  AATCCCTTGGGCTCTCTGATGACGACATAGTGAAGTTTTCTGAAGCAGAGCACTGGCTTGATTATTTCCC
  ACCACTGGCCGTTCAGGATCTGAAGACTATAGGTTTGAAGGTAGACTGGCGGCGCTCCTTCATCACCACG
  GACGTGAATCCTTACTACGATTCCTTTGTCAGGTGGCAGTTTTTAACTTTAAGAGAAAGAAATAAAATTA
  AATTTGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAAC
  TGGAGAGGGTGTTGGACCTCAGGAATATACTCTGGTCAAACTGAAGGTGCTTGAACCATATCCATCAAAA
  TTAAGCGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCTACACTCAGACCTGAGACTATGTTTGGGC
  AGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATCTTCAT
  CTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAACGGAGTGGTGCCGGTT
  GTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACGTGCTATAAGGTGG
  TCTACGTTCTCCCTATGCTGACCATTAAAGAGGATAAAGGCACTGGGGTGGTCACGAGCGTTCCTTCTGA
  TTCCCCGGATGATCTTGCAGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAACAAAGTTTGGAATT
  AGGGATGACATGGTCCTGCCGTTTGAACCGGTGCCAGTCCTGGAAATCCCAGGGATTGGAAATCTTCCTG
  CTGTGACTGTTTGTGATGAGCTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAAGCAAAGGA
  GAAATTGTATTTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGCTTTAAAGGACAGAAGATCCAA
  CATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAA
  AACAAGTGATGTCGAGGTCTGCAGATGAGTGCGTCGTGGCCCTGTGTGACCAGTGGTACTTGGATTATGG
  AGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGG
  AAGAATTTTGAAGCTTCCTTAGACTGGCTACAAGAACATGCTTGCTCAAGAACGTACGGTTTAGGCACTC
  GTCTGCCTTGGGATGAACAGTGGTTAATCGAATCTCTCTCGGATTCCACAATTTACATGGCGTTCTACAC
  AGTGGCACACCTGCTGCAGGGGGGTGACCTGAATGGGCAGGCGGAGTCTCCACTGGGCATCAGACCCCAG
  CAGATGACAAAGGACGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCTAAGACTCAGATCCCAA
  AGGAGAAGCTGGATCAGTTGAAGCAGGAGTTTGAGTTTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAA
  GGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAGTGAC
  AAGTGGCCCGTGTCTGTCCGAGCAAATGGACACCTGCTGCTCAACTCGGAGAAGATGTCAAAATCTACGG
  GCAACTTCCTCACTTTGAGCCAAGCTGTTGACAAATTTTCAGCTGACGGAATGCGCCTGGCTCTGGCTGA
  TGCTGGTGACACTGTTGAAGATGCTAACTTTGTGGAAGCCATGGCAGACGCAGGCATCCTCCGTCTGTAC
  ACATGGGTAGAGTGGGTCAAAGAAATGCTCGCCAGCTGCAGCAGCCTCAGAAGTGGACCTGCCGACTCAT
  TCAATGATCGTGTGTTTGCCAGTGAAATGAATGCAGGAATTATAAAAACAGATCAAAACTATGAAAAGAT
  GATGTTTAAAGAAGCTTTGAAAACAGGTTTTTTTGAGTTTCAGGCTGCGAAAGACAAATACCGTGAACTG
  GCCACGGAGGGCATGCACAGAGAGCTTGTGTTCCGCTTTATTGAAGTTCAGACAATCCTTCTGACTCCAT
  TCTGTCCACATTTGTGTGAGCACATCTGGACACTGCTTGGAAAGCCTGATTCAATCATGCATGCCTCATG
  GCCTGTGGCAGGTCCCGTGGATGAGTCCTTGATCCGTTCCTCACAGTATCTCATGGAAGTAGCACATGAC
  CTTAGATTACGGCTCAAGAATTACATGATGCCAGCTAAAGGGAAGAAGACTGACAAGCAGCCAGCTCAGA
  GGCCCTCACACTGCACCATCTATGTGGCAAAGAACTACCCCGTCTGGCAGCACATTACCCTGACAACCCT
  GCGGAGCCACTTTGAGGCCAACAATGGAAAGTTGCCTGATAACAAAGTCATTGCTAGTGAACTAGGCAGT
  TTGCCAGAGTTGAAGAAATATATGAAGAAAGTGATGCCATTCGTTGCTATGATTAAGGAAAACATGGAGA
  AGAAAGGGCCGAGAGTCTTGGATTTAGAGCTGGAGTTTGATGAGCAGGCAGTGCTTATGGAGAATATAGT
  CTACCTAACCAATTCTCTCGAGCTGGAACACATAGAAGTCAAGTTTGCCTCTGAGGCTGAAGATAAAGTC
  AGGGAGGAGTGCTGTCCTGGGAAGCCACTTAACGTTTTCAGAACAGAACCTGGTGTGCCAGTTTCCCTAG
  TAAATCCTCAGCCATCGAGTGGGCACTTCTCCACCAAAATCGACATCAGGCAAGGAGATAGCTGTGAGTC
  CATAATCAGACGCTTGATGAAAACGGACCGAGGGATCAAAGATCTTTCCAAAGTGAAGCTGATGCGGTTT
  GATGATCCTCTGTTGGGGCCTCGGCGAGTTCCGGTCCTGGGGAGAGAGCACAGTGAGAAGACACTCATTT
  CCGAGAACGCCGTTTTCCACGTGGACCTGGTGAGCAAGAAAGTCCACCTCACCGAGAACGGGCTGCGCAC
  GGACATCGGCGACACCATGGTCTACCTGGTTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211761 representing NM_134137
  Red=Cloning site Green=Tags(s)
  
  MAGRKGTAKVDFLKEIEKEAQQKWEAEKVFEVSASRLEKQKQSSKGKYFVTFPYPYMNGRLHLGHTFSLS
  KCEFAVGYQRLKGKSCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPEEEEEEEESSAKPGDIVVRD
  KAKGKKSKAAAKAGSSKYQWDIMKSLGLSDDDIVKFSEAEHWLDYFPPLAVQDLKTIGLKVDWRRSFITT
  DVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLEPYPSK
  LSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPV
  VKELMGEEILGASLSAPLTCYKVVYVLPMLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFGI
  RDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQ
  HVKKTIQKNMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEESR
  KNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLNGQAESPLGIRPQ
  QMTKDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSD
  KWPVSVRANGHLLLNSEKMSKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLY
  TWVEWVKEMLASCSSLRSGPADSFNDRVFASEMNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYREL
  ATEGMHRELVFRFIEVQTILLTPFCPHLCEHIWTLLGKPDSIMHASWPVAGPVDESLIRSSQYLMEVAHD
  LRLRLKNYMMPAKGKKTDKQPAQRPSHCTIYVAKNYPVWQHITLTTLRSHFEANNGKLPDNKVIASELGS
  LPELKKYMKKVMPFVAMIKENMEKKGPRVLDLELEFDEQAVLMENIVYLTNSLELEHIEVKFASEAEDKV
  REECCPGKPLNVFRTEPGVPVSLVNPQPSSGHFSTKIDIRQGDSCESIIRRLMKTDRGIKDLSKVKLMRF
  DDPLLGPRRVPVLGREHSEKTLISENAVFHVDLVSKKVHLTENGLRTDIGDTMVYLVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134137
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134137.3
• RefSeq Size: 3845 bp
• RefSeq ORF: 3537 bp
• Locus ID: 107045
• UniProt ID: Q8BMJ2
• Cytogenetics: 18 B3
• MW: 134.6 kDa
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.[UniProtKB/Swiss-Prot Function]