Actn4 (NM_021895) Mouse Tagged ORF Clone

CAT#: MR211156

Actn4 (Myc-DDK-tagged) - Mouse actinin alpha 4 (Actn4)

Product Images

Circular map for MR211156

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actn4
• Synonyms: C77391
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211156 representing NM_021895
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACTACCACGCAGCGAACCAGGCGTACCAGTACGGCCCGAACAGCGGCGGCGGCAATGGCGCGG
  GTGGCGGCGGAAGCATGGGCGACTACATGGCCCAGGAGGATGACTGGGACCGGGACCTGCTGTTGGACCC
  GGCCTGGGAGAAGCAGCAGCGCAAGACCTTCACAGCCTGGTGCAACTCTCATCTTCGGAAGGCTGGCACT
  CAGATCGAGAATATCGATGAGGACTTCAGGGATGGGCTCAAACTTATGCTTCTGTTGGAGGTCATTTCAG
  GGGAGCGTTTGCCTAAGCCAGAGCGGGGGAAGATGCGAGTGCACAAGATCAACAATGTAAACAAAGCCCT
  GGACTTCATCGCCAGCAAGGGAGTCAAGCTGGTGTCCATCGGGGCAGAAGAGATTGTGGATGGCAATGCA
  AAGATGACCCTGGGAATGATCTGGACCATCATCCTCAGATTTGCCATCCAGGACATCTCTGTGGAAGAGA
  CCTCTGCTAAGGAAGGACTCCTCCTCTGGTGCCAGAGAAAGACAGCCCCATATAAAAACGTCAACGTCCA
  GAACTTCCATATCAGCTGGAAGGACGGGCTGGCCTTCAATGCACTCATCCACCGGCACAGGCCTGAGCTG
  ATTGAGTATGACAAGCTGCGGAAGGATGATCCAGTCACCAACCTAAACAATGCATTTGAAGTGGCTGAGA
  AATACCTCGATATCCCCAAGATGTTGGATGCTGAGGACATCGTGAACACAGCCCGGCCCGACGAGAAGGC
  CATAATGACATATGTGTCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAGGCTGAGACCGCTGCCAAC
  CGGATCTGCAAGGTGCTGGCTGTGAACCAGGAAAATGAGCACCTGATGGAAGACTATGAACGCCTGGCCA
  GTGATCTTCTAGAGTGGATTCGGCGCACCATCCCCTGGCTGGAGGACCGGGTGCCTCAGAAGACCATCCA
  GGAAATGCAGCAGAAGCTGGAGGACTTCCGAGACTATAGGCGTGTGCACAAGCCGCCCAAGGTGCAGGAG
  AAGTGTCAGCTGGAGATCAACTTCAACACACTGCAGACCAAGCTGCGGCTCAGCAACCGGCCTGCCTTCA
  TGCCCTCCGAGGGCAGGATGGTCTCCGACATCAACAATGGATGGCAGCACCTGGAGCAGGCTGAGAAGGG
  CTATGAAGAATGGCTGCTGAATGAAATCCGTAGGCTGGAACGGCTTGACCACCTGGCAGAGAAGTTCCGG
  CAGAAGGCTTCCATCCACGAGGCCTGGACTGATGGGAAGGAGGCCATGCTGAAGCAACGGGACTACGAGA
  CAGCCACCCTGTCAGACATCAAAGCTCTGATCCGAAAACATGAGGCCTTTGAAAGTGACCTGGCTGCACA
  CCAGGACCGAGTGGAGCAGATTGCTGCAATTGCTCAGGAGCTCAACGAGCTGGACTACTATGACTCCCAC
  AACGTCAACACACGGTGCCAGAAGATCTGCGACCAGTGGGATAACCTGGGCTCTCTGACACACAGTCGCA
  GGGAAGCCTTGGAGAAAACAGAGAAACAGCTAGAGACCATCGACCAGCTACATTTGGAGTATGCCAAGCG
  GGCTGCACCCTTCAACAACTGGATGGAGAGTGCCATGGAGGACCTGCAGGACATGTTCATCGTCCACACC
  ATCGAGGAGATCGAGGGCCTGATCTCAGCCCATGACCAGTTCAAGTCCACCCTGCCAGATGCTGACAGGG
  AGCGTGAGGCCATCCTGGCCATCCACAAGGAGGCCCAGAGGATCGCTGAGAGCAATCACATCAAGCTGTC
  GGGCAGCAATCCCTACACCACTGTTACCCCCCAGATCATCAACTCCAAGTGGGAGAAGGTGCAGCAGCTG
  GTGCCAAAGAGGGACCATGCACTCCTGGAGGAGCAGAGCAAGCAGCAATCCAATGAGCACCTTCGCCGAC
  AGTTCGCCAGCCAAGCCAATATGGTGGGGCCGTGGATCCAGACCAAGATGGAGGAGATCGGGCGCATCTC
  CATTGAGATGAACGGGACTCTGGAAGACCAGCTGAGCCACCTGAAGCAGTACGAGCGCAGCATCGTGGAC
  TACAAGCCCAGCCTGGACCTGCTGGAGCAGCAGCACCAGCTCATCCAGGAGGCCCTCATCTTCGACAACA
  AGCACACCAACTACACAATGGAGCATATCCGTGTGGGCTGGGAGCAGCTGCTCACGACCATTGCCCGCAC
  CATCAATGAGGTGGAAAACCAGATCCTCACCCGAGATGCCAAGGGTATCAGCCAGGAGCAGATGCAGGAG
  TTCCGGGCATCCTTCAACCATTTTGACAAGGACCATGGCGGGGCACTGGGGCCTGAGGAATTCAAGGCCT
  GCCTCATCAGCCTGGGCTATGACGTGGAGAATGACCGGCAGGGTGATGCTGAGTTCAACCGGATCATGAG
  TGTGGTTGATCCCAACCATAGTGGCCTCGTGACCTTCCAAGCCTTCATTGACTTCATGTCAAGGGAGACC
  ACAGACACAGACACAGCTGATCAGGTCATCGCCTCCTTCAAGGTCCTGGCAGGAGACAAGAACTTCATCA
  CTGCTGAGGAACTGCGGAGAGAGCTGCCCCCCGACCAGGCCGAGTACTGCATCGCCCGCATGGCACCCTA
  CCAGGGGCCTGATGCTGCTCCTGGCGCCCTTGACTACAAGTCCTTCTCCACAGCCCTCTATGGGGAGAGC
  GACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211156 representing NM_021895
  Red=Cloning site Green=Tags(s)
  
  MVDYHAANQAYQYGPNSGGGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGT
  QIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNA
  KMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPEL
  IEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAAN
  RICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQE
  KCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFR
  QKASIHEAWTDGKEAMLKQRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSH
  NVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHT
  IEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQL
  VPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVD
  YKPSLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQE
  FRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRET
  TDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGES
  DL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021895
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021895.3
• RefSeq Size: 3859 bp
• RefSeq ORF: 2739 bp
• Locus ID: 60595
• UniProt ID: P57780
• Cytogenetics: 7 B1
• MW: 105.4 kDa
• Gene Summary: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity).[UniProtKB/Swiss-Prot Function]