Actn1 (NM_134156) Mouse Tagged ORF Clone

CAT#: MR211081

Actn1 (Myc-DDK-tagged) - Mouse actinin, alpha 1 (Actn1)

Product Images

Circular map for MR211081

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actn1
• Synonyms: 3110023F10Rik; Actn1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211081 representing NM_134156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTATGATTCCCAGCAGACCAACGACTACATGCAGCCTGAAGAGGACTGGGATCGGGACCTGC
  TCCTGGATCCGGCCTGGGAGAAGCAGCAGAGGAAGACGTTCACAGCCTGGTGCAACTCCCACCTGCGCAA
  AGCGGGGACACAGATCGAGAACATCGAAGAGGACTTCCGAGATGGCCTGAAGCTCATGCTGCTCCTGGAG
  GTCATCTCAGGTGAACGCTTGGCCAAGCCAGAGAGAGGCAAGATGAGAGTGCACAAGATCTCCAACGTCA
  ACAAGGCCTTGGATTTCATAGCCAGCAAAGGGGTCAAGCTGGTGTCCATCGGAGCTGAAGAAATTGTGGA
  TGGGAATGTGAAGATGACCCTGGGCATGATCTGGACCATCATTCTCCGCTTTGCCATCCAGGACATCTCC
  GTGGAAGAGACTTCAGCTAAAGAAGGGTTACTCCTGTGGTGTCAGAGGAAGACAGCCCCATATAAAAATG
  TCAACATCCAAAACTTCCACATCAGTTGGAAAGATGGTCTTGGTTTCTGTGCCTTGATCCACCGACACCG
  GCCCGAGTTGATTGACTATGGAAAGCTTCGAAAGGATGATCCACTCACAAACCTGAACACGGCCTTTGAT
  GTGGCAGAGAGGTTCCTGGACATCCCTAAGATGCTGGATGCTGAAGACATCGTCGGAACTGCCCGGCCAG
  ATGAGAAAGCCATTATGACTTATGTGTCTAGCTTCTATCATGCCTTCTCTGGAGCCCAGAAGGCAGAAAC
  AGCAGCCAATCGCATCTGCAAAGTGTTGGCGGTCAATCAAGAGAACGAGCAGCTTATGGAAGACTATGAG
  AAGCTGGCCAGTGATCTGTTGGAGTGGATCCGCCGCACCATCCCATGGCTGGAGAATCGGGTGCCTGAGA
  ACACCATGCATGCCATGCAGCAGAAGCTGGAGGACTTCCGAGACTATAGACGCCTGCACAAGCCGCCCAA
  GGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTTAACACGCTGCAGACCAAGCTGCGGCTCAGCAACCGG
  CCTGCCTTCATGCCCTCCGAGGGCAGGATGGTCTCGGATATCAACAATGCCTGGGGCTGCCTGGAACAGG
  CCGAGAAGGGCTACGAGGAGTGGCTGCTGAATGAGATCCGGAGGCTAGAGAGGTTGGACCACCTGGCAGA
  GAAGTTCCGGCAGAAGGCCTCCATCCATGAAGCCTGGACGGATGGCAAAGAAGCCATGCTTCGGCAGAAG
  GATTATGAGACAGCCACCCTGTCCGAGATCAAGGCCTTGCTCAAGAAGCACGAGGCTTTTGAGAGTGATC
  TGGCTGCCCACCAGGACCGCGTGGAGCAGATTGCGGCCATTGCCCAGGAGCTTAATGAGCTGGACTACTA
  TGACTCGCCAAGTGTCAACGCTCGTTGCCAAAAGATCTGTGACCAGTGGGACAATCTAGGGGCTCTCACT
  CAGAAGCGGAGGGAAGCTTTAGAGCGAACAGAGAAGCTGCTGGAAACCATCGACCAGCTCTACTTAGAGT
  ACGCCAAGAGGGCTGCACCCTTCAACAATTGGATGGAGGGAGCCATGGAGGACCTGCAGGACACCTTCAT
  CGTACATACCATCGAGGAGATCCAGGGACTGACCACAGCCCATGAGCAGTTCAAGGCCACCCTCCCGGAT
  GCAGACAAGGAGCGCCTCGCCATTCTGGGCATCCACAATGAAGTGTCCAAGATCGTCCAGACCTATCATG
  TCAACATGGCAGGCACCAACCCCTATACAACCATCACGCCTCAGGAGATCAATGGCAAATGGGACCATGT
  ACGGCAGCTAGTGCCCCGGAGGGACCAGGCTCTCACAGAGGAACATGCAAGGCAGCAGCACAATGAGAGG
  CTACGCAAACAGTTTGGCGCACAAGCCAATGTCATTGGGCCCTGGATCCAGACCAAGATGGAGGAGATTG
  GGAGGATTTCCATTGAGATGCACGGCACCCTGGAGGACCAACTCAGCCACCTGCGGCAGTATGAGAAGAG
  CATCGTCAATTACAAGCCAAAGATTGACCAGCTGGAGTGCGACCACCAGCTCATCCAGGAGGCACTTATC
  TTTGACAATAAGCACACCAACTACAACATGGAGCATATCCGTGTGGGCTGGGAGCAGCTGCTCACCACCA
  TTGCCAGGACCATCAATGAAGTGGAGAACCAGATCTTGACCCGGGATGCCAAAGGCATCAGCCAGGAACA
  GATGAACGAATTCCGCGCCTCTTTCAACCACTTTGACCGGGATCACTCCGGCACGTTGGGTCCCGAAGAG
  TTCAAAGCCTGCCTCATCAGCTTGGGTTATGATATTGGCAACGACCCCCAGGGAGAGGCAGAGTTTGCCC
  GAATCATGAGCATTGTAGACCCCAACCGCTTGGGGGTAGTGACGTTCCAGGCCTTCATCGACTTTATGTC
  CCGAGAGACGGCAGATACTGATACTGCAGATCAGGTCATGGCTTCCTTCAAGATCCTGGCTGGAGATAAG
  AATTACATCACAGAGGATGAGCTGCGCCGTGAGCTGCCCCCTGACCAAGCCGAGTACTGCATCGCAAGAA
  TGGCCCCCTACGCGGGCCCCGACTCCGTGCCCGGTGCTTTAGACTACATGTCCTTCTCCACGGCACTGTA
  TGGCGAGAGCGACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211081 representing NM_134156
  Red=Cloning site Green=Tags(s)
  
  MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLE
  VISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDIS
  VEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFD
  VAERFLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYE
  KLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNR
  PAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQK
  DYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALT
  QKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPD
  ADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNER
  LRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLECDHQLIQEALI
  FDNKHTNYNMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEE
  FKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDK
  NYITEDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALYGESDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134156
• ORF Size: 2676 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134156.3
• RefSeq Size: 3705 bp
• RefSeq ORF: 2679 bp
• Locus ID: 109711
• UniProt ID: Q7TPR4
• Cytogenetics: 12 36.49 cM
• MW: 103.5 kDa
• Gene Summary: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).[UniProtKB/Swiss-Prot Function]