Pml (NM_008884) Mouse Tagged ORF Clone

CAT#: MR210910

Pml (Myc-DDK-tagged) - Mouse promyelocytic leukemia (Pml), transcript variant 1

Product Images

Circular map for MR210910

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pml
• Synonyms: 1200009E24Rik; AI661194; Trim19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210910 representing NM_008884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTGAACCAGTTTCCGTGCAGAAGGTACCTGCACCCCCTGGATCTCCCTGTCGACAACAGGACT
  CTGCCCTGACCCCCACGCCCACCATGCCTCCCCCAGAGGAACCCTCCGAAGACTATGAACACAGCCAAAG
  CCCTGCAGAGCAAGCCATACAGGAGGAATTTCAGTTTCTGCGCTGCCCGAGCTGCCAGGCCCAAGCCAAG
  TGCCCCAAACTGCTGCCTTGCCTGCACACGCTGTGCTCCGGATGCCTGGAGGCGCCTGGCCTGCAGTGCC
  CCATCTGCAAGGCTCCTGGGCAGGCCGATGCTAATGGGGAGGCCCTGGATAACGTGTTCTTCGAGAGCCT
  GCAGCGGCGCCTGGCGGTGTTCCGGCAGATCGTGGATGCTCAGGCTGCGTGCACCCGCTGCAAAGGCTTG
  GCCGACTTCTGGTGTTTCGAGTGTGAACAGCTCATTTGCAGCAAGTGCTTCGAAGCACACCAGTGGTACC
  TCAAGCATGAGGCCCGGCCCCTGGCTGATCTCCGCGACAATTCAGTGAGCAGCTTCCTCGACAGTACGCG
  CAAGTCCAATATCTTCTGCTCCAATACCAACCACCGCAACCCTGCGCTGACTGACATCTACTGCCGAGGC
  TGCGCCAAGCCTCTGTGTTGCACATGCGCGCTCCTGGACCGCAACCACAGCCATCTCCATTGCGATATTG
  GTGAGGAGATTCAGCAGTGGCATGAGGAACTAGGCACCATGACACAGACTCTGGAGGAGCAGGGCAGAAC
  CTTCGACAGTGCCCATGCACAGATGTGCTCAGCTATAGGACAGCTGGACCACGCACGCGCAGACATTGAG
  AAGCAGATCCGCGCACGCGTGCGCCAGGTGGTAGACTACGTGCAGGCTCAGGAGCGCGAGCTGCTCGAGG
  CGGTGAATGACCGCTACCAGCGCGACTACCAGGAAATAGCTGGCCAGCTGAGCTGCCTGGAAGCTGTGCT
  GCAGCGCATCCGCACTAGTGGGGCGCTGGTCAAGAGGATGAAGCTCTATGCCTCCGACCAGGAGGTGCTG
  GATATGCACAGCTTTCTGCGCAAGGCACTCTGTAGCTTGCGCCAGGAGGAGCCCCAGAACCAGAAAGTCC
  AGCTGCTCACCAGAGGTTTCGAGGAGTTCAAGCTGTGCCTGCAGGACTTCATCTCCTGCATCACCCAGAG
  GATAAATGCAGCTGTAGCCAGCCCAGAGGCAGCCAGCAATCAACCAGAGGCAGCCAGCACTCACCCAGTG
  ACAACCAGCACGCCTGAGGACCTTGAGCAGGAGGCTTCTCAGACAGTCGGCTCCATGAAGAGGAAGTGCT
  CCCACGAAGATTGCTCCAGGAAGATCATCAAGATGGAGTCCACAGAGGAGAACGAGGACAGGTTGGCCAC
  AAGCTCCCCCGAGCAGTCCTGGCCCAGCACTTTCAAGGCCACCTCCCCTCCCCATCTGGATGGGACTTCC
  AACCCTGAGAGCACCGTCCCTGAAAAAAAGATCCTCCTGCCCAACAACAATCATGTTACCAGTGACACAG
  GGGAAACAGAGGAGCGAGTTGTGGTGATCAGCAGCTCAGAGGATTCGGACACCGAGAATCTGTCCTCCCA
  CGAGTTGGACGATAGCAGCAGTGAGTCCAGCAGCCTGCAGCTGGAGGGCCCCAATTCCCTCAAGGCCTTG
  GATGAGAGCCTCGCCGAACCCCACTTAGAAGACAGGACCCTGGTTTTCTTTGACCTCAAGATTGACAATG
  AAACCCAGAAAATTAGCCAGCTGGCCGCGGTGAACCGGGAAAGCAAGTTCCGTGTGCTCATCCAGCCCGA
  GGCCTTCAGTGTCTACTCCAAAGCTGTCTCCCTGGAGGCGGGGCTCCGGCACTTCCTCAGCTTCCTCACC
  ACCATGCACCGTCCCATCTTGGCGTGTTCTAGGCTGTGGGGGCCGGGACTCCCCATCTTCTTCCAGACCC
  TGAGTGATATTAACAAGCTGTGGGAATTCCAGGACACCATCTCAGGTTTCTTGGCCGTGCTGCCCCTCAT
  CCGGGAACGCATACCCGGCGCTAGCAGCTTCAAACTTGGGAACCTAGCCAAGACCTACCTGGCGAGAAAC
  ATGAGCGAACGCAGCGCTCTGGCTTCTGTGCTGGCCATGAGGGACTTGTGCTGCCTCCTTGAGATCTCCC
  CAGGACTGCCGCTGGCCCAGCATATCTACTCCTTTAGTAGCTTGCAGTGTTTTGCTTCCCTGCAGCCCCT
  AATTCAGGCCAGCGTCCTGCCACAGTCCGAGGCCCGCCTCTTGGCCCTCCACAATGTGAGCTTTGTAGAG
  TTACTGAATGCATATCGCACCAACAGGCAAGAAGGCTTGAAGAAGTATGTCCACTATCTGAGCCTGCAGA
  CCACCCCGTTGTCATCGTCGGCTTCCACCCAAGTTGCCCAATTCCTGCAGGCTCTTAGCACCCACATGGA
  AGGACTGTTGGAAGGCCATGCCCCTGCTGGGGCAGAAGGCAAAGCTGAAAGTAAGGGATGCCTGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210910 representing NM_008884
  Red=Cloning site Green=Tags(s)
  
  METEPVSVQKVPAPPGSPCRQQDSALTPTPTMPPPEEPSEDYEHSQSPAEQAIQEEFQFLRCPSCQAQAK
  CPKLLPCLHTLCSGCLEAPGLQCPICKAPGQADANGEALDNVFFESLQRRLAVFRQIVDAQAACTRCKGL
  ADFWCFECEQLICSKCFEAHQWYLKHEARPLADLRDNSVSSFLDSTRKSNIFCSNTNHRNPALTDIYCRG
  CAKPLCCTCALLDRNHSHLHCDIGEEIQQWHEELGTMTQTLEEQGRTFDSAHAQMCSAIGQLDHARADIE
  KQIRARVRQVVDYVQAQERELLEAVNDRYQRDYQEIAGQLSCLEAVLQRIRTSGALVKRMKLYASDQEVL
  DMHSFLRKALCSLRQEEPQNQKVQLLTRGFEEFKLCLQDFISCITQRINAAVASPEAASNQPEAASTHPV
  TTSTPEDLEQEASQTVGSMKRKCSHEDCSRKIIKMESTEENEDRLATSSPEQSWPSTFKATSPPHLDGTS
  NPESTVPEKKILLPNNNHVTSDTGETEERVVVISSSEDSDTENLSSHELDDSSSESSSLQLEGPNSLKAL
  DESLAEPHLEDRTLVFFDLKIDNETQKISQLAAVNRESKFRVLIQPEAFSVYSKAVSLEAGLRHFLSFLT
  TMHRPILACSRLWGPGLPIFFQTLSDINKLWEFQDTISGFLAVLPLIRERIPGASSFKLGNLAKTYLARN
  MSERSALASVLAMRDLCCLLEISPGLPLAQHIYSFSSLQCFASLQPLIQASVLPQSEARLLALHNVSFVE
  LLNAYRTNRQEGLKKYVHYLSLQTTPLSSSASTQVAQFLQALSTHMEGLLEGHAPAGAEGKAESKGCLA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008884
• ORF Size: 2517 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008884.5, NP_032910.3
• RefSeq Size: 5240 bp
• RefSeq ORF: 2520 bp
• Locus ID: 18854
• UniProt ID: Q60953
• Cytogenetics: 9 31.63 cM
• MW: 93.7 kDa
• Gene Summary: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum. Regulates RB1 phosphorylation and activity. Acts as both a negative regulator of PPARGC1A acetylation and a potent activator of PPAR signaling and fatty acid oxidation. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates PER2 nuclear localization and circadian function. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. Required for normal development of the brain cortex during embryogenesis. Plays a role in granulopoiesis or monopoiesis of myeloid progenitor cells. May play a role regulating stem and progenitor cell fate in tissues as diverse as blood, brain and breast. Shows antiviral activity towards lymphocytic choriomeningitis virus (LCMV) and the vesicular stomatitis virus (VSV).[UniProtKB/Swiss-Prot Function]