Abcb10 (NM_019552) Mouse Tagged ORF Clone

CAT#: MR210225

Abcb10 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR210225

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb10
• Synonyms: ABC-m; Abc-me; Abcb12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210225 representing NM_019552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCCCTTCTGCTAGGGCGCTACTGCTGATTCCGCGTCGGGGCCCTGCCGTGCGAGCGTGGGCCC
  CGGCCGTCTCCTCTCGGATATGGCTGGCTTCTGAATGGACCCCGCTCGTACGCGCGTGGACCTCTCTGAT
  CCACAAGCCGGGTTCGGGCCTCCGCTTTCCCGCGCCCCTATCCGGGCTGCCTGGCGGCGTGGGGCAGTGG
  GCCACCTCCTCGGGGGCCCGCAGGTGCTGGGTGCTGGCAGGACCCCGCGCCGCACATCCCCTGTTCGCCA
  GGCTCCAGGGTGCAGCTGCCACCGGTGTGCGAGACCTTGGGAACGACTCGCAGCGGCGTCCCGCGGCGAC
  CGGGCGCTCAGAAGTATGGAAGCTCCTAGGGCTGGTGCGCCCCGAGCGCGGGAGACTGTCAGCTGCAGTT
  GGGTTTCTGGCTGTGTCCAGTGTCATCACCATGTCTGCCCCCTTCTTCCTGGGAAGGATCATTGATGTCA
  TCTATACCAACCCAAGCGAGGGCTATGGCGATAGCTTGACTCGGCTCTGTGCTGTGCTCACCTGTGTGTT
  CCTGTGCGGCGCTGCTGCCAACGGCATTCGTGTCTACCTCATGCAGTCTTCAGGTCAGAGCATTGTGAAC
  AGGCTGCGCACCTCTCTGTTCTCCTCCATTCTAAGGCAAGAGGTTGCTTTCTTTGACAAGACCCGCACAG
  GCGAATTAATTAACCGCCTCTCCTCGGATACCGCACTCCTGGGGCGCTCCGTGACTGAGAACCTCTCCGA
  TGGCCTGAGAGCTGGGGCCCAGGCCTCCGTTGGTGTTGGCATGATGTTTTTTGTGTCACCAAGTCTGGCC
  ACCTTTGTTCTGAGTGTGGTACCGCCCATCTCTGTCCTCGCTGTGATTTATGGGCGATATCTACGGAAAC
  TGTCCAAAGCCACACAGGACTCCTTGGCAGAAGCCACACAGCTAGCCGAGGAGCGGATTGGAAACATAAG
  AACCATCCGAGCTTTTGGGAAGGAGATGACGGAAGTGGAGAAGTACACCGGCCGAGTGGACCAGTTGCTG
  CAGCTGGCACAGAAGGAGGCCTTGGCACGAGCCGGCTTCTTTGGAGCAGCTGGGCTCTCAGGAAACCTGA
  TTGTGCTGTCTGTCCTGTACAAAGGAGGCCTGCTGATGGGCAGTGCCCATATGACAGTGGGCGAGCTCTC
  TTCCTTCCTCATGTACGCTTTCTGGGTTGGATTGAGCATCGGAGGACTGAGTTCATTCTACTCCGAGCTG
  ATGAAAGGCCTGGGTGCTGGTGGGCGGCTCTGGGAGCTGCTGGAGAGACAGCCACGGCTGCCTTTTAACG
  AGGGGATGGTGTTAGATGAGAAAACCTTCCAGGGTGCCCTGGAGTTCAGAAACGTGCACTTCACATACCC
  TGCTCGCCCAGAGGTGTCCGTATTCCAGGATTTCAGTCTTTCCATCCCATCTGGATCTGTCACAGCGCTG
  GTTGGCCCAAGTGGTTCTGGGAAATCAACAGTGGTTTCGCTCCTGCTGCGGTTGTACGACCCTAATTCTG
  GAACAGTCAGCCTTGATGGCCATGACATTCGTCAGCTAAACCCCGTCTGGCTGAGATCCAAGATTGGGAC
  AGTGAGTCAGGAGCCAGTCCTGTTCTCCTGCTCTGTCGCTGAGAACATTGCCTATGGAGCTGACAACCTC
  TCCTCGGTCACAGCCCAGCAGGTGGAGAGGGCCGCAGAGGTGGCCAACGCCGCTGAGTTCATCCGAAGCT
  TCCCACAGGGCTTCGACACTGTGGTCGGGGAGAAGGGCATCCTCCTGTCAGGTGGGCAGAAGCAGAGGAT
  TGCAATAGCCAGAGCCCTGCTGAAGAACCCCAAAATTCTTCTCCTTGATGAAGCAACCAGTGCACTGGAT
  GCCGAAAATGAGCACCTGGTCCAGGAGGCCCTTGACCGGCTGATGGAAGGGAGAACCGTGCTGATCATAG
  CCCACCGCCTGTCCACCATTAAGAATGCCAACTTTGTTGCTGTCCTTGACCACGGAAAGATCTGTGAACA
  CGGGACACATGAAGAGCTGCTCCTGAAGCCCAATGGGCTTTACAGAAAACTGATGAACAAGCAGAGTTTC
  CTGTCATATAATGGAGCAGAGCAGTTCCTGGAACCTGCACGGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210225 representing NM_019552
  Red=Cloning site Green=Tags(s)
  
  MRAPSARALLLIPRRGPAVRAWAPAVSSRIWLASEWTPLVRAWTSLIHKPGSGLRFPAPLSGLPGGVGQW
  ATSSGARRCWVLAGPRAAHPLFARLQGAAATGVRDLGNDSQRRPAATGRSEVWKLLGLVRPERGRLSAAV
  GFLAVSSVITMSAPFFLGRIIDVIYTNPSEGYGDSLTRLCAVLTCVFLCGAAANGIRVYLMQSSGQSIVN
  RLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTALLGRSVTENLSDGLRAGAQASVGVGMMFFVSPSLA
  TFVLSVVPPISVLAVIYGRYLRKLSKATQDSLAEATQLAEERIGNIRTIRAFGKEMTEVEKYTGRVDQLL
  QLAQKEALARAGFFGAAGLSGNLIVLSVLYKGGLLMGSAHMTVGELSSFLMYAFWVGLSIGGLSSFYSEL
  MKGLGAGGRLWELLERQPRLPFNEGMVLDEKTFQGALEFRNVHFTYPARPEVSVFQDFSLSIPSGSVTAL
  VGPSGSGKSTVVSLLLRLYDPNSGTVSLDGHDIRQLNPVWLRSKIGTVSQEPVLFSCSVAENIAYGADNL
  SSVTAQQVERAAEVANAAEFIRSFPQGFDTVVGEKGILLSGGQKQRIAIARALLKNPKILLLDEATSALD
  AENEHLVQEALDRLMEGRTVLIIAHRLSTIKNANFVAVLDHGKICEHGTHEELLLKPNGLYRKLMNKQSF
  LSYNGAEQFLEPARA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019552
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019552.2, NP_062425.1
• RefSeq Size: 4325 bp
• RefSeq ORF: 2148 bp
• Locus ID: 56199
• UniProt ID: Q9JI39
• Cytogenetics: 8 E2
• MW: 77.6 kDa
• Gene Summary: This gene encodes a member of the ATP-binding cassette superfamily of transporters. ATP-binding cassette proteins transport various molecules across extra- and intra-cellular membranes. The encoded protein is localized to the mitochondrial inner membrane where it interacts with and stabilizes mitoferrin-1, and is important for heme biosynthesis. Additional evidence suggests the encoded protein is involved in oxidative stress protection and erythropoisesis. [provided by RefSeq, May 2013]