Masp2 (NM_001003893) Mouse Tagged ORF Clone

CAT#: MR210030

Masp2 (Myc-DDK-tagged) - Mouse mannan-binding lectin serine peptidase 2 (Masp2), transcript variant 1

Product Images

Circular map for MR210030

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Masp2
• Synonyms: MAp19; MASP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTACTCATCTTCCTGGGTCTGCTGTGGAGTTTGGTGGCCACACTTCTGGGTTCAAAGTGGCCTG
  AACCTGTATTCGGGCGCCTGGTGTCCCCTGGCTTCCCAGAGAAGTATGCTGACCATCAAGATCGATCCTG
  GACACTGACTGCACCCCCTGGCTACCGCCTGCGCCTCTACTTCACCCACTTTGACCTGGAACTCTCTTAC
  CGCTGCGAGTATGACTTTGTCAAGTTGAGCTCAGGGACCAAGGTGCTGGCCACACTGTGTGGGCAGGAGA
  GTACAGACACTGAGCAGGCACCTGGCAATGACACCTTCTACTCACTGGGTCCCAGCCTAAAGGTCACCTT
  CCACTCCGACTACTCCAATGAGAAGCCGTTCACAGGGTTTGAGGCCTTCTATGCAGCGGAGGATGTGGAT
  GAATGCAGAGTGTCTCTGGGAGACTCAGTCCCTTGTGACCATTATTGCCACAACTACTTGGGCGGCTACT
  ATTGCTCCTGCAGAGCGGGCTACGTTCTCCACCAGAACAAGCACACGTGCTCAGCCCTTTGTTCAGGCCA
  GGTGTTCACAGGAAGATCTGGGTATCTCAGTAGCCCTGAGTACCCACAGCCATACCCCAAGCTCTCCAGC
  TGCACCTACAGCATCCGCCTGGAGGACGGCTTCAGTGTCATCCTGGACTTCGTGGAGTCCTTCGATGTGG
  AGACGCACCCTGAAGCCCAGTGCCCCTATGACTCCCTCAAGATTCAAACAGACAAGGGGGAACACGGCCC
  ATTTTGTGGGAAGACGCTGCCTCCCAGGATTGAAACTGACAGCCACAAGGTGACCATCACCTTTGCCACT
  GACGAGTCGGGGAACCACACAGGCTGGAAGATACACTACACAAGCACAGCACGGCCCTGCCCTGATCCAA
  CGGCGCCACCTAATGGCAGCATTTCACCTGTGCAAGCCATATATGTCCTGAAGGACAGGTTTTCTGTCTT
  CTGCAAGACAGGCTTCGAGCTTCTGCAAGGTTCTGTCCCCCTGAAATCATTCACTGCTGTCTGTCAGAAA
  GATGGATCTTGGGACCGGCCGATGCCAGAGTGCAGCATTATTGATTGTGGCCCTCCTGATGACCTACCCA
  ATGGCCATGTGGACTATATCACAGGCCCTGAAGTGACTACCTACAAAGCTGTGATTCAGTACAGCTGTGA
  AGAGACTTTCTACACAATGAGCAGCAATGGTAAATATGTGTGTGAGGCTGATGGATTCTGGACGAGCTCC
  AAAGGAGAAAAACTCCCCCCGGTTTGTGAGCCTGTTTGTGGGCTGTCCACACACACTATAGGAGGACGCA
  TAGTTGGAGGGCAGCCTGCAAAGCCTGGTGACTTTCCTTGGCAAGTCTTGTTGCTGGGTCAAACTACAGC
  AGCAGCAGGTGCACTTATACATGACAATTGGGTCCTAACAGCCGCTCATGCTGTATATGAGAAAAGAATG
  GCAGCGTCCTCCCTGAACATCCGAATGGGCATCCTCAAAAGGCTCTCACCTCATTACACTCAAGCCTGGC
  CCGAGGAAATCTTTATACATGAAGGCTACACTCACGGTGCTGGTTTTGACAATGATATAGCATTGATTAA
  ACTCAAGAACAAAGTCACAATCAACGGAAGCATCATGCCTGTTTGCCTACCGCGAAAAGAAGCTGCATCC
  TTAATGAGAACAGACTTCACTGGAACTGTGGCTGGCTGGGGGTTAACCCAGAAGGGGCTTCTTGCTAGAA
  ACCTAATGTTTGTGGACATACCAATTGCTGACCACCAAAAATGTACCGCCGTGTATGAAAAGCTCTATCC
  AGGAGTAAGAGTAAGCGCTAACATGCTCTGTGCTGGCTTAGAGACTGGTGGCAAGGACAGCTGCAGAGGT
  GACAGTGGGGGGGCATTAGTGTTTCTAGATAATGAGACACAGCGATGGTTTGTGGGAGGAATAGTTTCCT
  GGGGTTCCATTAATTGTGGGGCGGCAGACCAGTATGGGGTCTACACAAAAGTCATCAACTATATTCCCTG
  GATTGAGAACATAATAAGTAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLLIFLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYADHQDRSWTLTAPPGYRLRLYFTHFDLELSY
  RCEYDFVKLSSGTKVLATLCGQESTDTEQAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVD
  ECRVSLGDSVPCDHYCHNYLGGYYCSCRAGYVLHQNKHTCSALCSGQVFTGRSGYLSSPEYPQPYPKLSS
  CTYSIRLEDGFSVILDFVESFDVETHPEAQCPYDSLKIQTDKGEHGPFCGKTLPPRIETDSHKVTITFAT
  DESGNHTGWKIHYTSTARPCPDPTAPPNGSISPVQAIYVLKDRFSVFCKTGFELLQGSVPLKSFTAVCQK
  DGSWDRPMPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVIQYSCEETFYTMSSNGKYVCEADGFWTSS
  KGEKLPPVCEPVCGLSTHTIGGRIVGGQPAKPGDFPWQVLLLGQTTAAAGALIHDNWVLTAAHAVYEKRM
  AASSLNIRMGILKRLSPHYTQAWPEEIFIHEGYTHGAGFDNDIALIKLKNKVTINGSIMPVCLPRKEAAS
  LMRTDFTGTVAGWGLTQKGLLARNLMFVDIPIADHQKCTAVYEKLYPGVRVSANMLCAGLETGGKDSCRG
  DSGGALVFLDNETQRWFVGGIVSWGSINCGAADQYGVYTKVINYIPWIENIISNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003893
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003893.2, NP_001003893.1
• RefSeq Size: 3061 bp
• RefSeq ORF: 2058 bp
• Locus ID: 17175
• UniProt ID: Q91WP0
• Cytogenetics: 4 E2
• MW: 75.5 kDa
• Gene Summary: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase (By similarity).[UniProtKB/Swiss-Prot Function]