Raf1 (NM_029780) Mouse Tagged ORF Clone

CAT#: MR209737

Raf1 (Myc-DDK-tagged) - Mouse v-raf-leukemia viral oncogene 1 (Raf1)

Product Images

Circular map for MR209737

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Raf1
• Synonyms: 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; Raf-1; v-Raf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209737 representing NM_029780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGCTTTGGACTCAAAGATGCGGTGTTTGATG
  GCTCCAGCTGCATCTCCCCTACCATTGTTCAGCAGTTTGGCTATCAGCGCCGGGCCTCAGATGATGGCAA
  GCTCACGGATTCTTCTAAGACAAGCAATACTATCCGGGTTTTCTTGCCGAATAAGCAAAGGACTGTGGTC
  AATGTGCGGAATGGAATGAGCTTACATGACTGCCTTATGAAAGCTCTGAAGGTGAGAGGCCTGCAGCCAG
  AGTGCTGTGCAGTGTTCAGACTTCTCCAGGAACACAAAGGTAAGAAAGCACGCTTAGATTGGAACACCGA
  TGCCGCCTCTCTGATTGGAGAAGAACTGCAAGTGGATTTTTTGGATCATGTTCCACTCACAACTCACAAC
  TTTGCTCGGAAAACGTTCCTGAAGCTTGCATTCTGTGACATCTGTCAGAAGTTCCTGCTAAATGGATTTC
  GATGTCAGACTTGTGGCTACAAGTTTCATGAGCACTGTAGCACCAAAGTACCTACTATGTGTGTGGACTG
  GAGTAATATCAGACAGCTCTTGCTGTTTCCAAATTCCACTGTTGGTGACAGTGGAGTCCCAGCACCACCT
  TCTTTCCCAATGCGTCGGATGCGAGAATCTGTTTCCCGGATGCCTGCTAGTTCCCAGCACAGATACTCTA
  CACCCCATGCCTTCACTTTCAACACCTCCAGCCCTTCCTCAGAAGGTTCCCTGTCCCAGAGGCAGAGGTC
  AACGTCCACTCCCAATGTCCACATGGTCAGCACCACCCTGCATGTGGACAGCAGGATGATTGAGGATGCA
  ATTCGAAGTCACAGTGAATCAGCCTCACCTTCAGCCCTGTCCAGCAGCCCAAACAACCTGAGTCCAACAG
  GCTGGTCACAGCCCAAAACCCCTGTGCCAGCACAAAGAGAGCGGGCACCAGGATCTGGGACCCAGGAAAA
  AAACAAAATTAGGCCTCGTGGGCAGAGAGACTCGAGTTATTACTGGGAAATAGAAGCCAGTGAGGTGATG
  CTGTCTACTCGGATCGGGTCAGGTTCCTTTGGCACTGTGTACAAGGGCAAGTGGCATGGAGATGTTGCAG
  TAAAGATCCTAAAGGTGGTTGACCCAACTCCAGAGCAACTTCAGGCCTTCAGGAACGAGGTGGCTGTTTT
  GCGCAAAACACGGCATGTTAACATCCTGCTGTTCATGGGGTACATGACAAAGGACAACCTGGCGATTGTG
  ACTCAGTGGTGTGAAGGCAGCAGTCTCTACAAACACCTGCATGTCCAGGAGACCAAATTCCAGATGTTCC
  AGCTAATTGACATTGCCCGACAGACAGCTCAGGGAATGGACTATTTGCATGCAAAGAACATCATCCACAG
  AGACATGAAATCCAACAATATATTTCTCCATGAAGGCCTCACGGTGAAAATTGGAGATTTTGGTTTGGCA
  ACAGTGAAGTCACGCTGGAGTGGTTCTCAGCAGGTTGAACAGCCCACTGGCTCTGTGCTGTGGATGGCCC
  CAGAAGTAATCCGGATGCAGGATGACAACCCGTTCAGCTTCCAGTCCGACGTGTACTCGTACGGCATCGT
  GCTGTACGAGCTGATGGCTGGGGAGCTTCCCTACGCCCACATCAACAACCGAGACCAGATCATCTTCATG
  GTAGGCCGTGGGTATGCATCCCCTGATCTCAGCAGGCTCTACAAGAACTGCCCCAAGGCAATGAAGAGGT
  TGGTGGCTGACTGTGTGAAGAAAGTCAAAGAAGAGAGACCTTTGTTTCCCCAGATCCTGTCTTCCATCGA
  GCTGCTTCAGCACTCTCTGCCGAAAATCAACAGGAGCGCCTCTGAGCCTTCCCTGCATCGGGCAGCTCAC
  ACTGAGGACATCAATGCTTGCACGCTGACTACATCCCCAAGGCTACCAGTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209737 representing NM_029780
  Red=Cloning site Green=Tags(s)
  
  MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVV
  NVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHN
  FARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDSGVPAPP
  SFPMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLHVDSRMIEDA
  IRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVM
  LSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIV
  TQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLA
  TVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPYAHINNRDQIIFM
  VGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAH
  TEDINACTLTTSPRLPVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029780
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029780.4
• RefSeq Size: 3067 bp
• RefSeq ORF: 1947 bp
• Locus ID: 110157
• UniProt ID: Q99N57
• Cytogenetics: 6 53.62 cM
• MW: 73.4 kDa
• Gene Summary: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation (By similarity). Regulates Rho signaling and migration, and is required for normal wound healing.[UniProtKB/Swiss-Prot Function]