Plat (NM_008872) Mouse Tagged ORF Clone

CAT#: MR208868

Plat (Myc-DDK-tagged) - Mouse plasminogen activator, tissue (Plat)

Product Images

Circular map for MR208868

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plat
• Synonyms: AU020998; AW212668; D8Ertd2; D8Ertd2e; t; t-; tPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208868 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGAGAGCTGCTGTGTGTACTGCTGCTTTGTGGACTGGCTTTCCCATTGCCTGACCAGGGAATAC
  ATGGGAGGTTCAGAAGAGGAGCCCGGTCCTACAGAGCGACCTGCAGAGATGAGCCAACGCAGACAACTTA
  CCAACAGCACCAGTCGTGGCTCCGACCCATGCTCAGAAGCAGCCGGGTGGAATATTGCCGGTGCAACAGC
  GGCCTGGTACAATGCCACTCAGTGCCTGTCCGAAGTTGCAGCGAACCAAGATGCTTCAATGGGGGGACGT
  GTCAGCAGGCCCTGTATTTCTCTGACTTTGTCTGCCAGTGCCCTGATGGATTTGTAGGGAAACGCTGTGA
  CATAGATACCAGAGCAACATGCTTTGAGGAGCAGGGCATCACCTACAGAGGCACGTGGAGCACAGCAGAA
  AGTGGGGCTGAGTGCATCAACTGGAATAGCAGTGTTCTGTCGCTGAAGCCCTACAATGCAAGGAGGCCAA
  ATGCCATCAAGCTGGGCCTTGGGAATCACAATTACTGCAGAAACCCAGACCGAGACTTGAAGCCCTGGTG
  CTATGTCTTTAAGGCAGGGAAGTATACCACGGAGTTCTGTAGCACACCAGCTTGCCCTAAGGGAAAAAGC
  GAGGACTGCTATGTTGGGAAAGGTGTGACTTACCGTGGCACCCACAGCCTCACCACATCCCAGGCCTCCT
  GCCTCCCCTGGAATTCCATAGTCCTCATGGGCAAGAGTTACACAGCGTGGAGGACCAACTCCCAGGCACT
  CGGCCTGGGCAGACACAATTATTGTCGGAATCCAGATGGTGATGCCAGACCTTGGTGCCATGTGATGAAG
  GACCGAAAGCTGACGTGGGAATACTGTGACATGTCCCCATGCTCCACCTGTGGCCTGAGGCAGTACAAAC
  GGCCTCAGTTTAGAATTAAAGGAGGACTCTACACAGACATCACCTCACACCCTTGGCAGGCTCCCATCTT
  TGTCAAGAACAAGAGGTCTCCTGGAGAGAGATTCCTTTGTGGAGGGGTGCTGATCAGTTCCTGCTGGGTG
  CTGTCAGCTGCCCACTGCTTTCTAGAGAGGTTCCCCCCCAATCATCTTAAAGTGGTCTTGGGCAGAACAT
  ACAGGGTGGTCCCCGGAGAGGAAGAACAGACATTTGAGATTGAAAAATACATAGTCCATGAGGAATTTGA
  TGACGATACTTATGACAACGACATCGCATTACTGCAGCTGAGGTCACAGTCCAAGCAATGTGCCCAAGAG
  AGCAGCTCTGTTGGCACTGCCTGCCTCCCTGACCCCAACCTGCAGCTCCCTGACTGGACAGAGTGTGAGC
  TTTCTGGCTACGGCAAGCATGAGGCATCGTCTCCATTCTTCTCTGACCGGCTGAAGGAGGCTCACGTCAG
  ACTGTACCCGTCCAGCCGCTGCACCTCACAGCATCTGTTTAATAAAACCGTCACGAACAACATGCTGTGC
  GCTGGAGACACCCGGAGCGGAGGCAACCAAGACCTCCACGATGCATGCCAGGGTGACTCGGGAGGCCCTC
  TGGTGTGCATGATCAATAAACAGATGACATTGACTGGCATTATCAGCTGGGGCCTCGGCTGTGGGCAGAA
  GGATGTGCCTGGGGTCTACACAAAGGTTACGAATTACCTAGACTGGATTCACGACAACATGAAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208868 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRELLCVLLLCGLAFPLPDQGIHGRFRRGARSYRATCRDEPTQTTYQQHQSWLRPMLRSSRVEYCRCNS
  GLVQCHSVPVRSCSEPRCFNGGTCQQALYFSDFVCQCPDGFVGKRCDIDTRATCFEEQGITYRGTWSTAE
  SGAECINWNSSVLSLKPYNARRPNAIKLGLGNHNYCRNPDRDLKPWCYVFKAGKYTTEFCSTPACPKGKS
  EDCYVGKGVTYRGTHSLTTSQASCLPWNSIVLMGKSYTAWRTNSQALGLGRHNYCRNPDGDARPWCHVMK
  DRKLTWEYCDMSPCSTCGLRQYKRPQFRIKGGLYTDITSHPWQAPIFVKNKRSPGERFLCGGVLISSCWV
  LSAAHCFLERFPPNHLKVVLGRTYRVVPGEEEQTFEIEKYIVHEEFDDDTYDNDIALLQLRSQSKQCAQE
  SSSVGTACLPDPNLQLPDWTECELSGYGKHEASSPFFSDRLKEAHVRLYPSSRCTSQHLFNKTVTNNMLC
  AGDTRSGGNQDLHDACQGDSGGPLVCMINKQMTLTGIISWGLGCGQKDVPGVYTKVTNYLDWIHDNMKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008872
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008872.1, NM_008872.2, NM_008872.3, NP_032898.1
• RefSeq Size: 2548 bp
• RefSeq ORF: 1680 bp
• Locus ID: 18791
• UniProt ID: P11214
• Cytogenetics: 8 11.42 cM
• MW: 63.1 kDa
• Gene Summary: This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation. [provided by RefSeq, Jul 2015]