Sufu (NM_001025391) Mouse Tagged ORF Clone

CAT#: MR207758

Sufu (Myc-DDK-tagged) - Mouse suppressor of fused homolog (Drosophila) (Sufu), transcript variant 2

Product Images

Circular map for MR207758

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sufu
• Synonyms: Su(fu)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207758 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGCGGCCTAGCGTCGCCCCCGGTCCCGCCGCGCCCCCGGCCTCTGGCCCTAGTGCCCCTC
  CGGCCTTTGCTTCACTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAGTGTCGCCGCCTCTACCCTGA
  CCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTACTGGTTGGGTGGTCCGGACCCCTTGGACTAT
  GTTAGCATGTACAGGAACATGGGGAGTCCTTCTGCCAACATCCCTGAGCACTGGCACTACATCAGCTTTG
  GCCTGAGTGATCTCTATGGTGACAACAGAGTCCATGAGTTTACAGGAACAGACGGACCAAGTGGATTTGG
  CTTTGAGTTGACGTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCAGCAGAGCTG
  ATGCAGGGCCTAGCCCGATATGTCTTCCAGTCAGAGAACACCTTCTGTAGCGGGGACCATGTGTCTTGGC
  ACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACGGAGGACCCACAGATGCAGCC
  TGTGCGGACACCCTTTGGGGTAGTGACTTTCCTCCAGATTGTTGGTGTCTGCACTGAGGAGTTACATTCA
  GCCCAACAGTGGAACGGGCAGGGCATCCTGGAACTACTACGGACAGTGCCCATTGCTGGCGGTCCCTGGC
  TGATAACTGACATGCGGCGGGGAGAAACCATATTTGAGATCGATCCGCACCTGCAAGAGAGAGTTGACAA
  AGGCATTGAGACAGACGGTTCTAACCTGAGCGGCGTCAGTGCCAAGTGTGCCTGGGATGACCTCAGCCGG
  CCTCCGGAGGATGAAGAGGATAGCCGGAGCATCTGCCTCGGCACACAGCCTCGGAGGCTGTCTGGCAAAG
  ACACAGAGCAGATCCGGGAGACCCTGAGGCGGGGACTGGAGATTAACAGCAAACCTGTCCTTCCACCAAT
  CAATTCTCAGCGACAGAACGGCCTCACCCACGACAGGGCTCCGAGCCGCAAGGACAGTTTGGGCAGCGAC
  AGCTCCACGGCCATCATCCCCCACGAGCTGATCCGCACACGGCAGCTCGAGAGCGTGCATCTAAAATTTA
  ACCAAGAGTCGGGAGCCCTCATCCCTCTCTGCCTAAGGGGCAGACTCCTACATGGCCGGCACTTCACCTA
  CAAGAGTATCACAGGCGACATGGCCATCACGTTTGTGTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG
  GAACACCCGTATGCAGCTCACGGACCCTGGTTACAAATTCTGTTGACAGAAGAGTTTGTAGAGAAGATGT
  TGGAGGACTTAGAAGATCTAACCTCTCCAGAGGAATTTAAACTTCCCAAAGAGTACAGCTGGCCTGAGAA
  GAAACTCAAAGTGTCCATTCTCCCCGACGTGGTGTTCGACAGTCCACTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207758 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAELRPSVAPGPAAPPASGPSAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDY
  VSMYRNMGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAEL
  MQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVRTPFGVVTFLQIVGVCTEELHS
  AQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSR
  PPEDEEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTHDRAPSRKDSLGSD
  SSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATE
  EHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025391
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025391.2, NP_001020562.1
• RefSeq Size: 4522 bp
• RefSeq ORF: 1455 bp
• Locus ID: 24069
• UniProt ID: Q9Z0P7
• Cytogenetics: 19 38.85 cM
• MW: 54 kDa
• Gene Summary: Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:16155214, PubMed:16459298). Down-regulates GLI1-mediated transactivation of target genes (PubMed:11960000). Part of a corepressor complex that acts on DNA-bound GLI1 (PubMed:11960000). May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (By similarity). Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10531011, PubMed:16459298). Negative regulator of beta-catenin signaling (PubMed:11477086). Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL) (PubMed:10531011, PubMed:20360384). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state (PubMed:10531011, PubMed:20360384). Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R (PubMed:10531011, PubMed:20360384). When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) (PubMed:10531011, PubMed:20360384). Required for normal embryonic development (PubMed:16155214, PubMed:16459298). Required for the proper formation of hair follicles and the control of epidermal differentiation (PubMed:16155214, PubMed:16459298, PubMed:23034632).[UniProtKB/Swiss-Prot Function]