Cdx1 (NM_009880) Mouse Tagged ORF Clone

CAT#: MR203563

Cdx1 (Myc-DDK-tagged) - Mouse caudal type homeobox 1 (Cdx1)

Product Images

Circular map for MR203563

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdx1
• Synonyms: Cdx; Cdx-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203563 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGTGGGCTATGTGCTGGACAAGGACTCCCCCGTGTACCAAGGCCCCGCCAGACCCTCCAGCCTCG
  GCCTGGGCCCTCCGACCTATGCGCCCCCTGGCCCGGCGCCCGCGCCCCCGCAGTACCCCGACTTCGCGGG
  TTACACGCACGTGGAGCCGGCGCCCGCGCCCCCTCCGACCTGGGCTGCGCCCTTCCCTGCGCCCAAGGAC
  GACTGGGCAGCTGCCTATGGCCCGGGCCCCACGGCCTCAGCCGCCAGCCCGGCCCCGCTGGCCTTCGGGC
  CCCCTCCGGACTTTAGCCCGGTGCCCGCGCCTCCCGGGCCTGGTCCCGGCATCCTGGCGCAGTCCCTCGG
  GGCTCCGGGCGCACCGTCCTCGCCAGGAGCGCCAAGGCGGACGCCCTACGAATGGATGCGGCGCAGCGTG
  GCGGCTGCAGGCGGCGGTGGCAGCGGTAAGACCCGAACCAAGGACAAGTACCGTGTGGTCTACACAGACC
  ACCAACGCCTAGAGCTGGAAAAGGAGTTTCACTACAGCCGGTACATCACTATCCGGCGCAAGTCCGAGCT
  GGCTGCTAACCTGGGGCTCACAGAGCGGCAGGTAAAGATCTGGTTCCAGAACCGCCGGGCCAAGGAGCGC
  AAAGTAAACAAGAAGAAACAGCAGCAGCAGCAGCCCCTGCCTCCCACACAGCTGCCCCTGCCCCTGGATG
  GCACTCCCACACCATCAGGGCCACCCCTAGGAAGTCTATGCCCTACTAATGCTGGCCTTCTGGGCACCCC
  CTCCCCAGTGCCCGTCAAGGAGGAGTTTCTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203563 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYVGYVLDKDSPVYQGPARPSSLGLGPPTYAPPGPAPAPPQYPDFAGYTHVEPAPAPPPTWAAPFPAPKD
  DWAAAYGPGPTASAASPAPLAFGPPPDFSPVPAPPGPGPGILAQSLGAPGAPSSPGAPRRTPYEWMRRSV
  AAAGGGGSGKTRTKDKYRVVYTDHQRLELEKEFHYSRYITIRRKSELAANLGLTERQVKIWFQNRRAKER
  KVNKKKQQQQQPLPPTQLPLPLDGTPTPSGPPLGSLCPTNAGLLGTPSPVPVKEEFLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009880
• ORF Size: 807 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009880.2, NP_034010.2
• RefSeq Size: 1750 bp
• RefSeq ORF: 807 bp
• Locus ID: 12590
• UniProt ID: P18111
• Cytogenetics: 18 34.41 cM
• MW: 28.5 kDa
• Gene Summary: Plays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA.[UniProtKB/Swiss-Prot Function]