Tjp1 (NM_009386) Mouse Tagged ORF Clone

CAT#: MG227561

Tjp1 (tGFP-tagged) - Mouse tight junction protein 1 (Tjp1) transcript variant 1, (10ug)

Product Images

Circular map for MG227561

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tjp1
• Synonyms: ZO1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227561 representing NM_009386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG
  TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT
  TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG
  GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC
  AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG
  TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA
  GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG
  AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT
  CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA
  ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG
  GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA
  AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT
  GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC
  ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC
  GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG
  GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA
  AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT
  GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC
  AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG
  GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT
  TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC
  CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG
  ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT
  TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT
  CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG
  CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT
  TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC
  CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG
  CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG
  AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG
  GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA
  TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC
  TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA
  ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC
  AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA
  CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT
  TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG
  ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG
  AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA
  GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCCTCATCTCCAGTCCCTTACCTTTCGC
  CTGAAACAACCCCAGCATCATCAGCCTCTGCAGTTAATCATAATGTCAGTGTAACTAATGTCAGCCTGGA
  GGAGCCTGCCCCAGCCCCTCCCACCTCGCACGCATCACAGCCTGGTTGTTTAGGAGCACCAAGTGCTGAG
  GCAGCTCACGTAGTGCTCAGAGGTGAAGGACCACCATTGCCGCCGCATGCAGACCCAGCAAAGGTGTACA
  GGAAGGAGCCGTATTCTGAAGAAATGATGAGACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCC
  AGGGCAGAGGCCAGATAAAGAGCCAAATCTAGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCC
  AGCAGAGACCTTGAGCAGCCGTCATACAGGTATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACT
  ATGACCATCGCCTACGGTTTGAAGATCGAATCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAA
  ACAGCCCTACCAACCTCGGCCTTTTGAGAATCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAA
  GAGGCTTCAGAACGAGGTTATTTCCAGCGTTTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACAC
  GCTATGAGCAGCTGCCTCGAACCTCTACTCTACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCA
  CAACAGGTACAGGCCAGAGGCACAGCCCTATTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTAC
  TTTGACCAGTACCCGCGAAGTTATGAGCAAGTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACG
  AGCCTCTCCATGGTGCTGCAGTTGTCCCTCCTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCC
  CTCGGCTACCAAACCACAGCCTCCACCCCCAACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCA
  CAGTCTGTGCTCACCAGAGTCAAAATGTTTGAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATG
  TGAATGACACTGCCAGCTTTAAGCCTCCGGAAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCC
  TAAACCTGTCCCTCAGAGTCAGTTTAGTGAGCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAA
  CCTCAAGTGAAGCCACCCGAAGATATTGTTCGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATT
  ACCGGAAACAGCTCTCCTACTTTGACCGAAGAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGG
  CCATCACTCAGAGCCTGCTAAGCCAGTCCATTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAG
  GGAAAACCCGAAACTGATGCTGTGGATAGATCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGC
  CTCCTCCTCCTCCGTTGCCCTCACAGTACAGCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACAT
  ACATTCCAAGGGCGCCCAGGGTGAAGGCAACTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAA
  CCAGACCCACCCCCATCTCAGAGTAAACCAGCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGA
  CTTTCTATCCGCAGAAAAGTTTCCCAGACAAAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCAT
  CACTCCGGTGTACAATCGATTCACACCAAAGCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTT
  GAAAGTCCGAAGTTCAACCATAATCTTCTGCCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAA
  CTCCCACTTCCCCAAAAACTCTTATGAAAGCTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGT
  TGAAACTTTCTCTGTTCACACAGATAAGCCTAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCT
  GTCCCTGTGAGTCCTTCAGCTGTGGAAGAAGATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTC
  GTGGCATTTTTAACAGCAATGGTGGTGTGTTGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACA
  AGGAGCCATTCCTGAAGGAATTGAGCAAGAAATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCA
  CCTTTAGATAAAGAGAAAGGTGAAACTCTGCTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGT
  TCCTGAAGCCCGTGGAGCTACGCTTGCCACACTGTGACCCTAAAACCTGGCAAAACAAGTGTCTTCCTGG
  AGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTCCTGATTGACCACTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227561 representing NM_009386
  Red=Cloning site Green=Tags(s)
  
  MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQ
  ENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPR
  AGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKE
  ISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHS
  ANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPG
  AISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRP
  SMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDL
  PKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAI
  RIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKF
  PAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQ
  DKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFT
  TTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHT
  SDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRI
  DSPGLKPASQQKAEASSPVPYLSPETTPASSASAVNHNVSVTNVSLEEPAPAPPTSHASQPGCLGAPSAE
  AAHVVLRGEGPPLPPHADPAKVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQA
  SRDLEQPSYRYEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPE
  EASERGYFQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQY
  FDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKP
  QSVLTRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQK
  PQVKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSK
  GKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSK
  PDPPPSQSKPATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKF
  ESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKA
  VPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILP
  PLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009386
• ORF Size: 5235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009386.2, NP_033412.2
• RefSeq Size: 7054 bp
• RefSeq ORF: 5238 bp
• Locus ID: 21872
• UniProt ID: P39447
• Cytogenetics: 7 35.02 cM
• Gene Summary: Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]