Tap1 (NM_001161730) Mouse Tagged ORF Clone

CAT#: MG226877

Tap1 (tGFP-tagged) - Mouse transporter 1 ATP-binding cassette sub-family B (MDR/TAP) (Tap1) transcript variant 2, (10ug)

Product Images

Circular map for MG226877

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tap1
• Synonyms: ABC17; Abcb; Abcb2; APT1; Ham; Ham-; Ham-1; Ham1; MTP; MTP1; PSF; PSF1; RI; RING4; T; TAP; Tap-1; Y3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226877 representing NM_001161730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCACGTCTGGCTGGCGGCCGCCCTGCTCCTTCTGGTGGACTGGCTGCTGCTGCGGCCCATGC
  TCCCGGGAATCTTCTCCCTGTTGGTTCCCGAGGTGCCGCTGCTCCGGGTCTGGGTGGTGGGCCTGAGTCG
  CTGGGCCATCCTAGGACTAGGGGTCCGCGGGGTCCTCGGGGTCACCGCAGGAGCCCATGGCTGGCTGGCT
  GCTTTGCAGCCGCTGGTGGCCGCACTGAGTTTGGCCCTGCCTGGACTTGCCTTGTTCCGAGAGCTGGCCG
  CCTGGGGAACACTCCGGGAGGGTGACAGCGCTGGATTACTGTACTGGAACAGTCGTCCAGATGCCTTCGC
  TATCAGTTATGTGGCAGCATTGCCCGCAGCCGCCCTGTGGCACAAGTTGGGGAGCCTCTGGGCGCCCAGC
  GGCAACAGGGACGCTGGAGACATGCTGTGTCGGATGCTGGGCTTCCTGGGCCCTAAGAAGAGACGTCTCT
  ACCTGGTTCTGGTTCTCTTGATTCTCTCTTGCCTTGGGGAAATGGCCATTCCCTTCTTCACGGGCCGCAT
  CACTGACTGGATTCTTCAGGATAAGACAGTTCCTAGCTTCACCCGCAACATATGGCTCATGTCCATTCTC
  ACCATAGCCAGCACAGCGCTGGAGTTTGCAAGTGATGGAATCTACAACATCACCATGGGACACATGCACG
  GCCGTGTGCACAGAGAGGTGTTTCGGGCCGTCCTTCGCCAGGAGACAGGGTTTTTCCTGAAGAACCCAGC
  AGGTTCCATCACATCTCGGGTGACTGAGGACACAGCCAACGTGTGCGAGTCCATTAGTGGCACGCTGAGC
  CTGCTGCTGTGGTACCTGGGGCGAGCCCTGTGTCTCTTGGTGTTCATGTTTTGGGGGTCACCGTACCTCA
  CTCTGGTCACCCTGATCAACCTGCCCCTGCTTTTTCTTTTGCCTAAGAAGCTGGGAAAAGTGCATCAGTC
  ACTGGCAGTGAAGGTGCAGGAGTCTCTAGCAAAGTCCACGCAGGTGGCCCTTGAGGCCTTATCGGCGATG
  CCTACTGTGCGGAGCTTTGCCAACGAGGAGGGTGAGGCCCAGAAGTTCAGGCAGAAGTTGGAAGAAATGA
  AGACTCTAAACAAGAAGGAGGCCTTGGCTTACGTGGCTGAAGTCTGGACCACGAGTGTCTCGGGAATGCT
  GCTGAAGCTTCAGTTCACCCAGGCTGTTCAGGTCCTGCTCTCCCTCTACCCCTCCATGCAGAAGGCTGTG
  GGCTCCTCAGAGAAAATATTCGAATACTTGGACCGGACTCCTTGCTCTCCACTCAGTGGCTCGTTGGCAC
  CCTCAAACATGAAAGGCCTTGTGGAGTTCCAAGATGTCTCTTTTGCCTACCCAAACCAGCCCAAAGTCCA
  GGTGCTTCAGGGGCTGACGTTCACCCTGCATCCTGGAACGGTGACAGCGTTGGTGGGACCCAATGGATCA
  GGGAAGAGCACCGTGGCTGCCCTGCTGCAGAACCTGTACCAGCCCACCGGGGGCCAGCTGCTGCTGGATG
  GCCAGTGCCTGGTCCAGTATGATCACCATTACCTGCACACTCAGGTGGCCGCAGTGGGACAAGAGCCGCT
  GCTATTTGGAAGAAGCTTTCGAGAAAATATTGCGTATGGCCTGAACCGGACTCCAACCATGGAGGAAATC
  ACAGCTGTGGCCGTGGAGTCTGGAGCCCACGATTTCATCTCTGGGTTCCCTCAGGGCTATGACACAGAGG
  TAGGTGAGACTGGGAACCAGCTGTCAGGAGGTCAGCGACAGGCAGTGGCCTTGGCCCGAGCCTTGATCCG
  GAAGCCACTCCTGCTTATCTTGGATGATGCCACCAGTGCCCTGGATGCTGGCAACCAGCTACGGGTCCAG
  CGGCTCCTGTATGAGAGCCCCAAGCGGGCTTCTCGGACGGTTCTTCTTATCACCCAGCAGCTCAGCCTGG
  CAGAGCAGGCCCACCACATCCTCTTTCTCAGAGAAGGCTCTGTCGGCGAGCAGGGCACCCACCTGCAGCT
  CATGAAGAGAGGAGGGTGCTACCGGGCCATGGTAGAGGCTCTTGCGGCTCCTGCAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226877 representing NM_001161730
  Red=Cloning site Green=Tags(s)
  
  MAAHVWLAAALLLLVDWLLLRPMLPGIFSLLVPEVPLLRVWVVGLSRWAILGLGVRGVLGVTAGAHGWLA
  ALQPLVAALSLALPGLALFRELAAWGTLREGDSAGLLYWNSRPDAFAISYVAALPAAALWHKLGSLWAPS
  GNRDAGDMLCRMLGFLGPKKRRLYLVLVLLILSCLGEMAIPFFTGRITDWILQDKTVPSFTRNIWLMSIL
  TIASTALEFASDGIYNITMGHMHGRVHREVFRAVLRQETGFFLKNPAGSITSRVTEDTANVCESISGTLS
  LLLWYLGRALCLLVFMFWGSPYLTLVTLINLPLLFLLPKKLGKVHQSLAVKVQESLAKSTQVALEALSAM
  PTVRSFANEEGEAQKFRQKLEEMKTLNKKEALAYVAEVWTTSVSGMLLKLQFTQAVQVLLSLYPSMQKAV
  GSSEKIFEYLDRTPCSPLSGSLAPSNMKGLVEFQDVSFAYPNQPKVQVLQGLTFTLHPGTVTALVGPNGS
  GKSTVAALLQNLYQPTGGQLLLDGQCLVQYDHHYLHTQVAAVGQEPLLFGRSFRENIAYGLNRTPTMEEI
  TAVAVESGAHDFISGFPQGYDTEVGETGNQLSGGQRQAVALARALIRKPLLLILDDATSALDAGNQLRVQ
  RLLYESPKRASRTVLLITQQLSLAEQAHHILFLREGSVGEQGTHLQLMKRGGCYRAMVEALAAPAD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161730
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161730.1, NP_001155202.1
• RefSeq Size: 2866 bp
• RefSeq ORF: 2091 bp
• Locus ID: 21354
• Cytogenetics: 17 17.98 cM
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]