Dcc (NM_007831) Mouse Tagged ORF Clone

CAT#: MG226661

Dcc (tGFP-tagged) - Mouse deleted in colorectal carcinoma (Dcc), (10ug)

Product Images

Circular map for MG226661

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dcc
• Synonyms: C030036D22Rik; Igdcc1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226661 representing NM_007831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATAGTCTTGGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCA
  GCGCACATCTTCAAGTCACGGGTTTCCAAATTAAACCTTTCACGTCACTTCATTTTGTGTCTGAACCTTC
  GGATGCTGTCACCATGCGGGGAGGAAATGTCCTGCTCAACTGCTCTGCAGAGTCCGACCGAGGAGTTCCA
  GTCATCAAGTGGAAGAAAGACGGCCTTATCCTAGCCTTGGGCATGGATGACAGAAAGCAGCAACTTCCTA
  ATGGATCTCTGTTGATACAAAACATACTTCATTCCAGACACCATAAGCCGGATGAAGGACTTTACCAATG
  CGAGGCATCCTTAGCAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTACGGTAGCAGGGCCATTG
  AGGTTCCTTTCCCAGACGGAATCTATCACAGCCTTCATGGGAGATACAGTGTTACTTAAGTGTGAAGTCA
  TTGGGGAACCCATGCCAACAATACACTGGCAGAAAAATCAACAAGACCTCAACCCACTCCCAGGAGACTC
  GCGTGTGGTCGTCTTGCCCTCCGGAGCATTGCAGATTAGCCGACTTCAGCCAGGAGACAGTGGAGTCTAC
  AGATGCTCGGCTCGGAATCCAGCCAGCATAAGAACAGGAAATGAAGCAGAAGTTAGAATTTTATCAGACC
  CAGGCCTGCATAGGCAGCTATATTTTCTGCAGAGACCATCCAACGTAATAGCCATTGAAGGGAAAGATGC
  TGTCCTGGAGTGCTGTGTTTCTGGCTATCCTCCGCCAAGTTTCACCTGGTTACGAGGCGAGGAGGTCATC
  CAACTCAGGTCTAAAAAGTATTCATTGTTGGGTGGAAGCAATTTGCTCATCTCTAATGTGACAGATGATG
  ACAGTGGAACCTATACTTGCGTTGTCACCTATAAGAATGAGAATATTAGTGCCTCTGCAGAGCTCACGGT
  CTTGGTTCCGCCCTGGTTTTTAAATCACCCTTCCAACCTCTATGCCTATGAGAGCATGGATATTGAGTTT
  GAATGTGCAGTTTCTGGGAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTGATCCCGA
  GTGACTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATCCTTGGGGTGGTCAAGTCAGATGAAGGCTT
  CTATCAATGTGTGGCTGAAAATGAGGCTGGCAATGCCCAAAGTAGTGCCCAGCTGATTGTCCCCAAGCCT
  GCCATCCCAAGCTCCAGCATCCTCCCTTCTGCTCCCAGAGATGTGCTCCCTGTCTTGGTTTCTAGTCGCT
  TTGTCCGTTTGAGCTGGCGTCCACCTGCAGAAGCAAAAGGGAACATTCAAACCTTCACAGTCTTTTTCTC
  CAGAGAGGGTGACAACAGGGAGCGAGCTTTGAATACAACCCAGCCTGGGTCCCTTCAGCTTACTGTGGGA
  AACTTGAAACCAGAAGCCATGTACACCTTCAGAGTTGTCGCCTACAATGAGTGGGGACCAGGGGAGAGTT
  CTCAGCCCATCAAGGTGGCCACTCAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCATGCTGT
  ATCTACCTCACCTACCTCAATTCTTATTACATGGGAACCCCCTGCCTATGCAAATGGCCCAGTCCAAGGT
  TACAGATTGTTCTGCACTGAGGTGTCTACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATA
  AACTGGAAGGCCTGAAAAAATTCACGGAATATACTCTTCGATTCCTAGCTTATAATCGTTATGGGCCAGG
  AGTATCTACTGATGATATAACAGTTGTTACACTTTCAGACGTGCCAAGTGCTCCACCTCAGAACATCTCA
  CTGGAAGTGGTTAACTCAAGGAGTATCAAAGTAAGCTGGCTCCCTCCTCCATCGGGAACACAAAATGGAT
  TTATTACTGGCTATAAAATTCGACACAGAAAGACTACCCGCAGGGGCGAGATGGAAACACTGGAACCGAA
  TAACCTCTGGTACCTCTTCACAGGATTGGAGAAAGGCAGTCAGTACAGTTTCCAAGTGTCGGCCATGACA
  GTCAATGGTACTGGACCACCTTCCAACTGGTACACAGCGGAGACCCCAGAGAATGATTTAGATGAGTCTC
  AAGTCCCTGACCAGCCGAGTTCTCTTCACGTGAGGCCCCAGACAAACTGTATCATCATGAGTTGGACACC
  TCCTCTCAACCCAAACATCGTGGTGAGAGGCTATATTATTGGCTATGGTGTTGGCAGTCCTTATGCTGAA
  ACAGTGCGTGTGGACAGTAAGCAGCGGTACTATTCCATCGAGAGACTAGAGTCGAGTTCTCATTATGTAA
  TCTCCTTAAAAGCTTTCAACAATGCCGGAGAAGGTGTCCCTCTCTACGAAAGTGCCACCACTCGTTCCAT
  AACAGATCCCACTGACCCCGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGGCCCAGATGTC
  TCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGAGGCTGTGAGGGTCAGCT
  GGGCAGACAACTCTGTCCCGAAGAACCAGAAAACATCCGATGTGCGACTGTACACCGTTCGGTGGAGGAC
  CAGCTTTTCTGCCAGTGCCAAGTACAAGTCAGAAGACACAACATCTCTCAGTTACACAGCGACTGGCCTC
  AAGCCCAATACCATGTACGAGTTCTCAGTCATGGTGACCAAGAACAGAAGGTCAAGCACGTGGAGCATGA
  CTGCACACGCCACCACCTACGAAGCTGCCCCAACTTCTGCTCCCAAGGATCTGACAGTCATTACTCGAGA
  GGGGAAGCCTCGTGCTGTCATTGTGAGCTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATCACTGCTTAT
  ATCTTGTTTTATACATTGGACAAGAATATACCAATAGATGATTGGATTATGGAAACTATCAGCGGTGACC
  GGCTTACCCATCAGATCATGGATCTCAGCCTTGACACGATGTATTACTTTCGAATTCAAGCACGGAATGT
  GAAAGGAGTAGGGCCCCTCTCTGATCCCATCCTCTTCAGGACTTTGAAAGTGGAACACCCTGACAAAATG
  GCTAATGACCAAGGTCGTCATGGAGATGGGGGTTATTGGCCAGTTGATACTAACTTGATTGATAGAAGCA
  CTCTCAACGAACCACCCATTGGACAGATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAA
  CCTGCTTGTAATAACCGTGGTCACCGTTGGTGTTCTCACTGTGTTGGTCGTGGTGATTGTAGCAGTGATT
  TGCACCCGGCGTTCTTCAGCGCAGCAGAGGAAGAAACGGGCCACACACAGTGTGAGCAAGAGGAAGGGCA
  GTCAGAAGGACCTCCGGCCTCCCGATCTTTGGATACATCATGAAGAAATGGAAATGAAAAATATCGAGAA
  GCCTACGGGAACCGACCCTGCAGGAAGAGACTCCCCCATCCAGAGCTGCCAAGATCTCACACCAGTCAGC
  CATAGCCAGTCAGAAACCCAGATGGGAAGCAAAAGTGCCTCTCATTCAGGTCAGGACACTGAGGACGCAG
  GCAGCTCCATGTCCACTTTGGAACGGTCCCTGGCAGCACGCCGGGCCACCAGGGCCAAGCTCATGATTCC
  CATGGAGGCCCAGTCCAGTAATCCTGCTGTTGTGAGTGCCATCCCTGTACCAACACTAGAAAGTGCCCAG
  TATCCAGGAATCCTCCCATCTCCCACATGTGGATACCCGCATCCACAGTTCACTCTCCGGCCAGTACCAT
  TCCCAACGCTGTCTGTGGACCGAGGTTTTGGAGCAGGAAGAACTCAGTCTGTGAGTGAAGGACCAACCAC
  GCAACAGCAACCCATGCTGCCCCCAGCTCAGCCCGAACATCCGAGCAGTGAAGAAGCACCCAGTAGAACC
  ATCCCCACAGCCTGTGTGCGGCCAACTCATCCACTCCGCAGCTTTGCTAACCCATTACTACCTCCACCCA
  TGAGTGCAATAGAACCAAAAGTCCCTTACACACCGCTTTTGTCTCAGCCAGGACCCACCCTTCCCAAGAC
  TCATGTTAAAACGGCCTCCCTTGGGCTGGCCGGAAAGGCAAGGTCCCCTTTGCTTCCCGTGTCTGTGCCA
  ACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAGCCCACAGAGGATCCAGCCAGTGTATATGAACAGGATG
  ACTTGAGCGAACAAATGGCAAGTTTGGAAGGGCTAATGAAGCAACTCAACGCCATCACAGGGTCTGCCTT
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226661 representing NM_007831
  Red=Cloning site Green=Tags(s)
  
  MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVP
  VIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLADSGSIISRTAKVTVAGPL
  RFLSQTESITAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLNPLPGDSRVVVLPSGALQISRLQPGDSGVY
  RCSARNPASIRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVI
  QLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEF
  ECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKP
  AIPSSSILPSAPRDVLPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVG
  NLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSTSPTSILITWEPPAYANGPVQG
  YRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNIS
  LEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMT
  VNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAE
  TVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDV
  STPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGL
  KPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAY
  ILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNVKGVGPLSDPILFRTLKVEHPDKM
  ANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVI
  CTRRSSAQQRKKRATHSVSKRKGSQKDLRPPDLWIHHEEMEMKNIEKPTGTDPAGRDSPIQSCQDLTPVS
  HSQSETQMGSKSASHSGQDTEDAGSSMSTLERSLAARRATRAKLMIPMEAQSSNPAVVSAIPVPTLESAQ
  YPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTQSVSEGPTTQQQPMLPPAQPEHPSSEEAPSRT
  IPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVP
  TAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007831
• ORF Size: 4341 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007831.3, NP_031857.2
• RefSeq Size: 10325 bp
• RefSeq ORF: 4344 bp
• Locus ID: 13176
• UniProt ID: P70211
• Cytogenetics: 18 45.24 cM
• Gene Summary: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.[UniProtKB/Swiss-Prot Function]