Mov10 (NM_001163440) Mouse Tagged ORF Clone

CAT#: MG226505

Mov10 (tGFP-tagged) - Mouse Moloney leukemia virus 10 (Mov10) transcript variant 1, (10ug)

Product Images

Circular map for MG226505

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mov10
• Synonyms: C77703; Mov-10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226505 representing NM_001163440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGCTCTGCCCTTTGCTCTGCAGCTTTTCTATGTCCCGAGGCTCTGGAGAGTGGGCGTGGGAG
  TAGCCCTAGCTGAGGCTCCAAGGTCCTCCGGGCTGTTTAGGGGGCCCCAGGCGATATCCACGTTCGGATC
  CTGGCTCGCTGCCAGGGGAGGGAGCTCGGGTTTCATTTCCGCGGACCCTCCTGCCTGGGCCGCAGCCGCC
  GCCGCCGCGATGCCTAGCAAGTTCAGCTGCCGAAAGCTCCGGGAGACCGGCCAGAGGTTCGAGAGTTTTC
  TGGCCGAACGTGGACTGGACCTGGAGACAGATCGTGAGCGGCTGCGGACGATTTACAACCACGACTTCAA
  GCCCAGCTATGGGACCCCTGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATCGCAAATCTGGCC
  TTCGTCACCAAGACTCGGGTCAGGTTCTTCAAACTAGACCGCTGGGCTGATGTGCAGTTACCAGAAAAGA
  GGCGAATAAAGCCAGGGTCGAACATCAGCAAACAACACAGATCACTGTTGGCCAGGATCTTTCACGACAG
  GGCTGAGTACCTTCATGGGAAGCATGGGGTAGACGTGGAGGTCCAGGGGCCCCATGAAGCCCGAGACGGG
  CAACTCCTTATCCACCTGGATTTGAACCGCAAGGAGGTACTAACCCTACGGCTTCGGAACGGCGGAAGCA
  AACCTGTCACCCTCACTCACCTATTCCCACTGTGCTGGACGCCCCAGTTTGTCTTCTACCATGGAGAACA
  GGACCTGCCCTGCCCACTGGGCCCCGGTGAAAGCTATGAACTCCACATCTACTGTAAGACCAGCATTGTG
  GGTTACTTCCCAGCCACTGTCCTCTGGGAACTCCTGGGACCCGGGGAGTCGGGAGCAGAAGGAGCAGAAA
  CTTTCTACATTGCCCGATTCCTGGCGGCTGTCGCCCACAGTCCCCTGGCTGCCCAGTTGAAACCCACAAC
  TCCCTTCAAACGCCCCCCTCGGCTCACCAGAAACTCTGTGTTGACCAACCGGATCGAGGAAGGAGAGAGG
  CCCGACCGAGCCAAGGGCTATGAACTAGAGCTAAGTTTGGCCCTGGGGACCTATTACCCACCCATCCTCC
  TCCGACAACTGCTCCCTACCCTTCTTCAGGGACCAAGTATCTTCACTGCCCCAAAGGAGGTTGCTGAGAT
  CAAGGCCCAGCTGGAGACAACCCTGAAATCCAGGAACTATGAGGTGAAACTCCGGCTGCTGCTGCACCTG
  GAAGAGCTGCAGATGGAGCATGACATCCGGCACTATGACCTGGACTCGGTACCCATGACCTGGGACCCTG
  TGGACCAGAATCCCAGGCTGCTCACCCTGGAGGTTCCTGGTGTCGCAGAGAGCCGTCCCTCAGTGCTACG
  AGGTGACCACCTTTTTGCCCTTTTGTCCTCTGAGACCCAACAGGACGACCCTGTCACCTACAAGGGTTTC
  GTGCACAAGGTGGAACTGGACCGTGTCAAGCTGAGCTTTTCTACAAGCCTCCTGAGCCGATTTGTGGATG
  GGCTGACCTTCAAGGTGAACTTTACCTTCAACCGCCAGCCCCTTCGGGTCCAGCACCGGGCCCTAGAGTT
  GACGGGGCGCTGGGTGCTATGGCCCATGCTTTTTCCTGTGGCCTCCCGTGGGGTCTCGTTGCTGCCCTCA
  GATGTGAAGTTCAAGCTGTACGATCGGAGTCTGGAGTCAAACCCTGAGCAACTGCAGGCCATGAAGCACA
  TTGTGAGGGGTACCACCCGGCCTGCCCCCTACATCATCTTTGGGCCTCCAGGTACCGGCAAGACTGTCAC
  ATTAGTGGAGGCCATCAAACAGGTAGTGAAGCATTTGCCCAAAGCCCACATCCTGGCCTGTGCTCCATCC
  AACTCAGGGGCTGACCTCCTCTGTCAGCGGCTCCGGGTCCACCTGCCCAGCTCCATCTACCGTCTCCTGG
  CCCCCAGCAGGGACATCCGAATGGTGCCTGAGGACATTAAGACCTGCTGTAACTGGGATGCTAAGAAGGG
  AGAATATGTGTATCCTGCTAAGAAGCACCTGCAGCAATATCGGGTCTTAATTACCACCCTCATCACTGCC
  AGCAGGTTGGTGTCAGCCCAGTTTCCCATCGATCACTTCACACACATCTTCATCGATGAGGCTGGCCACT
  GCATGGAGCCTGAGAGTCTGGTGGCCATAGCAGGACTGATGGATGTCAAGGAAACGGGCAATCCCGGAGG
  GCAGCTGGTACTGGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGCTCGCCACTAGCCCTGAAGCAT
  GGACTTGGCTACTCTCTGCTAGAGCGCCTGCTCGCCTACAACTCCTTGTATAAGAAGGGCCCCAATGGCT
  ATGACCCCCAGTTCATCACCAAACTGCTCCGCAACTACAGGTCTCACCCCACCATCCTGGACATCCCTAA
  CCAGCTGTACTACGACGGAGAGCTGCAGGCCTGCGCAGATGTGGTGGATCGAGAACGGTTCTGCCGCTGG
  GAGGGGCTGCCTCAGCAGGGCTTCCCCATCATTTTCCATGGTGTAATGGGCAAAGATGAGAGAGAAGGCA
  ATAGCCCATCCTTCTTCAACCCTGAAGAGGCTGCTACAGTGACGTCATACCTGAAACAACTCCTGGCCCC
  TTCCTCCAAGAAGGGTAAAGCCCGCCTGAGCCCCCGAAATGTGGGCGTCATCTCCCCGTACCGGAAGCAG
  GTAGAAAAAATCCGTTACTGCATCACAAAACTTGACCGAGAACTTCGCGGACTGGATGACATCAAAGATT
  TGAAGGTGGGCTCTGTGGAAGAGTTCCAAGGCCAAGAACGCAGCGTCATCCTCATCTCCACCGTCCGAAG
  CAGCCAGAGCTTTGTACAGCTGGATCTAGACTTTAACCTCGGTTTCCTTAAGAACCCCAAGAGGTTCAAT
  GTTGCTGTGACCCGAGCCAAGGCTTTGCTCATCGTAGTGGGCAACCCCCTCCTCCTAGGCCACGACCCAG
  ACTGGAAAACGTTCCTGGAGTTCTGTAAAGAAAACGGGGGATATACCGGGTGCCCCTTTCCTGCCAAACT
  GGACCTGCAGCAGGGACAGGACTTGCTCCAAGGTCTGAGCAAACTCAGCCCCTCTACCTCAGGGCCCCGC
  CGTCACCAGAATCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGCCCTTACAAGTGGAGCCAGAGTGGA
  GAAATGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226505 representing NM_001163440
  Red=Cloning site Green=Tags(s)
  
  MSGALPFALQLFYVPRLWRVGVGVALAEAPRSSGLFRGPQAISTFGSWLAARGGSSGFISADPPAWAAAA
  AAAMPSKFSCRKLRETGQRFESFLAERGLDLETDRERLRTIYNHDFKPSYGTPAPGFSSMLYGMKIANLA
  FVTKTRVRFFKLDRWADVQLPEKRRIKPGSNISKQHRSLLARIFHDRAEYLHGKHGVDVEVQGPHEARDG
  QLLIHLDLNRKEVLTLRLRNGGSKPVTLTHLFPLCWTPQFVFYHGEQDLPCPLGPGESYELHIYCKTSIV
  GYFPATVLWELLGPGESGAEGAETFYIARFLAAVAHSPLAAQLKPTTPFKRPPRLTRNSVLTNRIEEGER
  PDRAKGYELELSLALGTYYPPILLRQLLPTLLQGPSIFTAPKEVAEIKAQLETTLKSRNYEVKLRLLLHL
  EELQMEHDIRHYDLDSVPMTWDPVDQNPRLLTLEVPGVAESRPSVLRGDHLFALLSSETQQDDPVTYKGF
  VHKVELDRVKLSFSTSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWVLWPMLFPVASRGVSLLPS
  DVKFKLYDRSLESNPEQLQAMKHIVRGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPS
  NSGADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKTCCNWDAKKGEYVYPAKKHLQQYRVLITTLITA
  SRLVSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMDVKETGNPGGQLVLAGDPRQLGPVLRSPLALKH
  GLGYSLLERLLAYNSLYKKGPNGYDPQFITKLLRNYRSHPTILDIPNQLYYDGELQACADVVDRERFCRW
  EGLPQQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKQLLAPSSKKGKARLSPRNVGVISPYRKQ
  VEKIRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFN
  VAVTRAKALLIVVGNPLLLGHDPDWKTFLEFCKENGGYTGCPFPAKLDLQQGQDLLQGLSKLSPSTSGPR
  RHQNLPQEREGEGGLPLQVEPEWRNEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163440
• ORF Size: 3231 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163440.1, NP_001156912.1
• RefSeq Size: 3639 bp
• RefSeq ORF: 3234 bp
• Locus ID: 17454
• UniProt ID: P23249
• Cytogenetics: 3 F2.2
• Gene Summary: Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (By similarity). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7 (By similarity). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (PubMed:28662698). May function as a messenger ribonucleoprotein (mRNP) clearance factor (By similarity).[UniProtKB/Swiss-Prot Function]