Epx (NM_007946) Mouse Tagged ORF Clone

CAT#: MG215396

Epx (tGFP-tagged) - Mouse eosinophil peroxidase (Epx), (10ug)

Product Images

Circular map for MG215396

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Epx
• Synonyms: E; EPO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215396 representing NM_007946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGCAGCTTCTGGCCCTGGTAGGGGCCTTAGCCACACTCATCCTCACCCAACACGCTGAAGGCA
  CTGCCCCAGCTTCCCCCAGTCCAGTGGAGATTTCAGTCCTTCGAGACTGCATAGCAGAGGCTAAGCTGCT
  GGTGGACACTGCCTACAATCACACACAGAAAAGCATCATGCAACGTCTTCGCAGTGGCTCGGCCAGCCCC
  ATGGACCTCCTGGCCTACTTCAAACAGCCAGTAGCAGCCACCAGAAGAGTGGTTCAGGCCGCTGATTATA
  TGCATGTGGCCTTAGGGCTGCTAGAAGAGAGGCTGCAGCCACGGGGATCTAGACCCTTCAATGCCACTGA
  TGTGCTCACAGAACCACAGCTGCGCCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCAGGACCAGGCTGAG
  AGGTGCAGCAACAAGTACCGAACCATAACAGGGAGATGCAACAACAAGAAGCATCCCTGGCTGGGAGCCT
  CCAACCAGGCCCTGGCCCGCTGGCTGCCAGCTGAGTATGAAGACCACAGGTCTCTCCCCTTTGGCTGGAC
  CCCTGGAAAGAGGCGTAATGGCTTCCTGCTTCCTCTTGTCCGGGATGTCTCCAATCAGATTGTGCGCTTC
  CCCAGCAAGAAACTGACCTCAGACCGCGGCCGGGCCCTTATGTTCATGCAGTGGGGCCAGTTCATCGACC
  ATGACCTGGACTTCTCCCCGGAGTCCCCAGCCAGAGTGGCCTTCAGCATGGGTGTGGACTGTGAGAAGAC
  CTGTGCCCAGCTGCCCCCCTGCTTCCCCATCAAGATCCCACGCAATGATCCCCGCATCAAGAACCAGCGC
  GACTGCATCCCCTTCTTCCGCTCTGCCCCGGCGTGCCCCCAAAACAGGAACAAAGTCCGCAACCAGATCA
  ACGCACTTACATCCTTCGTGGACGCCAGCATGGTGTATGGCAGCGAGGTGACCCTCGCCCTGCGGCTCCG
  TAATAGGACCAACTTCCTGGGGCTGCTGGCCACGAACCAGCGATTCCAGGACAACGGTCGAGCCCTACTG
  CCTTTTGACAACCTGCATGAGGACCCCTGTCTCCTGACTAACCGCTCTGCACGCATCCCCTGCTTCCTGG
  CAGGTGACACTCGGTCAAGTGAAACCCCCAAACTGACGGCCTTGCACACGCTGTTTGTGAGAGAACACAA
  TCGCCTAGCCGCTGAGCTGAGGCGCCTGAATCCCCACTGGAGTGGAGACAAGCTGTACAACGAAGCCCGG
  AAGATCGTGGGAGCCATGGTCCAGATCATCACCTACCGAGACTTCCTGCCTCTGGTTCTGGGCAGGGCCC
  GGATCAGAAGAACTTTGGGTCCCTACAGAGGGTATTGCTCCAATGTGGACCCCCGTGTAGCCAATGTTTT
  CACCCTGGCCTTCCGCTTTGGCCACACCATGCTTCAGCCCTTCATGTTCCGCCTGGATAGCCAGTATCGG
  GCCTCGGCACCCAACTCCCACGTCCCGCTTAGCTCTGTATTCTTTGCCAGCTGGCGAATCATACATGAAG
  GTGGCATCGACCCTATCCTCCGAGGTCTCATGGCCACCCCAGCTAAGCTGAACCGGCAGGATTCCATGCT
  GGTGGATGAGCTCCGCGACAAGCTGTTTCAGCAAGTGAGAAGGATCGGGCTGGATCTGGCAGCTCTGAAC
  ATGCAGCGCAGCCGGGACCACGGCCTTCCAGGATACAATGCCTGGAGACGCTTCTGTGGGCTTTCCCAGC
  CCCGAAACTTGGCCCAGCTTAGTCGGGTGCTAAAAAATCAGGATTTGGCAAGGAAGTTTCTGAGACTGTA
  CAAGACGCCCGACAACATTGACATCTGGGTCGGAGCCATTGCGGAGCCTCTCCTGCCGGGGGCCCGAGTG
  GGGCCGCTTCTGGCTTGTCTTTTTGAGAACCAGTTCAGGAGAGCCAGAGATGGAGACAGATTCTGGTGGC
  AGAAATGGGGAGTTTTCACCAAAAGACAACGCAAAGCCCTGAGGCGAATCTCTTTATCTCGGATTGTATG
  CGACAATACTGGCATCACCACGGTTTCGAGGGACATCTTCAGGGCTAACATCTACCCACAGGGTTTTGTG
  AGCTGTAGCCGTATTCCCAAGTTGAACCTATCAGCCTGGCGAGGCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215396 representing NM_007946
  Red=Cloning site Green=Tags(s)
  
  MMQQLLALVGALATLILTQHAEGTAPASPSPVEISVLRDCIAEAKLLVDTAYNHTQKSIMQRLRSGSASP
  MDLLAYFKQPVAATRRVVQAADYMHVALGLLEERLQPRGSRPFNATDVLTEPQLRLLSQASGCALQDQAE
  RCSNKYRTITGRCNNKKHPWLGASNQALARWLPAEYEDHRSLPFGWTPGKRRNGFLLPLVRDVSNQIVRF
  PSKKLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFSMGVDCEKTCAQLPPCFPIKIPRNDPRIKNQR
  DCIPFFRSAPACPQNRNKVRNQINALTSFVDASMVYGSEVTLALRLRNRTNFLGLLATNQRFQDNGRALL
  PFDNLHEDPCLLTNRSARIPCFLAGDTRSSETPKLTALHTLFVREHNRLAAELRRLNPHWSGDKLYNEAR
  KIVGAMVQIITYRDFLPLVLGRARIRRTLGPYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYR
  ASAPNSHVPLSSVFFASWRIIHEGGIDPILRGLMATPAKLNRQDSMLVDELRDKLFQQVRRIGLDLAALN
  MQRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLRLYKTPDNIDIWVGAIAEPLLPGARV
  GPLLACLFENQFRRARDGDRFWWQKWGVFTKRQRKALRRISLSRIVCDNTGITTVSRDIFRANIYPQGFV
  SCSRIPKLNLSAWRGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007946
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007946.2, NP_031972.2
• RefSeq Size: 2691 bp
• RefSeq ORF: 2151 bp
• Locus ID: 13861
• UniProt ID: P49290
• Cytogenetics: 11 52.25 cM
• Gene Summary: This gene encodes a member of the peroxidase superfamily of enzymes that is produced by eosinophils and plays a critical role in eliminating tissue-invasive parasites. The encoded preproprotein undergoes proteolytic processing to generate a heterodimeric enzyme that forms a predominant component of the intracellular granules of eosinophils. Mice lacking the encoded protein exhibit resistance to ulcerative colitis induced by dextran sulfate. [provided by RefSeq, Jul 2016]