Lilrb3 (BC026937) Mouse Tagged ORF Clone

CAT#: MG210919

Lilrb3 (tGFP-tagged) - Mouse leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 (cDNA clone MGC:30225

Product Images

Circular map for MG210919

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lilrb3
• Synonyms: 6M1, 7M5, 16M4, 16M5, 16M6, PIRB1, PIRB2, PIRB3, PIRB4, PIRB5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210919 representing BC026937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGCACCTTCACAGCCCTGCTCTGTCTTGGACTGACTCTGAGCCTCTGGATCCCAGTGCTGACAG
  GGTCCCTCCCTAAGCCTATCCTCAGAGTACAGCCAGACTCTGTGGTCTCCAGGTGGACTAAGGTGACTTT
  CTTTTGTGAGGAGACAATTGGAGCCAATGAGTACCGCCTCTATAAAGATGGAAAGCTATATAAAACTGTA
  ACAAAGAACAAACAGAAGCCAGCAAACAAGGCTGAATTCTCACTCTCAAATGTAGACCTGAGTAATGCAG
  GTCAATATGAATGTTCCTACAGCACCCAGTATAAATCATCAGGCTACAGTGACCCCCTGAAGCTGGTGGT
  GACAGGACACTACTGGACACCCAGCCTTTTAGCCCAAGCCAGCCCTGTGGTAACTTCAGGAGGGTATGTC
  ACCCTCCAGTGTGAGTCCTGGCACAACGATCACAAGTTCATTCTGACTGTAGAAGGACCACAGAAGCTCT
  CGTGGACACAAGACTCACAGTATAATTACTCTACAAGGAAGTACCACGCCCTGTTCTCTGTGGGCCCTGT
  GACCCCCAACCAGAGATGGATATGCAGATGTTACAGTTATGACAGGAACAGACCATATGTGTGGTCACCT
  CCAAGTGAATCCGTGGAGCTCCTGGTCTCAGGTAATCTCCAAAAACCAACCATCAAGGCTGAACCAGGAT
  CTGTGATCACCTCCAAAAGAGCAATGACCATCTGGTGTCAGGGGAACCTGGATGCAGAAGTATATTTTCT
  GCATAATGAGGGAAGCCAAAAAACACAGAGCACACAGACCCTACAGCAGCCTGGGAACAAGGGCAAGTTC
  TTCATCCCTTCTATGACAAGACAACATGCAGGGCAATATCGCTGTTATTGTTACGGCTCAGCTGGTTGGT
  CACAGCCCAGTGACACCCTGGAGCTGGTGGTGACAGGAATCTATGAACACTATAAACCCAGGCTGTCAGT
  ACTGCCCAGCCCTGTGGTGACAGCAGGAGGAAACATGACACTCCACTGTGCCTCAGACTTTCACTACGAT
  AAATTCATTCTCACCAAGGAAGATAAGAAATTCGGCAACTCACTGGACACAGAGCATATATCTTCTAGTA
  GACAGTACCGAGCCCTGTTTATTATAGGACCCACAACCCCAACCCATACAGGGACATTCAGATGTTATGG
  TTACTTCAAGAATGCCCCACAGCTGTGGTCAGTACCTAGTGATCTCCAACAAATACTCATCTCAGGGCTG
  TCCAAGAAGCCCTCTCTGCTGACTCACCAAGGCCATATCCTGGACCCTGGAATGACCCTCACCCTGCAGT
  GTTACTCTGACATCAACTATGACAGATTTGCTCTGCACAAGGTGGGGGGAGCTGACATCATGCAGCACTC
  TAGCCAGCAGACTGACACTGGCTTCTCTGTGGCCAACTTCACACTGGGCTATGTGAGTAGCTCCACTGGA
  GGCCAATACAGATGCTATGGTGCACACAACCTTTCCTCTGAGTGGTCAGCCTCCAGTGAGCCCCTGGACA
  TCCTGATCACAGGACAGCTCCCTCTCACTCCTTCCCTCTCAGTGAAGCCTAACCACACAGTGCACTCAGG
  AGAGACCGTGAGCCTGCTGTGTTGGTCAATGGACTCTGTGGATACTTTCATTCTGTCCAAGGAGGGATCA
  GCCCAGCAACCCCTACGACTAAAATCAAAGTCCCATGATCAGCAGTCCCAGGCAGAATTCTCCATGAGTG
  CTGTGACCTCCCATCTCTCAGGCACCTACAGGTGCTATGGTGCTCAAAACTCATCTTTCTACCTCTTGTC
  ATCTGCCAGTGCCCCTGTGGAGCTCACAGTCTCAGGACCCATCGAAACCTCTACCCCGCCACCCACAATG
  TCCATGCCACTAGGTGGACTGCATATGTACCTGAAGGCTCTCATTGGAGTGTCTGTGGCCTTCATCCTGT
  TCCTCTTCATCCTCATCTTCATTCTTCTCCGACGAAGACATCGGGGAAAATTCAGGAAAGATGTCCAGAA
  AGAGAAAGACTTGCAACTTTCTTCAGGAGCTGAAGAGCCCATAACCAGGAAAGGAGAACTCCAGAAGAGG
  CCCAACCCAGCTGCTGCCACCCAGGAAGAAAGCCTATATGCTTCAGTGGAGGACATGCAAACTGAGGATG
  GAGTGGAGCTGAACAGCTGGACACCACCTGAGGAAGATCCCCAGGGAGAGACTTATGCCCAGGTGAAACC
  CTCCAGGCTCAGGAAGGCAGGACATGTCTCACCTTCTGTCATGTCAAGGGAACAACTGAACACAGAATAT
  GAACAAGCAGAAGAGGGCCAAGGAGCAAACAATCAGGCTGCCGAATCTGGGGAGTCCCAGGATGTGACCT
  ATGCCCAGCTGTGCAGCAGGACACTCAGACAGGGGGCAGCTGCATCTCCTCTCTCCCAGGCAGGGGAAGC
  CCCAGAGGAGCCCAGTGTATATGCTACCCTGGCGGCTGCTCGTCCAGAGGCTGTTCCCAAGGATGTGGAG
  CAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210919 representing BC026937
  Red=Cloning site Green=Tags(s)
  
  MSCTFTALLCLGLTLSLWIPVLTGSLPKPILRVQPDSVVSRWTKVTFFCEETIGANEYRLYKDGKLYKTV
  TKNKQKPANKAEFSLSNVDLSNAGQYECSYSTQYKSSGYSDPLKLVVTGHYWTPSLLAQASPVVTSGGYV
  TLQCESWHNDHKFILTVEGPQKLSWTQDSQYNYSTRKYHALFSVGPVTPNQRWICRCYSYDRNRPYVWSP
  PSESVELLVSGNLQKPTIKAEPGSVITSKRAMTIWCQGNLDAEVYFLHNEGSQKTQSTQTLQQPGNKGKF
  FIPSMTRQHAGQYRCYCYGSAGWSQPSDTLELVVTGIYEHYKPRLSVLPSPVVTAGGNMTLHCASDFHYD
  KFILTKEDKKFGNSLDTEHISSSRQYRALFIIGPTTPTHTGTFRCYGYFKNAPQLWSVPSDLQQILISGL
  SKKPSLLTHQGHILDPGMTLTLQCYSDINYDRFALHKVGGADIMQHSSQQTDTGFSVANFTLGYVSSSTG
  GQYRCYGAHNLSSEWSASSEPLDILITGQLPLTPSLSVKPNHTVHSGETVSLLCWSMDSVDTFILSKEGS
  AQQPLRLKSKSHDQQSQAEFSMSAVTSHLSGTYRCYGAQNSSFYLLSSASAPVELTVSGPIETSTPPPTM
  SMPLGGLHMYLKALIGVSVAFILFLFILIFILLRRRHRGKFRKDVQKEKDLQLSSGAEEPITRKGELQKR
  PNPAAATQEESLYASVEDMQTEDGVELNSWTPPEEDPQGETYAQVKPSRLRKAGHVSPSVMSREQLNTEY
  EQAEEGQGANNQAAESGESQDVTYAQLCSRTLRQGAAASPLSQAGEAPEEPSVYATLAAARPEAVPKDVE
  Q
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026937
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026937.1
• RefSeq Size: 2708 bp
• RefSeq ORF: 2525 bp
• Locus ID: 18733
• Cytogenetics: 7 2.14 cM
• Gene Summary: May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).[UniProtKB/Swiss-Prot Function]