Cd97 (BC006676) Mouse Tagged ORF Clone

CAT#: MG210300

Cd97 (tGFP-tagged) - Mouse CD97 antigen (cDNA clone MGC:5704 IMAGE:3499451)

Product Images

Circular map for MG210300

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd97
• Synonyms: AA409984; Cd97; TM7LN1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210300 representing BC006676
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGCGTCAGATGCCCCGGCCTGCTTGTGGTGTGCATTTTACTGAGTCTCTCAGGAGCTGGAACCC
  AGAAGGCAGAGAGTAAGAACTGTGCCAAGTGGTGCCCTATCAACTCAAAATGTGTCAGTAACAGAAGCTG
  TGTCTGCAAGCCCGGCTTCAGCTCGGAGAAGGAATTGATCACTAACCCTGCAGAGAGCTGTGAAGACATC
  AACGAGTGTTTACTACCTGGATTTTCCTGTGGAGACTTTGCCATGTGTAAGAACTCAGAGGGGAGTTACA
  CCTGCGTCTGTAACCTGGGATATAAGCTCCTTTCTGGTGCAGAATCTTTTGTTAACGAGAGTGAGAATAC
  ATGTCAAGATGTGGATGAGTGCAGCTCTGGGCAGCATCAATGTCACAATTCCACCGTCTGCAAAAACACC
  GTAGGCTCCTACAAGTGCCACTGCCGTCCAGGTTGGAAGCCAACTTCTGGGTCCCTCCGTGGTCCAGACA
  CCATATGCCAAGAGCCACCGTTCCCTACTTGGACACTGCTGCCCACAGCCCACAGTCAGACTCTCTTGAG
  ATTCTCTGTTGAAGTCCAGAATCTGCTCCGTGACTTCAATCCAGCCACGGTCAACTACACCATCCAGAAA
  CTCATCGAGGCTGTGGACAAGCTGCTGGAAGACCCCATGGAGACTGAAACCCAACAAGTAGCTGCCCAGT
  TGCTCTCGAACCTGGAACAAAGCCTTCGGACCTTGGCTCAGTTTTTACCCAAAGGCCCCTTCACCTACAC
  GTCCCCTTCAAATACTGAACTGTCCCTGATGGTGAAGGAGCAAGACAACAAAGATGTTACCACAGTGCAC
  CACGGCCAAACTTGGATGGAGCTGGACTGGGCTGTGACAGCTGGAGCCAAAATCTCAGAAAATGGCTCCT
  CGGTGGCAGGCATCCTGTCCAGCCCAAACATGGAAAAGCTGCTGGGCAACACCCCGCTGAACTTGGAGCA
  GAGAAGGGCCTCCTTGGAGGACTTCTATGGAAGCCCGATTCCGAGTGTCTCACTTAAGCTTCTCTCAAAC
  ATCAATTCTGTCTTCCTGACCAACACGAACACTGAAAAGCTCGCCTCGAATGTCACGTTCAAGTTCGACT
  TTACTTCTGTGGAGTCAATTGAGCCACGTCATGAGCTGATATGTGCCTTCTGGAAAGCCCACAACGGGAA
  TGGGTACTGGGACACCGACGGCTGCTCAATGAATGGCACTGGCTTCTGCCACTGCAACCACCTGACCAGC
  TTTGCCATCCTAATGGCTCAGTACCATGTGCAGGACCCAAGGCTGGAATTGATCACCAAGGTGGGGCTGT
  TGTTGTCCCTGATCTGCCTGCTGCTGTGCATCCTGACCTTCCTGCTGGTGAAGCCCATCCAGAGCTCTCG
  AACCATGGTGCACCTGCACCTGTGCATCTGCCTCTTCCTGGGCTCAATCATATTCCTGGTCGGCGTGGAG
  AATGAAGGGGGTGAGGTGGGCCTGCGCTGCCGCCTGGTGGCCGTGATGCTGCATTTCTGCTTCCTGGCCG
  CCTTCTGCTGGATGGCACTGGAGGGCGTGGAGCTCTACTTCCTGGTGGTGCGTGTGTTCCAGGGCCAAGG
  CCTGAGTACATGGCAACGCTGCCTGATTGGCTACGGGGTGCCCCTCCTCATCGTGGCCATCTCAATGGCA
  GTTGTCAAAATGGATGGCTATGGGCATGCAACATACTGCTGGCTGGACTTTAGGAAGCAGGGCTTTCTCT
  GGAGCTTTTCGGGACCCGTGGCCTTCATCATTTTCTGCAATGCTGCCATTTTCGTGATCACTGTCTGGAA
  GCTCACAAAGAAGTTTTCTGAAATCAACCCAAACATGAAGAAGTTAAGGAAGGCGAGGGTGCTGACCATC
  ACCTCCATCGCCCAGCTCCTTGTGCTGGGCTGCACCTGGGGCTTCGGCCTGTTCCTCTTCAACCCCCATA
  GCACATGGCTATCCTACATCTTTACCCTGCTCAACTGCCTGCAGGGCCTATTCCTCTATGTGATGCTCTG
  CCTGCTCAACAAAAAGGTGAGGGAAGAGTACTGGAAATGGGCCTGCATGGTCACTGGGAGCAAGTACACA
  GAGTTCAACTCTTCCACAACAGGCACTGGCACCAGCCAAACACGGGCTCTCAGGTCCTCAGAATCAGGGA
  TG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210300 representing BC006676
  Red=Cloning site Green=Tags(s)
  
  MRGVRCPGLLVVCILLSLSGAGTQKAESKNCAKWCPINSKCVSNRSCVCKPGFSSEKELITNPAESCEDI
  NECLLPGFSCGDFAMCKNSEGSYTCVCNLGYKLLSGAESFVNESENTCQDVDECSSGQHQCHNSTVCKNT
  VGSYKCHCRPGWKPTSGSLRGPDTICQEPPFPTWTLLPTAHSQTLLRFSVEVQNLLRDFNPATVNYTIQK
  LIEAVDKLLEDPMETETQQVAAQLLSNLEQSLRTLAQFLPKGPFTYTSPSNTELSLMVKEQDNKDVTTVH
  HGQTWMELDWAVTAGAKISENGSSVAGILSSPNMEKLLGNTPLNLEQRRASLEDFYGSPIPSVSLKLLSN
  INSVFLTNTNTEKLASNVTFKFDFTSVESIEPRHELICAFWKAHNGNGYWDTDGCSMNGTGFCHCNHLTS
  FAILMAQYHVQDPRLELITKVGLLLSLICLLLCILTFLLVKPIQSSRTMVHLHLCICLFLGSIIFLVGVE
  NEGGEVGLRCRLVAVMLHFCFLAAFCWMALEGVELYFLVVRVFQGQGLSTWQRCLIGYGVPLLIVAISMA
  VVKMDGYGHATYCWLDFRKQGFLWSFSGPVAFIIFCNAAIFVITVWKLTKKFSEINPNMKKLRKARVLTI
  TSIAQLLVLGCTWGFGLFLFNPHSTWLSYIFTLLNCLQGLFLYVMLCLLNKKVREEYWKWACMVTGSKYT
  EFNSSTTGTGTSQTRALRSSESGM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006676
• ORF Size: 2174 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006676, AAH06676
• RefSeq Size: 2737 bp
• RefSeq ORF: 2174 bp
• Locus ID: 26364
• Cytogenetics: 8 40.22 cM
• Gene Summary: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.[UniProtKB/Swiss-Prot Function]