Hlcs (NM_139145) Mouse Tagged ORF Clone

CAT#: MG210285

Hlcs (tGFP-tagged) - Mouse holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)

Product Images

Circular map for MG210285

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hlcs
• Synonyms: 410I21.SP6; D16Jhu34
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210285 representing NM_139145
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACAGGCTCCAGATGGACAACGGCCTGATAGCCCAGAAGATCGTTTCAGTCCACTTAAAGGACC
  CTGCTCTGAAGGAACTCGGCAAAGCCTCCGACAAGCAAGTGCAGGGCCCGCCCCCGGGCCCAGAAGCTTC
  CCCCGAGGCCCAGCCTGCACAAGGTGTCATGGAGCATGCTGGCCAAGGTGATTGCAAGGCCGCCGGTGAA
  GGCCCTTCCCCACGGAGGAGGGGCTGTGCCCCTGAAAGTGAGCCTGCTGCAGATGGGGACCCAGGGCTGA
  GCTCCCCGGAGCTCTGTCAGCTCCACCTGTCCATCTGTCACGAGTGTCTGGAGCTCGAGAACAGTACCAT
  TGACTCGGTGAGGTCTGCGTCTGCGGAGAACATTCCAGACCTTCCCTGTGATCACAGCGGCGTGGAGGGT
  GCTGCGGGCGAACTCTGCCCCGAACGGAAAGGGAAGAGAGTCAACATTTCGGGAAAGGCGCCCAATATCT
  TGCTGTATGTGGGCTCTGGTTCCGAGGAAGCCCTGGGCCGGCTCCAGCAGGTGCGATCTGTCCTGACTGA
  CTGTGTGGACACGGACAGCTACACTCTCTACCACCTGCTGGAAGACAGCGCTCTGAGGGACCCGTGGTCT
  GACAACTGCCTCCTGCTAGTTATTGCCAGCAGGGACCCCATCCCCAAAGACATACAGCACAAGTTCATGG
  CCTATCTTTCTCAGGGAGGGAAGGTGCTGGGCCTGTCTTCCCCCTTCACGTTGGGTGGCTTTCGGGTGAC
  CAGGAGAGATGTGCTACGGAACACAGTCCAGAACTTGGTTTTCTCTAAGGCTGATGGGACTGAGGTACGG
  CTCAGTGTCCTGAGCAGCGGCTACGTTTATGAAGAAGGCCCGAGCTTGGGCAGGCTCCAGGGCCACCTGG
  AGAATGAGGACAAGGATAAAATGATTGTGCACGTGCCTTTTGGAACCCTCGGAGGGGAAGCCGTTCTCTG
  CCAGGTGCACCTGGAGCTCCCGCCCGGCGCCTCCCTGGTGCAGACTGCAGACGACTTTAACGTGCTCAAA
  TCCAGCAATGTGAGAAGACACGAGGTCCTTAAGGAGATCCTGACTGCCCTCGGTCTGAGCTGTGACGCAC
  CACAGGTTCCTGCCTTAACGCCCCTGTACTTACTGCTGGCCGCTGAGGAAACCCAGGATCCGTTCATGCA
  GTGGCTCGGGAGGCACACAGATCCTGAAGGAATCATAAAATCCAGCAAGCTCTCCCTTCAGTTTGTTTCC
  TCCTACACATCTGAAGCGGAGATCACCCCGTCTTCCATGCCTGTGGTCACTGACCCAGAAGCCTTTTCAT
  CAGAGCACTTCAGCCTAGAGACCTATCGACAGAATCTGCAGACCACGCGCCTTGGGAAAGTGATTCTGTT
  TGCAGAAGTGACCTCCACCACCATGAGTCTCCTGGATGGGTTGATGTTTGAGATGCCGCAGGAAATGGGC
  TTAATTGCCATTGCAGTTCGGCAGACCCAGGGCAAAGGAAGGGGCCCGAATGCCTGGCTGAGCCCCGTGG
  GATGTGCTCTTTCCACTCTGCTGGTCTTCATCCCCCTGAGGTCACAACTGGGACAGAGGATTCCATTTGT
  CCAGCATTTGATGTCCTTGGCTGTGGTGGAGGCAGTGCGGTCTATCCCAGGGTATGAGGATATCAACTTG
  CGAGTGAAGTGGCCCAACGATATTTATTACAGCGATCTCATGAAGATCGGTGGGGTTCTGGTTAATTCAA
  CACTTATGGGAGAAACATTTTATATCCTCATCGGCTGTGGATTCAATGTGACGAACAGTAATCCTACCAT
  TTGTATCAACGACCTCATCGAAGAACACAATAAGCAGCACGGGGCGGGCCTGAAGCCCCTGCGAGCGGAC
  TGTCTCATAGCCAGAGCCGTGACCGTGCTGGAGAAACTGATCGACAGGTTTCAGGACCAAGGGCCCGACG
  GCGTTCTTCCCCTCTATTATAAATACTGGGTGCACGGTGGTCAGCAAGTCCGCCTGGGCAGCACGGAGGG
  GCCACAGGCATCTATTGTGGGCCTTGATGACTCCGGGTTCCTGCAGGTCCACCAAGAGGATGGTGGGGTT
  GTGACGGTGCATCCAGATGGCAACTCCTTCGACATGCTGAGAAACCTCATCGTGCCCAAGCGACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210285 representing NM_139145
  Red=Cloning site Green=Tags(s)
  
  MEDRLQMDNGLIAQKIVSVHLKDPALKELGKASDKQVQGPPPGPEASPEAQPAQGVMEHAGQGDCKAAGE
  GPSPRRRGCAPESEPAADGDPGLSSPELCQLHLSICHECLELENSTIDSVRSASAENIPDLPCDHSGVEG
  AAGELCPERKGKRVNISGKAPNILLYVGSGSEEALGRLQQVRSVLTDCVDTDSYTLYHLLEDSALRDPWS
  DNCLLLVIASRDPIPKDIQHKFMAYLSQGGKVLGLSSPFTLGGFRVTRRDVLRNTVQNLVFSKADGTEVR
  LSVLSSGYVYEEGPSLGRLQGHLENEDKDKMIVHVPFGTLGGEAVLCQVHLELPPGASLVQTADDFNVLK
  SSNVRRHEVLKEILTALGLSCDAPQVPALTPLYLLLAAEETQDPFMQWLGRHTDPEGIIKSSKLSLQFVS
  SYTSEAEITPSSMPVVTDPEAFSSEHFSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMG
  LIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYEDINL
  RVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLIEEHNKQHGAGLKPLRAD
  CLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGGQQVRLGSTEGPQASIVGLDDSGFLQVHQEDGGV
  VTVHPDGNSFDMLRNLIVPKRQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139145
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139145.5
• RefSeq Size: 4833 bp
• RefSeq ORF: 2169 bp
• Locus ID: 110948
• UniProt ID: Q920N2
• Cytogenetics: 16 55.12 cM
• Gene Summary: Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase (By similarity).[UniProtKB/Swiss-Prot Function]