Hlcs (NM_139145) Mouse Untagged Clone

CAT#: MC204751

Hlcs (untagged) - Mouse holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) (Hlcs), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hlcs
• Synonyms: 410I21.SP6; D16Jhu34
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC050090
  ACATTTGTGACAGAGAGCATTTGGATACAAGGTACCAGTTTACAAGAGCCATCATCTTCAGAAACCGTTG TAAAATGGTCTGACTGCTGTCTGCCGTTAGCCTGCCGACCCGGGGACCCCTACCAGTTAATTGCTAAGGC AAGCGTGGACAACTTCAGCAAGCTTGGGGTGGCCTTCATGGAGGACAGGCTCCAGATGGACAACGGCCTG ATAGCCCAGAAGATCGTTTCAGTCCACTTAAAGGACCCTGCTCTGAAGGAACTCGGCAAAGCCTCCGACA AGCAAGTGCAGGGCCCGCCCCCGGGCCCAGAAGCTTCCCCCGAGGCCCAGCCTGCACAAGGTGTCATGGA GCATGCTGGCCAAGGTGATTGCAAGGCCGCCGGTGAAGGCCCTTCCCCACGGAGGAGGGGCTGTGCCCCT GAAAGTGAGCCTGCTGCAGATGGGGACCCAGGGCTGAGCTCCCCGGAGCTCTGTCAGCTCCACCTGTCCA TCTGTCACGAGTGTCTGGAGCTCGAGAACAGTACCATTGACTCGGTGAGGTCTGCGTCTGCGGAGAACAT TCCAGACCTTCCCTGTGATCACAGCGGCGTGGAGGGTGCTGCGGGCGAACTCTGCCCCGAACGGAAAGGG AAGAGAGTCAACATTTCGGGAAAGGCGCCCAATATCTTGCTGTATGTGGGCTCTGGTTCCGAGGAAGCCC TGGGCCGGCTCCAGCAGGTGCGATCTGTCCTGACTGACTGTGTGGACACGGACAGCTACACTCTCTACCA CCTGCTGGAAGACAGCGCTCTGAGGGACCCGTGGTCTGACAACTGCCTCCTGCTAGTTATTGCCAGCAGG GACCCCATCCCCAAAGACATACAGCACAAGTTCATGGCCTATCTTTCTCAGGGAGGGAAGGTGCTGGGCC TGTCTTCCCCCTTCACGTTGGGTGGCTTTCGGGTGACCAGGAGAGATGTGCTACGGAACACAGTCCAGAA CTTGGTTTTCTCTAAGGCTGATGGGACTGAGGTACGGCTCAGTGTCCTGAGCAGCGGCTACGTTTATGAA GAAGGCCCGAGCTTGGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGATAAAATGATTGTGCACG TGCCTTTTGGAACCCTCGGAGGGGAAGCCGTTCTCTGCCAGGTGCACCTGGAGCTCCCGCCCGGCGCCTC CCTGGTGCAGACTGCAGACGACTTTAACGTGCTCAAATCCAGCAATGTGAGAAGACACGAGGTCCTTAAG GAGATCCTGACTGCCCTCGGTCTGAGCTGTGACGCACCACAGGTTCCTGCCTTAACGCCCCTGTACTTAC TGCTGGCCGCTGAGGAAACCCAGGATCCGTTCATGCAGTGGCTCGGGAGGCACACAGATCCTGAAGGAAT CATAAAATCCAGCAAGCTCTCCCTTCAGTTTGTTTCCTCCTACACATCTGAAGCGGAGATCACCCCGTCT TCCATGCCTGTGGTCACTGACCCAGAAGCCTTTTCATCAGAGCACTTCAGCCTAGAGACCTATCGACAGA ATCTGCAGACCACGCGCCTTGGGAAAGTGATTCTGTTTGCAGAAGTGACCTCCACCACCATGAGTCTCCT GGATGGGTTGATGTTTGAGATGCCGCAGGAAATGGGCTTAATTGCCATTGCAGTTCGGCAGACCCAGGGC AAAGGAAGGGGCCCGAATGCCTGGCTGAGCCCCGTGGGATGTGCTCTTTCCACTCTGCTGGTCTTCATCC CCCTGAGGTCACAACTGGGACAGAGGATTCCATTTGTCCAGCATTTGATGTCCTTGGCTGTGGTGGAGGC AGTGCGGTCTATCCCAGGGTATGAGGATATCAACTTGCGAGTGAAGTGGCCCAACGATATTTATTACAGC GATCTCATGAAGATCGGTGGGGTTCTGGTTAATTCAACACTTATGGGAGAAACATTTTATATCCTCATCG GCTGTGGATTCAATGTGACGAACAGTAATCCTACCATTTGTATCAACGACCTCATCGAAGAACACAATAA GCAGCACGGGGCGGGCCTGAAGCCCCTGCGAGCGGACTGTCTCATAGCCAGAGCCGTGACCGTGCTGGAG AAACTGATCGACAGGTTTCAGGACCAAGGGCCCGACGGCGTTCTTCCCCTCTATTATAAATACTGGGTGC ACGGTGGTCAGCAAGTCCGCCTGGGCAGCACGGAGGGGCCACAGGCATCTATTGTGGGCCTTGATGACTC CGGGTTCCTGCAGGTCCACCAAGAGGATGGTGGGGTTGTGACGGTGCATCCAGATGGCAACTCCTTCGAC ATGCTGAGAAACCTCATCGTGCCCAAGCGACAGTAACACCTTCCTGTGTCCCCAGACAGCTTGGATCTAC TGCAACAGGGAAGCTGCTCCTGGGATCTCCTGGGAGCACTGAGCAGGCGCACTCCGCTTGCATAACTGTT GTCTCTGCCTGCCTAGGGCCTGGGAAGCTAATGCAGAGTTAGAGGCCAGTTGTTAATTCTTCAGGTTGTG GGTTCCCTGCCCCCACCCCCTCCCCACCCCGGGATTTGGGACACATATATGTGGTTTTACTGGGTGTTTG AAGGTGCATCTGGTGAGGATTGACTCACAGGCGAAGAAGGGTCAGTTTAGCCTAGGAGTTTAGTTTGCCT TGTGAGGTATAGAAGTGCTCTTCCCCATGTGGGCTTGGATTTGTGTGTACTGGGAGGGAAATGAGAAGCA AGGGGTTAGGAATAGCGCTGAGATAGTACAAATATATCCCAAGGAGTGAAGGCCTTTAGCCCTCTGCTGT TGAAACAGTGAATGCCTACAGCTCAGGGGCATGCTCACTGCTGTCCCAGAACACACAAGTGTTGACCTGC ACGTAGGGTTGTTGCTGGTTTCCTTTGTCAAGTTGGGCTTCTTGTAGTAGCCTGGAACACAGGACTGAAG GATGGCCACAGAAATGCTGCTAACCAGCGTTATCTCCTTTCCAGGATCAGATGTGTCAGGTTTTAGATGT TTAAGCCATGATGGCAGCTTCCTTAGAGACAAAGGCTTGCCCAGGCTGATGGAGACGTGGGCTCACCCTC TTGCCCACACAAGGGAGGATTCTTGGAGCTTTTAAGCTGATCAGTTGCAGGCTCTGTTAGAATGCCACAT CCCCATAAGCTTGTGTCCCCAAGCAGGGTCGAGTGCAGCACTGTATTGTAAGTCATGACTTTCTGCCCCT AATTTCATAATGTCAGGAAGGAGAGAGAGAGAGACTGACACAAAAATGCCATCAGTTTGGAGAGACGTAT GTGCGTGCAGAGCATGCCTGGTTTTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTTTAAA AGAAGATGTTCAAAACTTGATGTGCTAAAACGTCTCCGTGGAAGAACAAAGGAGAGGAAGCCTTTCTGGG CTTGCGTTTCCAGATGTGCTCCGTGCTTTCCCGTGTACCTCCCGTCTCTGTGGAGCTTGTGAATCATACC CATCGCTGCCCAGCCTGAAAGAACTAGTTCCTGTCTGCCCTCCTGCTTCCTGCCACTTCCACTGAAGTGG GCGGACGGAACAGATCGCTTCGCTTAGAGCAAGATCCTCGTCATAAACGAGTACCATCTTTATCCAGCTT CTGTAGTCAGGGCCAACTCCAGAAACCAGAGGTCTTCGGCATAGAATCCACTCACCCTCAGCCTAGCAGA GTCAGCCCCATTTGAAGCAAAAAATAAAATAAATAAATAACAGGGTGAACTATACCAACCTGACGTGTGT TTGAGGGGCTGTGACACTAAAGGAACTTGAAAGTAACTTTTCTTGCAAATAAGACTTTGACTAATGTTTT TCTCAAAAAAAGAAAAAGATACATATATAAAATTTAGTTCCGCTATTTTTTTTAAATTCATGCGAGTTTA AATAAAACTGTACGCCACCCCAAATTATAAAGTGACAGGCCCAAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGAGAGAGAGCGAGAGCATCTGTAAAATATCATCAAGAATGAAGATCTCCAAGCTGGCTGTTG CAAAGGTTCATTTCTCAGACTTTGTAATATAAGGCTGTTCATCTCTCAGAACCGCCATCATTACATTTGA AATTTCTATGGGGGATTATTTAATGAAAAACATGCTATGTTTTATTTTAAGCTGAAGGCCTATTCTGGAT AGTTCTACTTTGGGGAAAAAAATGTTATCATTTAATTTCCTTTCTGTAAGTTAAAACTAATGAAGTGTGG TCATGTCAGAGCAGACAGAGATGTTCTGGGCATGCTGATTGGCCCTGGGGTAGTTACACTGTTTGGGGGT ATTCTTGCGTATGCAGACAGGACACTCCTTGTCTCGAGAGACAGCACAGCCTGAAGCACTGAAGCTGCAT TCCCCACTGGCCTGACTTGGCCTCACATGAACCAAAGACTTCAGATTTCACATCGTAGAGAGCATTTCTG CCGATGGGGCGTGTAAAGGCTGCCACTGTACCCGCCACATGCGAGGGTATTCACACTCTCAGTGTCCCAG GACCTCCAAGAACTTTACAAGTCTCCTAACCTCGATGCCTCCTAATCTGGAGACGTGGCCACCTAACCCC GACTCTGTATTTGCCGTAAGCAGGTTACTCCTAGTGTTAACCTGCTTGCATATCTACCCCTGGGTGTTGG AAGGCACAGGTAAGCAGGCACTGGGTCTCACACAACTCAGCTGAAGCAGTGAGCGGCTTTGCTACCCTGA CATGCCAGCAACGGGGCAACCTCATTCCTGGTTCATCTCTATGCATCTTTGAAATAAACAAAAATGTAAA CCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_139145
• Insert Size: 4833 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050090, AAH50090
• RefSeq Size: 4880 bp
• RefSeq ORF: 4833 bp
• Locus ID: 110948
• UniProt ID: Q920N2
• Cytogenetics: 16 55.12 cM
• Gene Summary: Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.