Flt1 (BC029674) Mouse Tagged ORF Clone

CAT#: MG210050

Flt1 (tGFP-tagged) - Mouse FMS-like tyrosine kinase 1 (cDNA clone MGC:36074 IMAGE:5368921)

Product Images

Circular map for MG210050

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Flt1
• Synonyms: VEGFR-1, VEGFR1, sFlt1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210050 representing BC029674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTGCTGGGACACCGCGGTCTTGCCTTACGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATG
  GCTCAGGGTCGAAGTTAAAAGTGCCTGAACTGAGTTTAAAAGGCACCCAGCATGTCATGCAAGCAGGCCA
  GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCATGGTCTCTGCCCACGACCGTGAGCCAGGAG
  GACAAAAGGCTGAGCATCACTCCCCCATCGGCCTGTGGGAGGGATAACAGGCAATTCTGCAGCACCTTGA
  CCTTGGACACGGCGCAGGCCAACCACACGGGCCTCTACACCTGTAGATACCTCCCTACATCTACTTCGAA
  GAAAAAGAAAGCGGAATCTTCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCAC
  ACTGACATACCCAAACTTGTGCACATGACGGAAGGAAGACAGCTCATCATCCCCTGCCGGGTGACGTCAC
  CCAACGTCACAGTCACCCTAAAAAAGTTTCCATTTGATACTCTTACCCCTGATGGGCAAAGAATAACATG
  GGACAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGAACTGCGAAGCC
  ACCGTCAACGGGCACCTGTACCAGACAAACTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCC
  AAATACGCCCGCCGAGCCCAGTGAGACTGCTCCACGGGCAGACTCTTGTCCTCAACTGCACCGCCACCAC
  GGAGCTCAATACGAGGGTGCAAATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATAAGG
  CAGCGGATTGACCGGTGCCATTCCCACAACAATGTGTTCCACAGTGTTCTTAAGATCAACAATGTGGAGA
  GCCGAGACAAGGGGCTCTACACCTGTCGCGTGAAGAGTGGGTCCTCGTTCCAGTCTTTCAACACCTCCGT
  GCATGTGTATGAAAAAGGATTCATCAGTGTGAAACATCGGAAGCAGCCGGTGCAGGAAACCACAGCAGGA
  AGACGGTCCTATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCCCCAGAAATCGTATGGTTAAAAGATG
  GCTCGCCTGCAACATTGAAGTCTGCTCGCTATTTGGTACATGGCTACTCATTAATTATCAAAGATGTGAC
  AACCGAGGATGCAGGGGACTATACGATCTTGCTGGGCATAAAGCAGTCAAGGCTATTTAAAAACCTCACT
  GCCACTCTCATTGTAAACGTGAAACCTCAGATCTACGAAAAGTCCGTGTCCTCGCTTCCAAGCCCACCTC
  TCTATCCGCTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCGGCCAACAATCACGTG
  GCTCTGGCACCCCTGTCACCACAATCACTCCAAAGAAAGGTATGACTTCTGCACTGAGAATGAAGAATCC
  TTTATCCTGGATCCCAGCAGCAACTTAGGAAACAGAATTGAGAGCATCTCTCAGCGCATGACGGTCATAG
  AAGGAACAAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCAGACCCCTGGAATCTACAGCTGCCG
  GGCCTTCAATAAAATAGGGACTGTGGAAAGAAACATAAAATTTTATGTCACAGATGTGCCGAATGGCTTT
  CACGTTTCCTTGGAAAAGATGCCAGCCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAATAAATTCC
  TGTACAGAGACATTACCTGGATTCTGCTACGGACAGTTAACAACAGAACCATGCACCATAGTATCAGCAA
  GCAAAAAATGGCCACCACTCAAGATTACTCCATCACTCTGAACCTTGTCATCAAGAACGTGTCTCTAGAA
  GACTCGGGCACCTATGCGTGCAGAGCCAGGAACATATACACAGGGGAAGACATCCTTCGGAAGACAGAAG
  TTCTCGTTAGAGGTGAGCACTGCGGCAAAAAGGCCATTTTCTCTCGGATCTCCAAATTTAAAAGCAGGAG
  GAATGATTGTACCACACAAAGTCATGTCAAACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210050 representing BC029674
  Red=Cloning site Green=Tags(s)
  
  MVSCWDTAVLPYALLGCLLLTGYGSGSKLKVPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQE
  DKRLSITPPSACGRDNRQFCSTLTLDTAQANHTGLYTCRYLPTSTSKKKKAESSIYIFVSDAGSPFIEMH
  TDIPKLVHMTEGRQLIIPCRVTSPNVTVTLKKFPFDTLTPDGQRITWDSRRGFIIANATYKEIGLLNCEA
  TVNGHLYQTNYLTHRQTNTILDVQIRPPSPVRLLHGQTLVLNCTATTELNTRVQMSWNYPGKATKRASIR
  QRIDRCHSHNNVFHSVLKINNVESRDKGLYTCRVKSGSSFQSFNTSVHVYEKGFISVKHRKQPVQETTAG
  RRSYRLSMKVKAFPSPEIVWLKDGSPATLKSARYLVHGYSLIIKDVTTEDAGDYTILLGIKQSRLFKNLT
  ATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPRPTITWLWHPCHHNHSKERYDFCTENEES
  FILDPSSNLGNRIESISQRMTVIEGTNKTVSTLVVADSQTPGIYSCRAFNKIGTVERNIKFYVTDVPNGF
  HVSLEKMPAEGEDLKLSCVVNKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLE
  DSGTYACRARNIYTGEDILRKTEVLVRGEHCGKKAIFSRISKFKSRRNDCTTQSHVKH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC029674
• ORF Size: 2064 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029674, AAH29674
• RefSeq Size: 3218 bp
• RefSeq ORF: 2066 bp
• Locus ID: 14254
• Cytogenetics: 5 87.01 cM
• Gene Summary: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).[UniProtKB/Swiss-Prot Function]