Flt1 (BC029674) Mouse Untagged Clone

CAT#: MC220689

Flt1 (untagged) - Mouse FMS-like tyrosine kinase 1 (cDNA clone MGC:36074 IMAGE:5368921), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Flt1
• Synonyms: VEGFR-1, VEGFR1, sFlt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC029674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGCTGCTGGGACACCGCGGTCTTGCCTTACGCGCTGCTCGGGTGTCTGCTTCTCACAGGATATG
  GCTCAGGGTCGAAGTTAAAAGTGCCTGAACTGAGTTTAAAAGGCACCCAGCATGTCATGCAAGCAGGCCA
  GACTCTCTTTCTCAAGTGCAGAGGGGAGGCAGCCCACTCATGGTCTCTGCCCACGACCGTGAGCCAGGAG
  GACAAAAGGCTGAGCATCACTCCCCCATCGGCCTGTGGGAGGGATAACAGGCAATTCTGCAGCACCTTGA
  CCTTGGACACGGCGCAGGCCAACCACACGGGCCTCTACACCTGTAGATACCTCCCTACATCTACTTCGAA
  GAAAAAGAAAGCGGAATCTTCAATCTACATATTTGTTAGTGATGCAGGGAGTCCTTTCATAGAGATGCAC
  ACTGACATACCCAAACTTGTGCACATGACGGAAGGAAGACAGCTCATCATCCCCTGCCGGGTGACGTCAC
  CCAACGTCACAGTCACCCTAAAAAAGTTTCCATTTGATACTCTTACCCCTGATGGGCAAAGAATAACATG
  GGACAGTAGGAGAGGCTTTATAATAGCAAATGCAACGTACAAAGAGATAGGACTGCTGAACTGCGAAGCC
  ACCGTCAACGGGCACCTGTACCAGACAAACTATCTGACCCATCGGCAGACCAATACAATCCTAGATGTCC
  AAATACGCCCGCCGAGCCCAGTGAGACTGCTCCACGGGCAGACTCTTGTCCTCAACTGCACCGCCACCAC
  GGAGCTCAATACGAGGGTGCAAATGAGCTGGAATTACCCTGGTAAAGCAACTAAGAGAGCATCTATAAGG
  CAGCGGATTGACCGGTGCCATTCCCACAACAATGTGTTCCACAGTGTTCTTAAGATCAACAATGTGGAGA
  GCCGAGACAAGGGGCTCTACACCTGTCGCGTGAAGAGTGGGTCCTCGTTCCAGTCTTTCAACACCTCCGT
  GCATGTGTATGAAAAAGGATTCATCAGTGTGAAACATCGGAAGCAGCCGGTGCAGGAAACCACAGCAGGA
  AGACGGTCCTATCGGCTGTCCATGAAAGTGAAGGCCTTCCCCTCCCCAGAAATCGTATGGTTAAAAGATG
  GCTCGCCTGCAACATTGAAGTCTGCTCGCTATTTGGTACATGGCTACTCATTAATTATCAAAGATGTGAC
  AACCGAGGATGCAGGGGACTATACGATCTTGCTGGGCATAAAGCAGTCAAGGCTATTTAAAAACCTCACT
  GCCACTCTCATTGTAAACGTGAAACCTCAGATCTACGAAAAGTCCGTGTCCTCGCTTCCAAGCCCACCTC
  TCTATCCGCTGGGCAGCAGACAAGTCCTCACTTGCACCGTGTATGGCATCCCTCGGCCAACAATCACGTG
  GCTCTGGCACCCCTGTCACCACAATCACTCCAAAGAAAGGTATGACTTCTGCACTGAGAATGAAGAATCC
  TTTATCCTGGATCCCAGCAGCAACTTAGGAAACAGAATTGAGAGCATCTCTCAGCGCATGACGGTCATAG
  AAGGAACAAATAAGACGGTTAGCACATTGGTGGTGGCTGACTCTCAGACCCCTGGAATCTACAGCTGCCG
  GGCCTTCAATAAAATAGGGACTGTGGAAAGAAACATAAAATTTTATGTCACAGATGTGCCGAATGGCTTT
  CACGTTTCCTTGGAAAAGATGCCAGCCGAAGGAGAGGACCTGAAACTGTCCTGTGTGGTCAATAAATTCC
  TGTACAGAGACATTACCTGGATTCTGCTACGGACAGTTAACAACAGAACCATGCACCATAGTATCAGCAA
  GCAAAAAATGGCCACCACTCAAGATTACTCCATCACTCTGAACCTTGTCATCAAGAACGTGTCTCTAGAA
  GACTCGGGCACCTATGCGTGCAGAGCCAGGAACATATACACAGGGGAAGACATCCTTCGGAAGACAGAAG
  TTCTCGTTAGAGGTGAGCACTGCGGCAAAAAGGCCATTTTCTCTCGGATCTCCAAATTTAAAAGCAGGAG
  GAATGATTGTACCACACAAAGTCATGTCAAACATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC029674
• Insert Size: 2067 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029674, AAH29674
• RefSeq Size: 3218 bp
• RefSeq ORF: 2066 bp
• Locus ID: 14254
• Cytogenetics: 5 87.01 cM
• Gene Summary: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).[UniProtKB/Swiss-Prot Function]