Shmt1 (NM_009171) Mouse Tagged ORF Clone

CAT#: MG207663

Shmt1 (tGFP-tagged) - Mouse serine hydroxymethyltransferase 1 (soluble) (Shmt1)

Product Images

Circular map for MG207663

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Shmt1
• Synonyms: AI324848; AI385541; C81125; mshmt; mshmt1; mshmt2; Shmt
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207663 representing NM_009171
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACAGGGATGCCACCCTGTGGGCTTCTCATGAGAAGATGTTGTCGCAGCCCCTCAAGGACAGTG
  ATGCCGAGGTTTACAGCATCATTAAAAAGGAGAGCAACCGGCAGAGGGTCGGATTAGAGCTGATTGCCTC
  GGAGAATTTTGCCAGCCGAGCTGTGTTGGAGGCCCTGGGATCTTGCTTAAATAACAAATACTCTGAGGGA
  TATCCAGGCCAAAGGTATTATGGCGGGACCGAGTTCATCGATGAGCTGGAGATGCTGTGTCAGAAGCGTG
  CATTACAGGCCTACCATCTGGATCCTCAGTGCTGGGGTGTCAATGTCCAGCCTTATTCAGGCTCCCCTGC
  AAACTTTGCTGTGTATACTGCCCTGGTAGAGCCTCATGGGCGAATCATGGGCTTGGACCTGCCAGACGGT
  GGCCATCTGACCCATGGCTTCATGACAGACAAGAAGAAAATCTCTGCCACATCTATCTTCTTTGAATCCA
  TGCCTTATAAGGTGTACCCAGAGACTGGGTACATCAACTATGACCAGCTGGAGGAGAATGCCAGCCTCTT
  CCACCCGAAGCTGATCATCGCAGGGACCAGCTGCTACTCCCGGAACCTGGACTACGCACGGCTGCGGAAG
  ATTGCGGATGATAATGGGGCGTATCTCATGGCAGACATGGCACATATCAGTGGGCTCGTGGCAGCTGGGG
  TGGTGCCCTCCCCCTTTGAACACTGCCATGTAGTGACCACTACAACCCACAAGACCCTGAGAGGCTGCCG
  TGCTGGTATGATATTCTACAGAAAGGGTGTGCGCAGTGTGGATCCCAAGACTGGCAAAGAGACTTACTAT
  GAACTGGAGTCCCTCATCAACTCTGCCGTGTTCCCGGGCCTTCAGGGGGGTCCTCACAACCATGCCATTG
  CTGGTGTTGCTGTGGCCCTGAAGCAAGCCATGACTACAGAATTCAAAATCTACCAGCTCCAGGTGTTGGC
  CAACTGCAGGGCTCTGTCTGATGCACTCACAGAGCTAGGCTACAAAATAGTCACAGGTGGCTCTGATAAT
  CACTTGATCCTAATGGATCTGCGTTCTAAGGGTACTGATGGTGGAAGGGCTGAGAAGGTACTAGAAGCCT
  GTTCTATCGCCTGCAACAAGAATACCTGCCCAGGTGACAAGAGTGCGTTACGGCCCAGTGGACTGCGACT
  GGGGACTCCAGCATTGACATCCCGAGGACTTTTGGAAGAAGACTTCCAAAAAGTAGCACACTTTATCCAC
  AGGGGGATAGAGTTGACACTGCAGATTCAGAGCCACATGGCCACGAAGGCCACACTGAAAGAGTTCAAAG
  AGAAGCTGGCAGGAGATGAGAAGATCCAGAGTGCTGTGGCAACTCTCCGGGAAGAAGTGGAGAACTTTGC
  TTCCAACTTCTCACTACCTGGCCTTCCTGACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207663 representing NM_009171
  Red=Cloning site Green=Tags(s)
  
  MADRDATLWASHEKMLSQPLKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEG
  YPGQRYYGGTEFIDELEMLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDG
  GHLTHGFMTDKKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRK
  IADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVRSVDPKTGKETYY
  ELESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDN
  HLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIH
  RGIELTLQIQSHMATKATLKEFKEKLAGDEKIQSAVATLREEVENFASNFSLPGLPDF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009171
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009171.2, NP_033197.2
• RefSeq Size: 2074 bp
• RefSeq ORF: 1437 bp
• Locus ID: 20425
• UniProt ID: P50431
• Cytogenetics: 11 B2
• Gene Summary: Interconversion of serine and glycine.[UniProtKB/Swiss-Prot Function]