Wnt2 (NM_023653) Mouse Tagged ORF Clone

CAT#: MG205513

Wnt2 (tGFP-tagged) - Mouse wingless-related MMTV integration site 2 (Wnt2)

Product Images

Circular map for MG205513

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt2
• Synonyms: 2610510E18Rik; Int1l1; Irp; Mirp; Wnt-2; Wnt2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205513 representing NM_023653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGTCCCTCTCGGTGGAATCTGGCTCTGGCTCCCTCTGCTCTTGACCTGGCTCACCCCTGAGGTCA
  GCTCTTCATGGTGGTACATGAGAGCTACAGGTGGCTCCTCCAGGGTGATGTGTGACAATGTGCCAGGCCT
  GGTGAGCCGGCAGCGTCAGCTGTGCCACCGACACCCAGATGTGATGCGTGCCATTGGCCTGGGTGTGGCT
  GAGTGGACTGCAGAGTGCCAACACCAGTTCCGCCAGCATCGCTGGAACTGCAACACCCTGGACAGAGATC
  ACAGCCTCTTTGGCCGGGTCCTCCTCCGAAGTAGTCGAGAATCGGCCTTTGTTTACGCCATCTCTTCAGC
  TGGCGTTGTATTTGCCATCACCAGGGCCTGTAGCCAAGGAGAATTAAAGTCCTGCTCCTGTGATCCAAAG
  AAGAAAGGAAGTGCCAAGGACAGCAAAGGCACCTTCGACTGGGGTGGCTGCAGTGACAATATTGACTACG
  GGATCAAGTTTGCCCGTGCCTTTGTAGATGCCAAGGAGAGGAAAGGCAAGGATGCCAGAGCCCTGATGAA
  CCTTCACAACAACAGAGCTGGAAGGAAGGCTGTAAAGCGCTTCTTGAAACAAGAATGCAAGTGTCATGGT
  GTGAGTGGCTCCTGTACTCTGAGGACATGCTGGCTGGCCATGGCTGACTTCAGGAAAACAGGCGACTATC
  TCTGGAGGAAGTACAATGGGGCCATCCAGGTAGTCATGAACCAGGATGGCACTGGCTTCACTGTAGCCAA
  TAAGAGGTTTAAGAAGCCAACGAAAAATGACCTCGTGTATTTTGAGAATTCTCCAGACTACTGTATCAGG
  GACCGAGAGGCAGGCTCCCTGGGTACAGCGGGCCGTGTGTGCAACTTGACTTCCCGAGGCATGGACAGCT
  GCGAAGTTATGTGTTGTGGGAGAGGCTATGACACATCCCACGTCACCCGGATGACCAAGTGTGAGTGTAA
  ATTCCACTGGTGCTGTGCCGTGCGCTGTCAGGACTGCCTGGAGGCCCTGGACGTGCACACATGCAAGGCC
  CCCAAGAGTGCCGACTGGGCGACGCCTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205513 representing NM_023653
  Red=Cloning site Green=Tags(s)
  
  MNVPLGGIWLWLPLLLTWLTPEVSSSWWYMRATGGSSRVMCDNVPGLVSRQRQLCHRHPDVMRAIGLGVA
  EWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGELKSCSCDPK
  KKGSAKDSKGTFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHG
  VSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANKRFKKPTKNDLVYFENSPDYCIR
  DREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCECKFHWCCAVRCQDCLEALDVHTCKA
  PKSADWATPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023653
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023653.3, NP_076142.2
• RefSeq Size: 2027 bp
• RefSeq ORF: 1083 bp
• Locus ID: 22413
• UniProt ID: P21552
• Cytogenetics: 6 8.08 cM
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:19686689). Functions as upstream regulator of FGF10 expression (PubMed:19686689). Plays an important role in embryonic lung development (PubMed:19686689). May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (PubMed:20018874).[UniProtKB/Swiss-Prot Function]