Arid3a (NM_001288626) Mouse Untagged Clone

CAT#: MC228420

Arid3a (untagged) - Mouse AT rich interactive domain 3A (BRIGHT-like) (Arid3a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid3a
• Synonyms: Bright; Dri1; Dril1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228420 representing NM_001288626
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGCAGGCTGTGATGGAGACTCTCATTCAGAGGCAGCAGCGTGCCCGGCAGGAACTGGAGGCTC
  GGCAGGCTCCACCACCCCCACCACCTGAGCCCACTGGAGTCCGGGCACGGACCACCATGACAGATGAGGA
  CAGGGAGCCTGAGAATGCCCGGATGCATAGGACTCAGATGGCCGCACTGGCTGCCATGCGAGCTGCTGCT
  GCCGGCTTGGGACATCCGTCCTCTCCCGGGGGCTCTGAGGATGGGCCTCCCATCTCTGGGGATGAAGACA
  CAGCCCGGGAAGGGACTCTGAGTTCACCTGCCCTGCATGGAAGTGTCCTGGAGGGAGCAGGACACGCTGA
  GGGAGATAGGCATTTGATGGACGTGGGCTCTGATGATGATGACACGAAGTCCAAGTGGGAAGAGCAAGAG
  CTGGAAGAACTGGGGGAGGAGGAAGAGGAGGAGGAAGAGGAAGATGACTTTGAAGAAGAGGAGGAGGAAG
  AAGAAGGCCTGGGCCCCCCAGAGTCTGCCAGCCTGGGCACTGCAGGCCTGTTCACCCGCAAGGCCCCGCC
  CGCCCAGGCTTTCCGTGGAGACGGTGGTCCCAGGATGCTGAGTGGCCCTGAGCGTCTGGGACCTGGCCCA
  GCCCACCCCAGTCATATGGCATCCCAGATGCCACCACCGGACCATGGGGACTGGACCTTTGAGGAGCAGT
  TCAAACAGCTCTATGAACTGGATGCGGACCCCAAGAGGAAAGAGTTCCTGGATGACCTTTTCAGCTTCAT
  GCAGAAGCGGGGCACTCCAGTGAACCGGATCCCCATCATGGCGAAGCAGGTCCTCGACCTGTTCATGTTG
  TATGTGCTGGTGACCGAGAAGGGCGGCCTGGTAGAGGTTATCAACAAGAAACTGTGGAGGGAGATCACCA
  AGGGGCTCAACCTGCCTACCTCCATCACCAGTGCTGCCTTCACACTTCGGACACAGTACATGAAGTATCT
  TTACCCCTATGAGTGTGAGAGGCGAGGCCTGAGCAGCCCCAACGAGCTCCAGGCCGCCATAGACAGCAAT
  CGCAGGGAGGGCAGGCGCCAGAGCTTCGGTGGCTCACTCTTTGCCTACTCACCCAGTGGAGCCCACAGCA
  TGCTGCCCTCACCCAAGCTACCGGTGACTCCCCTGGGCCTCGCTGCCAGCACCAATGGCAGTTCCATCAC
  CCCAGCGCCCAAGATCAAGAAAGACTCGGCCATCCCCATCACAGTCCCAGGCCGCCTACCTGTATCTTTG
  GCAGGCCACCCTGTTGTCGCAGCCCAGGCTGCGGCGGTGCAAGCTGCGGCAGCCCAAGCAGCCGTTGCGG
  CCCAGGCAGCCGCCCTGGAGCAACTCCGGGAGAAGCTGGAATCCACAGAGCCTCCAGAAAAGAAGATGGC
  CCTAGTGGCCGATGAGCAACAGCGACTCATGCAGCGAGCTGTGCAGCAGAGCTTCCTGGCCATGACAGCC
  CAGCTGCCCATGAACATCCGCATCAACAGCCAAGCCTCTGAGAGCCGCCAGGACTCAGCCGTGAGTCTCA
  CCAGTGCCAACGGGAGCAACAGTATTAGCATGTCCGTGGAGATGAATGGTATTGTATACACAGGTGTGTT
  GTTCGCTCAGCCACCACCTCCTACAGCACCTTCCGCCCCCGGCAAAGGGGGCGTCAGCAGCATCGGTACC
  AACACCACCACGGGCAGCCGGACAGGAGCCAGTGGCAGCACTGTCAGCGGCGGCCAGGTGGGGCTGCCGG
  GGGTGTCTACACCCACCATGTCTTCTACCTCAAACAACTCCTTGCCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288626
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288626.1, NP_001275555.1
• RefSeq Size: 5355 bp
• RefSeq ORF: 1800 bp
• Locus ID: 13496
• UniProt ID: Q62431
• Cytogenetics: 10 C1
• Gene Summary: Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 2 aa shorter compared to isoform a.