Esr1 (NM_001302533) Mouse Untagged Clone

CAT#: MC228048

Esr1 (untagged) - Mouse estrogen receptor 1 (alpha) (Esr1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Esr1
• Synonyms: E; ER; ER-; ER-alpha; ERa; ERalpha; ER[; Es; ESR; Estr; Estra; Nr; Nr3a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228048 representing NM_001302533
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGACCCTTCACACCAAAGCCTCGGGAATGGCCTTGCTGCACCAGATCCAAGGGAACGAGCTGG
  AGCCCCTCAACCGCCCGCAGCTCAAGATGCCCATGGAGAGGGCCCTGGGCGAGGTATACGTGGACAACAG
  CAAGCCCACTGTGTTCAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCCGCCGCCGCCGCCGCC
  GCCGCCTCGGCGCCGGTCTACGGCCAGTCGGGCATCGCCTACGGCCCCGGGTCGGAGGCGGCCGCCTTCA
  GTGCCAACAGCCTGGGGGCTTTCCCCCAGCTCAACAGCGTGTCGCCTAGCCCGCTGATGCTGCTGCACCC
  GCCGCCGCAGCTGTCTCCTTTCCTGCACCCGCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCC
  AGCGCCTACGCCGTGCGCGACACCGGCCCTCCCGCCTTCTACAGGTCTAATTCTGACAATCGACGCCAGA
  ATGGCCGAGAGAGACTGTCCAGCAGTAACGAGAAAGGAAACATGATCATGGAGTCTGCCAAGGAGACTCG
  CTACTGTGCCGTGTGCAATGACTATGCCTCTGGCTACCATTATGGGGTCTGGTCCTGCGAAGGCTGCAAG
  GCTTTCTTTAAGAGAAGCATTCAAGGACACAATGACTACATGTGTCCAGCTACAAACCAATGCACCATTG
  ACAAGAACCGGAGGAAGAGTTGCCAGGCCTGTCGGCTGCGCAAGTGTTACGAAGTGGGCATGATGAAAGG
  CGGCATACGGAAAGACCGCCGAGGAGGGAGAATGTTGAAGCACAAGCGTCAGAGAGATGACTTGGAAGGC
  CGAAATGAAATGGGTGCTTCAGGAGACATGAGGGCTGCCAACCTTTGGCCAAGCCCTCTTGTGATTAAGC
  ACACTAAGAAGAATAGCCCTGCCTTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTGTTGGATGCTGA
  ACCGCCCATGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGCTTATTG
  ACCAACCTAGCAGATAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTGGGGACT
  TGAATCTCCATGATCAGGTCCACCTTCTCGAGTGTGCCTGGCTGGAGATTCTGATGATTGGTCTCGTCTG
  GCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCCTGGACAGAGAACATTCTGTC
  AAGACTTTGAAAAGAAGCTCTGACAAGGACCTACAAGGACTTCAGACAACTGGGATTCCTTTATGTCAGA
  AAGAAGATGGACTACTTTTAAAAAATGCAACCAGGGTACAAGAAAAACCAACCTGTAGTCTGCACTCTCT
  AACCAAGGCCAAGAGAGCCTGCCATGTGATACTAGTCCCATCCCCAGAGTCCAGAAATGTAGGTGTCAAA
  GATTCCACTCTGCTCGTAGTGAACTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302533
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302533.1, NP_001289462.1
• RefSeq Size: 2730 bp
• RefSeq ORF: 1500 bp
• Locus ID: 13982
• Cytogenetics: 10 2.03 cM
• Gene Summary: This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (4) differs in the 5' UTR, contains an alternate exon in the 3' coding region and uses an upstream alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.