Hnrnpk (NM_001301345) Mouse Untagged Clone

CAT#: MC227707

Hnrnpk (untagged) - Mouse heterogeneous nuclear ribonucleoprotein K (Hnrnpk), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hnrnpk
• Synonyms: hn; Hnrpk; KBB; KBBP; NOVA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227707 representing NM_001301345
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCGAACAGCCAGAAGAAACCTTCCCCAACACCGAAACCAATGGTGAATTTGGTAAACGCCCTG
  CAGAAGATATGGAAGAGGAGCAAGCCTTTAAAAGATCTAGAAATACTGATGAGATGGTTGAATTGCGCAT
  TTTGCTTCAGAGCAAGAATGCTGGAGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACA
  GACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATACTGAGTATCAGTGCTGATA
  TTGAGACGATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGTACCAACATTATAAAGGAAG
  TGACTTTGATTGCGAGTTGAGACTGTTGATTCATCAGAGTCTGGCAGGAGGAATAATTGGTGTTAAAGGT
  GCTAAAATCAAAGAACTTCGAGAAAACACTCAGACAACAATCAAGCTTTTCCAGGAGTGCTGCCCTCACT
  CTACTGACAGAGTTGTTCTTATTGGAGGAAAACCTGATAGGGTTGTAGAATGCATCAAGATCATCCTTGA
  CCTTATATCTGAGTCTCCCATCAAAGGACGTGCACAACCTTATGATCCCAACTTTTATGATGAGACCTAT
  GATTATGGTGGTTTTACAATGATGTTTGATGACCGCCGAGGACGACCTGTGGGATTCCCCATGAGGGGAA
  GAGGTGGTTTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCTTCTAGAAGAGATTATGA
  TGATATGAGCCCTCGTCGAGGACCTCCACCACCACCACCTGGTCGAGGTGGCCGGGGTGGCAGCAGAGCC
  CGGAATCTGCCTCTTCCTCCTCCACCACCACCCAGAGGGGGAGATCTAATGGCTTATGACAGAAGAGGAA
  GGCCTGGAGACCGCTATGATGGCATGGTTGGGTTCAGTGCTGATGAAACTTGGGATTCTGCAATTGACAC
  ATGGAGCCCATCAGAATGGCAAATGGCTTATGAACCACAGGGTGGTTCTGGATATGACTATTCTTATGCA
  GGGGGCCGTGGCTCATATGGTGATCTTGGCGGACCTATTATCACTACACAAGTAACTATTCCCAAAGATT
  TGGCTGGATCTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATTCGTCATGAATCTGGAGCATCAAT
  CAAAATTGATGAACCTTTAGAAGGATCTGAAGATCGGATCATTACCATTACAGGAACACAGGACCAGATA
  CAGAACGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATTCTGGAAAGTTTTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301345
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301345.1, NP_001288274.1
• RefSeq Size: 2721 bp
• RefSeq ORF: 1320 bp
• Locus ID: 15387
• UniProt ID: P61979
• Cytogenetics: 13 B1
• Gene Summary: The protein encoded by this gene is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex, and is a poly-cytosine binding protein (PCBP). It is found in multiple subcellular compartments including the nucleus, cytoplasm and mitochondria. This gene product is thought to interact with RNA, DNA, and protein, and is involved in multiple cellular processes, including transcription, chromatin remodeling, DNA damage response, signal transduction, mRNA splicing, export, and translation. Multiple transcript variants and protein isoforms exist, with some isoforms containing a unique C-terminus. There are four pseudogenes of this gene, found on chromosomes 2, 3, 7 and 13. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (6) lacks an in-frame exon and uses an alternative splice site in the 3' terminal exon, which results in a frameshift, compared to variant 1. It encodes isoform 4 which is shorter and has a distinct C-terminus compared to isoform 1.