Dbf4 (NM_001190717) Mouse Untagged Clone

CAT#: MC220247

Dbf4 (untagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dbf4
• Synonyms: AA545217; Ask
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220247 representing NM_001190717
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCGGGTGGCATCCAAGACAGAAATGAAA
  AGAATCGACCATCCTTGAAATCCCTGAAGGCTGATAATAGACTGGAAAAATCCAAATATAAGCCCCTTTG
  GGGAAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAGAAACTACAAAAAGATATAAAGGAA
  CTCGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATTTTGTTTCAAATAAAAAGGAAGCAA
  AGTATGCACAGACATTGGGTCGCGTTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC
  CTCTCCTCATCCCAGCCATGATGGAAGTTCCTTTAAGTCTCAAGACAGAGTATGTTTGAGCCGAGGAAAA
  TTGTTAGCTGAAAAAGCTGTGAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAATGCCTTAT
  CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGATACTACATTGAACAGAAGAAGAAAGCACTGAG
  TGCACTCAAGAAATCCAGTGCTTCTGGGAAGGATGCGGGGAAAAAAGCTGGTCCTGGAATACAAAAGACA
  AGAAGGAGACTCAAGAAGCCTTTTTTAAAGGTTGAAGATGTGAACCGGTGCTACAGGCCATTTTACCTTC
  AGCTCCCCAGTCTGCCGTGTATAAACTACTTTCTTCAGAAGCCCTGCAGTCCGTTTGATATAGAGAAGTC
  ATCCAGCGTCCAGAAACAAGCTCAGCCCAAGCTCAGAATTAATATGGACGGCGATAAATGTGGAACCCCA
  GTTCAACTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTACAGAAATACGAAGATC
  TCGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGATGA
  TATTGTATCTCAGTTAGTTTTCGATTTTGTGGAATATGGAAGAGACACACCTCAAAAGAAAAGAATAAGA
  TACAGTGTCGGGTCCCTTTCTTCTGTTTCTGCAAATGTCCTGAAAAACACTGCACCGAAGGAGAAGCCAC
  TCTTGGAGCCCAATTTCCAGAAGGACGTGGGCGAAAGCAGTGGGCACCTGCTCAAGCCGAACTCTCAGTA
  TGAAGAAACCCAGAAACCTGAAGAAAAGCATGGGTTTGCTTCAGAGCCCACAACCTATTCTTCAGCTGGA
  TTGAAAGGATGTGACAGGAAACCAGTGAGCATGTTCAATGCAAGTGAGCCTGACCCAGAGCAGGAGTATG
  CACAGCTACCTCTGCGTGACAGCACACCGGAACACCAAGTGACCGAGGGTAGAAATGACGGAGAACAGAG
  AGTAGACCCTGCTCCAGGCGTGTCGCAGTCATGTGGACAAGTTTCTCATCTCAGTACAGAAAGTAATCTA
  CCCCAACCACAGTTAGCTGCAGACATTACACAGCTTTCAGCAAAAGATCTGCAAGAAAAGGGCTTTCATG
  TTGTGATAGGTCATGCTTCTGACCTGGTGGCATTAAATACATCAAAAGAGCAGCTAACAATGAAGGCGAG
  AACTCCACCCTGTAGTCCCCAGGAGCCTCATGAGTGTGACACTGAGAACATGGAGAATTTGCCTTGTGGT
  AAAATCCAGCGGAAAGTGAGAATGCTATTAGGGCAACAGAAAGCAAATGCTGAACCGAGTGCTGAATTGG
  ATAAAAAGAGAACTGAGTATCTTCCTGCACATGAAGACAGAACTTGTGGGTCACCAGTACAGTCTCTATT
  GGACCTTTTTCAGACAAGCGAGGAGAAATCAGAATTTTTGGGTTTTACAGGCTACACTGAAAACAGTGGA
  ATATGTGATGTTTTAGATATTTGGGAGGAAGAAAATTCAAGTACCCTGTTGTCAACGTTTTTTTCTTCCC
  CTTCAACTTCTGCATTCGTTGGATTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190717
• Insert Size: 1989 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190717.1, NP_001177646.1
• RefSeq Size: 2441 bp
• RefSeq ORF: 1989 bp
• Locus ID: 27214
• UniProt ID: Q9QZ41
• Cytogenetics: 5 A1
• Gene Summary: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.