Esr2 (NM_010157) Mouse Untagged Clone

CAT#: MC218802

Esr2 (untagged) - Mouse estrogen receptor 2 (beta) (Esr2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Esr2
• Synonyms: ERbeta; ER[b]; Estrb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218802 representing NM_010157
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCTGTGCCTCTTCTCACAAGGATTTTTCTCAGCTGAGACCTACGCAAGACATGGAGATCAAAA
  ACTCACCATCAAGCCTTACTTCCCCTGCTTCTTATAACTGTAGCCAGTCCATCCTACCCTTGGAGCATGG
  TCCCATCTATATCCCTTCCTCCTATGTAGAGAGCCGTCACGAATACTCAGCCATGACATTCTACAGTCCT
  GCTGTGATGAACTACAGTGTTCCCAGCAGCACCGGTAACCTGGAAGGTGGGCCTGTTCGCCAGACTGCAA
  GCCCAAATGTGCTATGGCCAACTTCTGGACACCTCTCTCCTTTAGCCACCCACTGCCAATCATCGCTTCT
  CTATGCAGAACCTCAAAAGAGTCCTTGGTGTGAAGCAAGATCACTAGAACACACCTTGCCTGTAAACAGA
  GAGACCCTGAAGAGGAAGCTTGGCGGGAGCGGTTGTGCCAGCCCTGTTACTAGTCCAAGCGCCAAGAGGG
  ATGCTCACTTCTGCGCCGTCTGCAGTGATTATGCATCTGGGTATCATTACGGTGTCTGGTCCTGTGAAGG
  ATGTAAGGCCTTTTTTAAAAGAAGCATTCAAGGACATAATGACTATATCTGTCCAGCCACGAATCAGTGT
  ACCATAGACAAGAACCGGCGTAAAAGCTGCCAGGCCTGCCGACTTCGCAAGTGTTACGAAGTAGGAATGG
  TCAAGTGTGGATCCAGGAGAGAAAGGTGTGGGTACCGAATAGTACGAAGACAGAGAAGTGCCAGCGAGCA
  GGTGCATTGCCTGAACAAAGCCAAGAGAACCAGTGGGCACACACCCCGGGTGAAGGAGCTACTGCTGAAC
  TCTCTGAGTCCCGAGCAGCTGGTGCTCACCCTGCTGGAAGCTGAGCCACCCAATGTGCTAGTGAGCCGTC
  CCAGCATGCCCTTCACCGAGGCCTCCATGATGATGTCCCTCACGAAGCTGGCTGACAAGGAACTGGTGCA
  CATGATTGGCTGGGCCAAGAAAATCCCTGGCTTTGTGGAGCTCAGCCTGTTGGACCAAGTCCGCCTCTTG
  GAAAGCTGCTGGATGGAGGTGCTGATGGTGGGGCTGATGTGGCGCTCCATCGACCACCCCGGCAAGCTCA
  TCTTTGCTCCAGACCTCGTTCTGGACAGGGATGAGGGGAAGTGCGTGGAAGGGATTCTGGAAATCTTTGA
  CATGCTCCTGGCGACGACGGCACGGTTCCGTGAGTTAAAACTGCAGCACAAAGAATATCTGTGTGTGAAG
  GCCATGATTCTCCTCAACTCCAGTATGTACCCCTTGGCTACCGCAAGCCAGGAAGCAGAGAGTAGCCGGA
  AGCTGACACACCTATTGAACGCAGTGACAGATGCCCTGGTCTGGGTGATTTCGAAGAGTGGAATCTCTTC
  CCAGCAGCAGTCAGTCCGTCTGGCCAACCTCCTGATGCTTCTTTCTCATGTCAGGCACATCAGTAACAAG
  GGCATGGAACATCTGCTCAGCATGAAGTGCAAAAATGTGGTCCCGGTGTACGACCTGCTGCTGGAGATGC
  TGAATGCTCACACGCTTCGAGGGTACAAGTCCTCAATCTCGGGGTCTGAGTGCTGCTCGACAGAGGACAG
  TAAGAGCAAAGAGGGCTCCCAGAACCTCCAGTCACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010157
• Insert Size: 1650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010157.3, NP_034287.3
• RefSeq Size: 3308 bp
• RefSeq ORF: 1650 bp
• Locus ID: 13983
• UniProt ID: O08537
• Cytogenetics: 12 33.52 cM
• Gene Summary: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1 (ER-alpha), and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. May play a role in ovarian follicular growth and maturation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.