Hsd11b1 (NM_008288) Mouse Untagged Clone

CAT#: MC208710

Hsd11b1 (untagged) - Mouse hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsd11b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC132364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTATGAAAAATTACCTCCTCCCGATCCTGGTGCTCTTCCTGGCCTACTACTACTATTCTACAA
  ATGAAGAGTTCAGACCAGAAATGCTCCAGGGAAAGAAAGTGATTGTCACTGGGGCCAGCAAAGGGATTGG
  AAGAGAAATGGCATATCATCTGTCAAAAATGGGAGCCCATGTGGTATTGACTGCCAGGTCGGAGGAAGGT
  CTCCAGAAGGTAGTGTCTCGCTGCCTTGAACTCGGAGCAGCCTCTGCTCACTACATTGCTGGCACTATGG
  AAGACATGACATTTGCGGAGCAATTTATTGTCAAGGCGGGAAAGCTCATGGGCGGACTGGACATGCTTAT
  TCTAAACCACATCACTCAGACCTCGCTGTCTCTCTTCCATGACGACATCCACTCTGTGCGAAGAGTCATG
  GAGGTCAACTTCCTCAGCTACGTGGTCATGAGCACAGCCGCCTTGCCCATGCTGAAGCAGAGCAATGGCA
  GCATTGCCGTCATCTCCTCCTTGGCTGGGAAAATGACCCAGCCTATGATTGCTCCCTACTCTGCAAGCAA
  GTTTGCTCTGGATGGGTTCTTTTCCACCATTAGAACAGAACTCTACATAACCAAGGTCAACGTGTCCATC
  ACTCTCTGTGTCCTTGGCCTCATAGACACAGAAACAGCTATGAAGGAAATCTCTGGGATAATTAACGCCC
  AAGCTTCTCCCAAGGAGGAGTGCGCCCTGGAGATCATCAAAGGCACAGCTCTACGCAAAAGCGAGGTGTA
  CTATGACAAATCGCCTTTGACTCCAATCCTGCTTGGGAACCCAGGAAGGAAGATCATGGAATTTTTTTCA
  TTACGATATTATAATAAGGACATGTTTGTAAGTAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008288
• Insert Size: 879 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC132364, AAI32365
• RefSeq Size: 1141 bp
• RefSeq ORF: 879 bp
• Locus ID: 15483
• UniProt ID: P50172
• Cytogenetics: 1 H6
• Gene Summary: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. An in-frame AUG is located 41 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context.