Actb (NM_007393) Mouse Untagged Clone

CAT#: MC208102

Actb (untagged) - Mouse actin, beta (Actb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Actb
• Synonyms: Act; Actx; beta-a; beta-actin; E430023M04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208102 representing NM_007393
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACGATATCGCTGCGCTGGTCGTCGACAACGGCTCCGGCATGTGCAAAGCCGGCTTCGCGGGCG
  ACGATGCTCCCCGGGCTGTATTCCCCTCCATCGTGGGCCGCCCTAGGCACCAGGGTGTGATGGTGGGAAT
  GGGTCAGAAGGACTCCTATGTGGGTGACGAGGCCCAGAGCAAGAGAGGTATCCTGACCCTGAAGTACCCC
  ATTGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAATGAGC
  TGCGTGTGGCCCCTGAGGAGCACCCTGTGCTGCTCACCGAGGCCCCCCTGAACCCTAAGGCCAACCGTGA
  AAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCATGTACGTAGCCATCCAGGCTGTGCTG
  TCCCTGTATGCCTCTGGTCGTACCACAGGCATTGTGATGGACTCCGGAGACGGGGTCACCCACACTGTGC
  CCATCTACGAGGGCTATGCTCTCCCTCACGCCATCCTGCGTCTGGACCTGGCTGGCCGGGACCTGACAGA
  CTACCTCATGAAGATCCTGACCGAGCGTGGCTACAGCTTCACCACCACAGCTGAGAGGGAAATCGTGCGT
  GACATCAAAGAGAAGCTGTGCTATGTTGCTCTAGACTTCGAGCAGGAGATGGCCACTGCCGCATCCTCTT
  CCTCCCTGGAGAAGAGCTATGAGCTGCCTGACGGCCAGGTCATCACTATTGGCAACGAGCGGTTCCGATG
  CCCTGAGGCTCTTTTCCAGCCTTCCTTCTTGGGTATGGAATCCTGTGGCATCCATGAAACTACATTCAAT
  TCCATCATGAAGTGTGACGTTGACATCCGTAAAGACCTCTATGCCAACACAGTGCTGTCTGGTGGTACCA
  CCATGTACCCAGGCATTGCTGACAGGATGCAGAAGGAGATTACTGCTCTGGCTCCTAGCACCATGAAGAT
  CAAGATCATTGCTCCTCCTGAGCGCAAGTACTCTGTGTGGATCGGTGGCTCCATCCTGGCCTCACTGTCC
  ACCTTCCAGCAGATGTGGATCAGCAAGCAGGAGTACGATGAGTCCGGCCCCTCCATCGTGCACCGCAAGT
  GCTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007393
• Insert Size: 1128 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138611, AAI38612
• RefSeq Size: 1584 bp
• RefSeq ORF: 1128 bp
• Locus ID: 11461
• UniProt ID: P60710
• Cytogenetics: 5 81.8 cM
• Gene Summary: This gene encodes a member of the actin family of proteins. Actins are highly conserved proteins that are among the most abundant proteins in eukaryotic cells and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the transcribed mRNA are regulated through the binding of multiple factors to its 3' UTR sequence. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous pseudogenes of this gene have been identified in the mouse genome. [provided by RefSeq, Sep 2015]