Upb1 (NM_133995) Mouse Untagged Clone

CAT#: MC207804

Upb1 (untagged) - Mouse ureidopropionase, beta (Upb1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Upb1
• Synonyms: AI195023
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207804 representing NM_133995
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGACCGGAGTGGCAGTCCCTGGAGCAGTGCCTGGAAAAACATCTGCCGCCTGACGATTTGGCCC
  AGGTGAAGCGAATTCTCTACGGCAAGCAGACTAGGAATCTCGATCTGCCCAGAGAAGCTTTAAAAGCTGC
  CTCAGAAAGGAACTTTGAACTGAAGGGATATGCCTTCGGGGCAGCCAAGGAGCAGCAGAGATGCCCCCAG
  ATCGTGCGCGTGGGGCTCGTTCAGAACAGAATCCCGCTCCCCACTTCTGCCCCCGTGGCAGAACAGGTCT
  CTGCGCTCCACAAGAGCATAGAGGAGATTGCTGAGGTGGCCGCAATGTGTGGAGTCAATATCATCTGTTT
  CCAGGAAGCATGGAATATGCCGTTCGCTTTTTGTACACGAGAGAAACTTCCTTGGACAGAATTTGCTGAG
  TCGGCCGAGGATGGGCTCACCACCAGGTTCTGTCAGAAGCTGGCAAAGAAGCACAACATGGTGGTGGTGT
  CTCCAATCTTGGAGCGTGATAGAGAGCATGGGGGAGTTCTGTGGAACACAGCTGTGGTGATCTCCAATTC
  AGGGCTGGTCATGGGGAAGACCAGAAAGAACCACATCCCCAGAGTGGGAGACTTCAACGAGTCCACTTAC
  TACATGGAGGGAAACCTGGGCCACCCCGTGTTCCAGACCCAGTTTGGCAGGATTGCAGTGAACATTTGCT
  ATGGGCGGCACCACCCCCTCAACTGGCTCATGTACAGCATCAACGGAGCTGAAATCATCTTCAACCCTTC
  GGCCACCATTGGAGAACTCAGTGAGTCCTTGTGGCCGATCGAAGCCAGAAATGCAGCCATTGCCAATCAC
  TGCTTCACCTGTGCTCTCAACCGTGTGGGTCAGGAACACTTCCCCAATGAGTTTACTTCTGGAGATGGAA
  AGAAAGCTCACCATGACCTCGGCTACTTCTATGGTTCGAGCTATGTGGCAGCTCCCGATGGCAGCCGGAC
  CCCCGGCCTCTCCCGTAATCAGGATGGGCTGCTGGTCACGGAGCTCAACCTCAATCTCTGCCAGCAGATC
  AATGATTTCTGGACTTTCAAGATGACGGGACGACTTGAGATGTATGCCCGGGAACTTGCCGAAGCGGTCA
  AACCCAACTACAGTCCCAACATTGTGAAGGAAGACCTGGTACTAGCCCCTAGCTCGGGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133995
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133995.4, NP_598756.1
• RefSeq Size: 3042 bp
• RefSeq ORF: 1182 bp
• Locus ID: 103149
• UniProt ID: Q8VC97
• Cytogenetics: 10 C1
• Gene Summary: This gene encodes an enzyme that catalyzes the conversion of N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to beta-aminoisobutyric acid or beta-alanine. Mutations in the related gene in human have been associated with beta-ureidopropionase deficiency. [provided by RefSeq, Dec 2013]