Idh3a (NM_029573) Mouse Untagged Clone

CAT#: MC202282

Idh3a (untagged) - Mouse isocitrate dehydrogenase 3 (NAD+) alpha (Idh3a), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Idh3a
• Synonyms: 1110003P10Rik; 1500012E04Rik; AA407078; AI316514
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049956 sequence for NM_029573
  GACGCGATGGCCGGGTCCGCGTGGGTGTCCAAGGTCTCTCGGCTGCTGGGTGCATTCCACAACACAAAAC AGGTGACAAGAGGTTTTGCTGGTGGTGTTCAGACAGTAACTTTAATTCCTGGAGATGGAATTGGCCCAGA AATTTCAGCCTCAGTCATGAAGATTTTTGATGCTGCCAAAGCACCTATTCAGTGGGAGGAGCGCAATGTC ACAGCAATTCAAGGACCAGGAGGAAAGTGGATGATCCCTCCAGAAGCCAAGGAGTCCATGGATAAGAACA AGATGGGCTTGAAAGGCCCACTAAAGACCCCAATAGCCGCTGGCCATCCATCTATGAATCTGTTGCTTCG TAAGACATTTGACCTTTATGCCAATGTCCGGCCATGTGTCTCAATTGAAGGTTATAAAACCCCTTACACG GATGTAAATATCGTCACCATCCGAGAGAACACGGAAGGAGAATACAGTGGAATTGAGCATGTGATCGTTG ATGGGGTTGTGCAGAGCATCAAGCTCATCACCGAAGAAGCAAGCAAGCGCATTGCAGAGTTTGCCTTCGA GTACGCTCGGAACAACCACCGGAGCAACGTCACAGCTGTGCACAAAGCTAACATCATGAGGATGTCAGAT GGGCTCTTTCTGCAAAAATGCAGGGAAGTTGCGGAGAACTGTAAAGACATTAAATTTAACGAGATGTACC TTGATACTGTATGTTTAAATATGGTACAAGACCCATCCCAGTTTGATGTTCTTGTCATGCCAAATTTATA CGGAGACATCCTTAGTGATCTGTGTGCAGGACTGATTGGAGGTCTTGGGGTGACTCCAAGTGGCAATATT GGAGCCAACGGTGTTGCCATCTTTGAATCGGTTCATGGAACAGCCCCGGACATTGCAGGCAAGGACATGG CCAACCCCACGGCCCTCCTGCTTAGTGCTGTGATGATGCTTCGCCACATGGGACTTTTTGACCATGCAGC AAAAATCGAGGCTGCATGTTTTGCTACAATTAAGGATGGAAAGAGCTTAACAAAAGATCTGGGAGGCAAC GCGAAGTGCTCTGACTTCACAGAAGAAATCTGTCGTAGAGTCAAAGACTTAGATTAGCACTCCTGCTGGT GGATTTGCTGCAGTCAGTCAATCACTCCAAAAGGATACCCTGTAATCCTCCTTGAGGGCGCCCACCATTG GTTTGCTTGCTTCTTGACAGAGTACGTTTTTTGAATCTGGCCTTTTCTTAACAAAACCCTTGCAATGGAT GCACATGATGGCCCCAGGCCTTCATTCAAAGGGTTTTCCCAAGTGCTGGTTGTATTTATTGTCCGTCTGG TAAACCTTATTTTGTAAACTGTAAGTGAACTGTATCATTTATCATTGTTAACCCATTTTACACTTCAGGC AAAATCATTTTCCTCAACTGTAAATATTCTGATACAGAATTAATAAGAGAAGATATTTAACTTTTTAACA AAAGCCCTGGATTTTTGGTTTATGAAAAACAAACTGGGAATAAAACAGGGTTTTAACAATCGCACAAGAT AACATTATTCTAATACTAATGGGTACAAAAGAAATTTACTGGGAAAGTTCACAGCAAAAAAATGGTATAT TTCTTAAAAATATGGAAATAAAGTATTTGTCCTATACATGAATTACTATTAATAAAAATGTAAGCTCCAA GAAATCCATAATGAATGATGTAATTTTGTTACTACATCGGTAATCCTTGTCAAGGCCCCGGATGCTCTCT GTGTATTTGATTCTTTTGTTACCTTGAGATTCACTATTTTGGGGGAAGAGCTTTCAGATAAGGGAGATCA CTCCTCACTAGACAGATCGTCAGCATTGCGAGCTGTCAGCCATGAGAGCCAGCCACTGCAGATCCCCTCC CACGTGGCCACACTCCAGCCAGTGCTGCAGGTGACCCTGGAAAGGCCTGGCTGCCCCTTGACTTTCCCTA AAGCAACCAGTCACTGCCTTCTGCCCCAGTAGCACCCATTACAGACTTAATTGCCGAGGTGGAGCTGACT CAGCCCACGCTCATACAAATCAGGCCAAGCGGGGGCCTGTGTTACCAGCTGCTGACCATCAGGTTCTGCC CCTCATTCTTCCCACAGCCTCTGCTCCACAGCATGAACCTAGCCTTTGGCCCACACCAAAGCCAAGCTGT CTTCCCTTAGCCCTTGCACTAGTTTGCAAACTCGTGGCTTTGCATAATGTACCCTGGTCCCAAGGGGATT TCTTAACAACAGATGTCCCTGTCTGGGTCATTTTTTTAAAGCTTTTATTTGGACTTACAATCTTCTGTGT ATTTTACTTTAAAACTGCTGCTTTCCCTGTCTCACTGGATTGTTCTGGTTAGCAGTGGCTTTGGGTTCAC AGTAATAAAGAACTTAAGAACTGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029573
• Insert Size: 1101 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049956, AAH49956
• RefSeq Size: 2418 bp
• RefSeq ORF: 1101 bp
• Locus ID: 67834
• UniProt ID: Q9D6R2
• Cytogenetics: 9 A5.3
• Gene Summary: Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.[UniProtKB/Swiss-Prot Function]