Egln2 (NM_053208) Mouse Untagged Clone

CAT#: MC201719

Egln2 (untagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Egln2
• Synonyms: 0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023299 sequence for NM_053208
  AGCGCAGGGCGGCTGGCACAAACGGCGGCGCCGAGGCCGGAGGAAAAAGCTCGCCACCCCCATCAGTCCC TTCTCAAGCTCCTAGAGACAACTGTGGACTTGGGGACCAGCGAGCACCCCCAGAGCACTAGAGGAGCCCC TGCTGCCTGCCCTGCCTCACCCTGCCCCACACGAGGCCCAGCGGCCCCGGGCTGCATCAAGTGGAGGAGG AGGAGGCGGAGGAGGGTGGCACCATGGGCCCGGGCCGTGCCCTCCATGCCCGGGGGATGAAGACACTGCT GCCATGGACAGCCCGTGCCAGCCGCAGGCCCTGAATCAAGCTCTCCCTCAGTTGCCAGGGTCTGTGTCAG AGTCCTTGGAGTCTAGCCGAGCCAGAATGGGGGTGGAGAGTTACCTGCCCTGCCCTCTGCTCCCGGCCTA TCACCGTCCAGGAGCATCTGGGGAGGCCTCGGCTGGCAATGGGACCCCCAGAACCACAGCCACTGCTACT ACGACCACTGCCAGTCCCCTGCGGGAGGGCTTTGGTGGGCAGGATGGTGGTGAGCTTTGGCCACTGCAGA GTGAAGGTGCTGCTGCGTTGGTCACCAAGGAGTGCCAGCGACTGGCGGCCCAGGGTGCCCGGCCTGAGGC CCCCAAACGGAAGTGGGCCAAGGATGGTGGGGATGCCCCTTCACCCAGCAAGCGACCGTGGGCCAGGCAA GAGAACCAGGAGGCCAAGGGGGAAAGTGGTATGGGCTGTGACAGCGGTGCCAGCAACAGCAGCAGCAGCA GCAGCAACACTACCAGTAGCAGTGGCGAGGCAAGTGCTAGGCTGAGGGAGGAAGTCCAGCCCTCTGCACC TGAGCGCCTGGCCCTGGACTATATTGTGCCTTGCATGCGGTACTATGGTATCTGTGTCAAGGACAACTTC TTGGGGGCAGTACTGGGTGGCCGTGTGCTGGCTGAGGTGGAAGCCCTGAAGTGGGGCGGGCGTCTTCGTG ATGGGCAACTAGTGAGCCAGCGGGCGATCCCACCGCGCAGCATTCGTGGGGACCAGATTGCCTGGGTAGA AGGTCACGAGCCAGGCTGCCGGAGCATTGGTGCCCTCATGGCTCACGTGGACGCAGTAATCCGCCACTGT GCAGGGCGGCTGGGCAACTACGTCATCAATGGGCGCACCAAGGCCATGGTGGCGTGTTATCCAGGCAATG GGCTCGGGTACGTGAGGCATGTTGACAATCCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCT GAATCAGAACTGGGATGTTAAGGTGCATGGCGGCCTGCTGCAGATCTTCCCCGAGGGTCGGCCAGTGGTA GCCAACATCGAGCCACTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGACGGAACCCACATGAGGTGA AGCCAGCCTATGCCACCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAACGGGCAGCAGCCAG AGACAAGTATCAGCTAGCATCGGGACAGAAAGGTGTTCAAGTACCAGTATCACAGCCAACTACACCTACC TAATGGCCAGCCCCAGAGCTGCGTGGACCAGCAGCAGCTCCTGCCTCAGTGCCCGCTCCTTTCCTGCCAC TGCTGCTGCTTCTGGCTTGCCTCTGTACTGTGTGGTGTGGAGGGCACTAAGTATCACTGAGGAGCAACAA GGAGAGACCTCTGCTGCCCCGTGAGCGAGCGGTGCTGGGTTTTGACCTGGGCAGTGGCCAGTGTGGCTGG CGGTCATGACTGGTGGCTGTGTCTGGTCCGTTGAGTGTAGAGCTGAGAAGAGGCAGGATTTGGGGTTGAG GTGAGTCATGGCCTCTTGCTGGAAAGGTGTGTTGGGGTGTGGGTGTCATCTCACCCTCGTCCCCACTCCT CCGGCCTGGAATGTGAAGTGACTCCCCAGCCCCTTTGGCTGTGGCAGTGTATGGACTGGGCTGCCACTGT CTGGGCAGAGTAGGGTGCCATGACGAGCATGGGTATGGAGGTTCTGCCAGCCAAGAAATAAAAGTTTACC TCAGAGCTGCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_053208
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023299, AAH23299
• RefSeq Size: 2055 bp
• RefSeq ORF: 1260 bp
• Locus ID: 112406
• UniProt ID: Q91YE2
• Cytogenetics: 7 15.83 cM
• Gene Summary: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function]