Rat Kras (NM_031515) AAV Particle

CAT#: RR212693A1V

AAV ORF Particles, serotype AAV-2, Kras (Myc-DDK-tagged ORF) - Rat v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras), (10 ug), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Kras
• Synonyms: c-Ki-ras; Kras2; p21
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212693 representing NM_031515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGTATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAA
  TTCAGAATCACTTTGTGGATGAATATGATCCTACGATAGAGGACTCCTACAGGAAACAAGTAGTAATTGA
  TGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAG
  TACATGAGAACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTC
  ACCATTATAGAGAACAAATTAAAAGAGTAAAGGACTCTGAAGATGTGCCTATGGTCCTAGTAGGGAATAA
  GTGTGACTTGCCTTCTAGAACAGTAGACACGAAACAGGCTCAGGAGTTAGCAAGGAGTTATGGGATTCCA
  TTCATTGAGACCTCAGCGAAGACAAGACAGGGTGTTGACGATGCCTTCTATACATTAGTCCGAGAAATTC
  GAAAACATAAAGAAAAGATGAGCAAAGATGGGAAAAAGAAGAAGAAGAAGTCAAGGACAAGGTGTATAGT
  CATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212693 representing NM_031515
  Red=Cloning site Green=Tags(s)
  
  MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ
  YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIP
  FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSRTRCIVM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Rat
• Serotype: AAV-2
• ACCN: NM_031515
• ORF Size: 564 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_031515.3, NP_113703.1
• RefSeq Size: 661 bp
• RefSeq ORF: 567 bp
• Locus ID: 24525
• UniProt ID: P08644
• Cytogenetics: 4q44
• MW: 21.5 kDa