Human CYP21A2 (NM_000500) AAV Particle

CAT#: RC216416A1V

AAV ORF Particles, serotype AAV-2, CYP21A2 (Myc-DDK-tagged)-Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: CYP21A2
• Synonyms: CA21H; CAH1; CPS1; CYP21; CYP21B; P450c21B
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216416 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCCTGGGCCTGCTGCTGCTGCTGCCCCTGCTGGCTGGCGCCCGCCTGCTGTGGAACTGGTGGA
  AGCTCCAGAGCCTCCACCTCCCGCCTCTTGCCCCGGGCTTCTTGCACTTGCTGCAGCCCGACCTCCCAAT
  CTATCTGCTTGGCCTGACTCAGAAATTCGGGCCCATCTACAGGCTCCACCTTGGGCTGCAAGATGTGGTG
  GTGCTGAACTCCAAGAGGACCATTGAGGAAGCCATGGTCAAAAAGTGGGCAGACTTTGCTGGCAGACCTG
  AGCCACTTACCTACAAGCTGGTGTCTAGGAACTACCCGGACCTGTCCTTGGGAGACTACTCCCTGCTCTG
  GAAAGCCCACAAGAAGCTCACCCGCTCAGCCCTGCTGCTGGGCATCCGTGACTCCATGGAGCCAGTGGTG
  GAGCAGCTGACCCAGGAGTTCTGTGAGCGCATGAGAGCCCAGCCCGGCACCCCTGTGGCCATTGAGGAGG
  AATTCTCTCTCCTCACCTGCAGCATCATCTGTTACCTCACCTTCGGAGACAAGATCAAGGACGACAACTT
  AATGCCTGCCTATTACAAATGTATCCAGGAGGTGTTAAAAACCTGGAGCCACTGGTCCATCCAAATTGTG
  GACGTGATTCCCTTTCTCAGGTTCTTCCCCAATCCAGGTCTCCGGAGGCTGAAGCAGGCCATAGAGAAGA
  GGGATCACATCGTGGAGATGCAGCTGAGGCAGCACAAGGAGAGCCTCGTGGCAGGCCAGTGGAGGGACAT
  GATGGACTACATGCTCCAAGGGGTGGCGCAGCCGAGCATGGAAGAGGGCTCTGGACAGCTCCTGGAAGGG
  CACGTGCACATGGCTGCAGTGGACCTCCTGATCGGTGGCACTGAGACCACAGCAAACACCCTCTCCTGGG
  CCGTGGTTTTTTTGCTTCACCACCCTGAGATTCAGCAGCGACTGCAGGAGGAGCTAGACCACGAACTGGG
  CCCTGGTGCCTCCAGCTCCCGGGTCCCCTACAAGGACCGTGCACGGCTGCCCTTGCTCAATGCCACCATC
  GCCGAGGTGCTGCGCCTGCGGCCCGTTGTGCCCTTAGCCTTGCCCCACCGCACCACACGGCCCAGCAGCA
  TCTCCGGCTACGACATCCCTGAGGGCACAGTCATCATTCCGAACCTCCAAGGCGCCCACCTGGATGAGAC
  GGTCTGGGAGAGGCCACATGAGTTCTGGCCTGATCGCTTCCTGGAGCCAGGCAAGAACTCCAGAGCTCTG
  GCCTTCGGCTGCGGTGCCCGCGTGTGCCTGGGCGAGCCGCTGGCGCGCCTGGAGCTCTTCGTGGTGCTGA
  CCCGACTGCTGCAGGCCTTCACGCTGCTGCCCTCCGGGGACGCCCTGCCCTCCCTGCAGCCCCTGCCCCA
  CTGCAGTGTCATCCTCAAGATGCAGCCTTTCCAAGTGCGGCTGCAGCCCCGGGGGATGGGGGCCCACAGC
  CCGGGCCAGAGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216416 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLLGLLLLLPLLAGARLLWNWWKLQSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVV
  VLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSRNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVV
  EQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIV
  DVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEG
  HVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATI
  AEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRAL
  AFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHS
  PGQSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_000500
• ORF Size: 1485 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_000500.5
• RefSeq Size: 2131 bp
• RefSeq ORF: 1488 bp
• Locus ID: 1589
• UniProt ID: P08686
• Cytogenetics: 6p21.33
• MW: 56 kDa