Human GRK1 (NM_002929) AAV Particle

CAT#: RC214569A1V

AAV ORF Particles, serotype AAV-2, GRK1 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 1 (GRK1), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: GRK1
• Synonyms: GPRK1; RHOK; RK
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214569 representing NM_002929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCGGGTCTTTGGAGACCGTGGTGGCCAACTCTGCCTTCATCGCCGCCCGAGGCAGCTTTGACG
  GCAGCAGCTCCCAACCCTCCCGGGACAAGAAGTACCTGGCCAAGCTCAAGCTGCCCCCGCTGTCCAAGTG
  TGAGTCCCTCCGCGACAGCCTCAGCCTGGAGTTTGAGAGTGTGTGCTTGGAGCAGCCCATCGGCAAGAAG
  CTCTTTCAGCAGTTCCTACAATCGGCAGAGAAGCACCTGCCGGCCCTGGAGCTCTGGAAAGACATCGAGG
  ACTATGACACGGCAGACAATGACCTCCAGCCACAGAAGGCCCAGACCATCCTGGCCCAGTACCTGGACCC
  CCAGGCCAAACTCTTCTGCAGCTTCCTGGATGAGGGGATAGTGGCGAAGTTTAAGGAGGGGCCTGTGGAG
  ATCCAGGACGGGCTCTTCCAGCCCCTGCTGCAGGCCACCCTGGCACACCTGGGCCAAGCCCCCTTCCAGG
  AGTACCTGGGCAGCCTGTACTTCCTGAGGTTCCTGCAGTGGAAGTGGCTGGAAGCCCAGCCCATGGGGGA
  GGACTGGTTCCTGGACTTCAGGGTCCTGGGGAAAGGGGGCTTCGGGGAGGTGTCGGCCTGCCAGATGAAG
  GCGACCGGCAAGCTGTATGCCTGCAAGAAGCTGAACAAGAAGCGGCTGAAGAAGAGGAAGGGCTACCAGG
  GTGCTATGGTGGAGAAGAAGATTCTGATGAAAGTACACAGCAGGTTCATCGTGTCTCTGGCCTATGCGTT
  TGAAACCAAAGCCGACCTCTGTCTGGTGATGACCATCATGAACGGAGGTGACATCAGGTACCACATCTAC
  AACGTGAATGAGGAGAACCCTGGCTTCCCGGAGCCGCGCGCCCTCTTCTACACGGCGCAGATCATCTGCG
  GCCTGGAGCACCTGCACCAGAGGCGGATCGTCTACCGCGACCTCAAGCCCGAGAACGTGCTGCTGGACAA
  TGACGGCAATGTCCGGATCTCTGACCTTGGGCTGGCCGTGGAGCTGCTGGACGGACAGAGCAAGACCAAG
  GGCTACGCAGGGACCCCAGGTTTCATGGCCCCCGAGCTCCTGCAGGGCGAGGAGTACGACTTCTCCGTGG
  ACTACTTTGCCCTGGGGGTCACCCTGTATGAGATGATTGCGGCCAGAGGACCCTTCCGAGCCCGTGGAGA
  GAAGGTGGAGAACAAGGAGCTGAAGCACCGGATCATCTCAGAGCCCGTGAAGTACCCTGATAAGTTCAGC
  CAGGCCAGCAAGGACTTCTGCGAGGCGCTGCTGGAGAAGGACCCGGAGAAGCGCCTGGGGTTCAGAGATG
  AGACCTGCGACAAGCTCCGTGCCCACCCCCTCTTCAAGGACCTTAACTGGAGGCAGCTGGAGGCTGGGAT
  GCTGATGCCCCCTTTCATCCCAGACTCCAAAACTGTCTACGCAAAGGATATTCAGGACGTGGGTGCCTTT
  TCCACCGTCAAAGGTGTGGCCTTTGACAAAACAGACACAGAATTCTTTCAGGAATTTGCCACTGGCAACT
  GCCCCATCCCCTGGCAGGAGGAGATGATCGAGACGGGCATCTTTGGCGAGCTGAACGTGTGGCGCTCGGA
  CGGTCAGATGCCGGACGACATGAAGGGCATCTCCGGGGGCTCCAGCTCCTCGTCCAAGTCAGGGATGTGT
  CTGGTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214569 representing NM_002929
  Red=Cloning site Green=Tags(s)
  
  MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKK
  LFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVE
  IQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMK
  ATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIY
  NVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTK
  GYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFS
  QASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAF
  STVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMC
  LVS*
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_002929
• ORF Size: 1689 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_002929.2, NP_002920.1
• RefSeq Size: 2100 bp
• RefSeq ORF: 1692 bp
• Locus ID: 6011
• UniProt ID: Q15835
• Cytogenetics: 13q34
• MW: 63.3 kDa