Mouse Hsd3b1 (NM_008293) AAV Particle

CAT#: MR205766A1V

AAV ORF Particles, serotype AAV-2, Hsd3b1 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Hsd3b1
• Synonyms: 3-beta-HSD I; D3Ertd383e
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205766 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCAAGATGTTGG
  TGCAGGAGAAAGAACTGCAGGAGGTCAGAGCTCTGGACAAAGTATTCCGACCAGAAACCAAGGAGGAATT
  CTCCAAGCTGCAGACAAAGACCAAGGTGACAGTGTTGGAAGGAGACATTCTGGATGCCCAGTGCCTGAGG
  AGAGCCTGCCAGGGCATCTCTGTTGTCATCCACACTGCTGCTGTCATTGATGTCACAGGTGTCATTCCCA
  GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACCTATTGGAGGCCTGTGTTCAAGCAAGTGT
  GCCAGCCTTCATCTTCTGCAGCTCAGTTGATGCTGCAGGGCCCAACTCGTACAAGAAGATCGTCCTGAAT
  GGCCACGAGGAACAGAATCATGAAAGCACATGGTCTGATCCATACCCATACAGCAAAAAGATGGCTGAGA
  AGGCAGTACTGGCAGCCAATGGGAGCATGCTGAAAAATGGTGGCACTTTGAATACTTGTGCCTTAAGACC
  CATGTACATTTATGGGGAGAGAAGTCCATTCATTTTTAATGCAATAATTAGGGCCCTCAAAAATAAGGGT
  ATTCTGTGTGTTACTGGCAAATTCTCCATAGCCAACCCAGTATATGTGGAAAATGTGGCCTGGGCACACA
  TTCTGGCAGCCAGGGGCCTTCGAGACCCCAAGAAGTCTACAAGCATCCAAGGACAGTTCTACTACATCTC
  AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTACACCCTGAGTAAGGAATGGGGGCTCCGCCCT
  AATGCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC
  TGCTACGTCCAGTCTACAGGTATAGACCTCTCTTTAACCGCCACTCGATCACACTGTCAAATAGCACGTT
  CACTTTTTCTTACAAGAAAGCTCAGCGAGATCTGGGCTATGAGCCACTTGTCAACTGGGAGGAAGCAAAG
  CAGAAAACCTCAGAGTGGATAGGGACAATAGTGGAACAGCACAGGGAGATATTGGACACAAAGTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205766 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLR
  RACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDAAGPNSYKKIVLN
  GHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKG
  ILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRP
  NASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHSITLSNSTFTFSYKKAQRDLGYEPLVNWEEAK
  QKTSEWIGTIVEQHREILDTKCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_008293
• ORF Size: 1122 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_008293.2, NP_032319.1
• RefSeq Size: 1852 bp
• RefSeq ORF: 1122 bp
• Locus ID: 15492
• UniProt ID: P24815
• Cytogenetics: 3 42.89 cM
• MW: 42 kDa