ACAT1 (ACACA) (NM_198834) Human 3' UTR Clone

CAT#: SC218496

3' UTR clone of acetyl-Coenzyme A carboxylase alpha (ACACA) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACACA
• Synonyms: ACAC; ACACAD; ACC; ACC1; ACCA
• ACCN: NM_198834
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC218496 3’UTR clone of NM_198834
  The sequence shown below is from the reference sequence of NM_198834. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTCTCCACAATGGATTCCCCTTCCACGTAGGAAGAGCTTCCTGCCTGTCCCTGCCCTGTCTCTGGAGAA
  AAGGGCTAGAGCTGCCTTTTACAACTGTAACCACTGTAATGAGAAGGCACAGGAGACCCAGCACTGGAG
  TCAAATGGCATTTTACTTCCTCTCGTTTCAGGTTATGCATGACATCCTGGGATGTAAGATCACAGAATC
  CCCCTCCAGCCCACCAGTCACACCTACCCCATTCAGTATTTATTACCCTGGCCAGGCCTAGTCCTCCAC
  TCCCTGCACAGGACTGAGAAGGCAATGAAAGGTACAAACATGTACCATGAGGTCTTACTAACCAAAGTA
  GGGCTGCCCCTCCTGTCCTGACAGCCCCTTGGCCTCCCAGCATGGGGAAGCGTGAGGAGTTGCCCAGCA
  GTGAGCAGCCCCCCTCACTCCTGGCCCCATGAGCCGCAGCCACAGGCAGCAGAGGAGGGCTAAGGAGAG
  GAGGAAGCCTCAAGTCCATTGTTTATTACCCCGACTCTTAGCCCAGCACACAGTAGGCACTGGAGAGGA
  ATGATTCCCAGTTTAACCACACTACGGTACCTTTTATGAAGAAAAATTAGAGCATAAAATCTACTACAA
  GCTCCATAGGAACTCAAAGATGAGGGCAAAACTGTGAGCCAAGAAGCAGAGAAAGAAAATAGAACCAGT
  TATTCTTGATTTAGGGGACCTCAACCTTGGGTTCAGTCTCTGAGGACAAAGGGAAAGGTAGTTGTTGGC
  CTGCCTCTCGCCTGCACGTCACTGCTGGACTAGCTGTCGCATGTGGCTGGGAGCTGCAAGGCCAGTGCT
  TGAGGGGCCCCAGCAGTTCCACAGGTGGTGAAGCCTGAGTTGGCAGAGGAGGAGCCAGAAGAGAACTGC
  CCTTTCTGCACTGGTGGAAACTAGTTATTTATGCCATGTGGAGAGCCAGTGAGATAGATAGATAGTCTG
  TTTGTTTTGAGGACTTGGAAAGTTGTTCCTATGAAGCCTGGAGCTTGGATGGTTTTGAGAGGTTAATGG
  TGCCTCCACACTCACTCTTCCCTAGTTCCAGGATTACTGTCCTAGCAGCTAACGGTTCTACTCTCTTCC
  CCAGAGTGTAGACAGGCAGCAGGTCTCCCCACAGCTCTGAAAGGACCCTGGTGACAGCTACACCCTCAG
  CACCAGGAGCTGGCCTTCCTGATGAGGGAGGCTTCCAGGAAACACAGAATCCACATGACCTTAAGATTA
  TTTACAACTCAGTCATGGTGCTGCTGTCCTCCAGGCTTACTGGCCCCTCCTGACTGGCATCAGGGGCTT
  CCTCAGGTGGTGGAGAGAGTTTACTTTCAACAACTAGTTTATTCAAGAAAAGAACTTACTGATTCCTCT
  GTTCCTAAAGCAAGAGTGGCAGGTGATCAGGGCTGGTGTAGCATCCGGTTCCTTTAGTGCAGCTAACTG
  CATTTGTCACTGATGACCAAGGAGGAAATCACTAAGACATTTGAGAAGCAGTGGTATGAACGTTCTTGG
  ACAAGCCACAGTTCTGAGCCTTAACCCTGTAGTTTGCACACAAGAACGAGCTCCACCTCCCCTTCTTCA
  GGAGGAATCTGTGCGGATAGATTGGCTGGACTTTTCAATGGTTCTGGGTTGCAGGTGGGCACTGTATGG
  CTGGGTATGGAGCGGACAGCCCCCAGGAGTCAGAGCCTCAGCCCGGCTGCCCTGGTGGAAGGTACAGGT
  GTTCAGCACCTTCAGAAAAGGGCATAAAGTGGTGGGGGACAATTCTCAGTCCAGGAAAATGCATTGACC
  ATTGCTGGCTATTTGCTTACCTAGTAAGAATTGGATTCATTTTTGACCAGATTATTCTTCTATGCTTTT
  TTGCAATAAATCAAATCCCACATATCTACAAGTGGTATGAAGTCCTGCACCCCCCAGGAGGCCTGTCCA
  GGCATGTCTTCAGAGGCAGGGTGGGTTACACTCATTTACCTCCCCTCTCCCCACCAAATTATGACACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_198834.3
• Summary: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 31
• MW: 73.2