Amyloid Precursor Protein (APP) (NM_201414) Human 3' UTR Clone

CAT#: SC212695

3' UTR clone of amyloid beta (A4) precursor protein (APP) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Amyloid Precursor Protein
• Synonyms: AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI; CTFgamma; CVAP; PN-II; PN2; preA4
• ACCN: NM_201414
• Insert Size: 1150 bp
• Sequence Data:
  Insert Sequence

  >SC212695 3’UTR clone of NM_201414
  The sequence shown below is from the reference sequence of NM_201414. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAAC
  CATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATT
  TACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACAC
  ATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTAC
  TGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCC
  CTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGA
  ATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTG
  ATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATG
  ACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACG
  TTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTC
  CACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTG
  GGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTA
  CAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCC
  CGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAA
  GAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAA
  GTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTG
  TATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_201414.3
• Summary: This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]
• Locus ID: 351
• MW: 44.5