Caspase 9 (CASP9) (NM_001229) Human 3' UTR Clone

CAT#: SC208909

3' UTR clone of caspase 9 apoptosis-related cysteine peptidase (CASP9) transcript variant alpha for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Caspase 9
• Synonyms: APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
• ACCN: NM_001229
• Insert Size: 1572 bp
• Sequence Data:
  Insert Sequence

  >SC208909 3’UTR clone of NM_001229
  The sequence shown below is from the reference sequence of NM_001229. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGGAAAAAACTTTTCTTTAAAACATCATAAGGCCAGGGCCCCTCACCCTGCCTTATCTTGCACCCCAAA
  GCTTTCCTGCCCCAGGCCTGAAAGAGGCTGAGGCCTGGACTTTCCTGCAACTCAAGGACTTTGCAGCCG
  GCACAGGGTCTGCTCTTTCTCTGCCAGTGACAGACAGGCTCTTAGCAGCTTCCAGATTGACGACAAGTG
  CTGAACAGTGGAGGAAGAGGGACAGATGAATGCCGTGGATTGCACGTGGCCTCTTGAGCAGTGGCTGGT
  CCAGGGCTAGTGACTTGTGTCCCATGATCCCTGTGTTGTCTCTAGAGCAGGGATTAACCTCTGCACTAC
  TGACATGTGGGGCCAGGTCACCCTTTGCTGTGAGGCTGTCCTGTACATTGTGGGATGTTCAGCACTGTC
  CCTTGCCTCAATGCCAGTAACGCGTCTTCCTGAGTGGTGCCAAACAAAAAGGTTCTCAGGTGTTGCCAA
  ATATGTCCTGGGGTATAAAACTTTCCTCGCCTGACAACCACTGGTCTGTAGGGATTTTTGGCTACACAC
  AAACCAGTATCGCTCATAGATCAGCAAACCGGGGCCTACTAGAGTCTGAACAGCTGTAATCTATGAATT
  CTAAGTGAAATTTTAAAAATTGTTAATTTTTCCTATATTGCATTAATTTTAAAAAATAAATCTGAGGCA
  AATATGGACTCTCTTTTGCCTATTTCTTCCCTCATTTTGCTCCAACTCTTTCTTCTTCCTTACAAAAGA
  GACTTTTGCTTTTTTCGAAACATTTCCCCATGTTTTTCTGGGGTCTCGCTATGTTGCCCAGGCTGGTCT
  CAAACTCCTGGGCTCAAGTGACCCTCCCAAGTAGCTCTTACTACAGGCGTGCACCATTGCACCCAGCCC
  CATTTATTCATGTCTTATTTCACTTGATCCTTATCCCATCCCAGGAAGGCAACAAGGGTGAGAACCCTG
  TGCTCAGGGAGGTTAGGTCTCTTGTCCAAGGGAAAACGATTATCCAGAGAAGAGACCTGGCCAGAACCT
  GGGTCCCCTGAGTCCTAGCCATGCTTCCCATGTGCCTTACTTGCTGAAGCACCCCCGGACTGCAGTGTG
  AACGTGCTGTGCAATAGTGACACGCTGGGCTTCCCCACAAGGCTCCACCCTGAGGTCTTTTAAGCTGTC
  CTTATGCCAGCCTATTTCTTGTTTTTTGGGCCTTTTTTTTTGGAGATAGGGTCTCACTCTGTCGCCCAG
  GCTGGAGTGCAATGACGCAATCTTGGCTTATTGCAGTCTCGACCTCCTGGGCTCAAGAGATCCTTCCAC
  CTCAGCCACCTGAGTAGCTTGGACTACAGGTGTGCACCACCTCTCCCAGTTAATTTTTGTATTTTTAGT
  AGAGACAGAGTTATGCCATGTTACTCAGGCTGGTCTTGAACTCCTGGACTCAAGCGATCAGCCTGCCTT
  AGCCTCCCAAAGTGCAGGGGTTACAGGCTTGAGCCATTGCGCCTGACCTATTTCTGGTTCTTAGGGCCC
  TGGATGTTAGGATGGATTTCTGAATTAATAATAATAATAAAACCCTCATCAAGA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001229.5
• Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
• Locus ID: 842
• MW: 57.7