ATF6 (NM_007348) Human 3' UTR Clone

CAT#: SC205479

3' UTR clone of activating transcription factor 6 (ATF6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATF6
• Synonyms: ACHM7; ATF6A
• ACCN: NM_007348
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC205479 3’UTR clone of NM_007348
  The sequence shown below is from the reference sequence of NM_007348. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTCAGCACCATCCCTGAGTCATTACAATAGCACCCTGCAGCTATGCTGGAAAACTGAGCGTGGGACCCT
  GCCAGACTGAAGAGCAGGTGAGCAAAATGCTGCTTTCTGCCTTGGTGGCAGGCAGAGAACTGTCTCGTA
  CTAGAATTCAAGGAGGAAAGAAGAAGAAATAAAAGAAGCTGCTCCATTTTTCATCATCTACCCATCTAT
  TTGGAAAGCACTGGAATTCAGATGCAAGAGAACAATGTTTCTTCAGTGGCAAATGTAGCCCTGCATCCT
  CCAGTGTTACCTGGTGTAGATTTTTTTTTCTGTACCTTTCTAAACCTCTCTTCCCTCTGTGATGGTTTT
  GTGTTTAAACAGTCATCTTCTTTTAAATAATATCCACCTCTCCTTTTTGCCATTTCACTTATTGATTCA
  TAAAGTGAATTTTATTTAAAGCTATGCCACACATGCATGTTCAAATGGTTTCCACTGATTCGATTTTTC
  ATTCATTTAATGCAAACCCATTCTGGATATTGTGCTTATTTGAGAAAACACATTTCAAAACCAGAAAAG
  CCAAAAACACTCCAAAAACAAGCAAAACAATTTGGAGCTTTAGATAAAAGGAAAAACTCCCAGTTGGTA
  AAGTTTATCTTTACTTAGGATTTGTGGCTCACACCTAAACAAAGGGGGTCAGGGAGTGGGTACAAATTT
  GAGAAAATAGAAGGGTAAGGGAAGGGCCAGTGGTGGGGTTTGGAGAGAGGAGATAGCTCCATTAATACA
  CATGTTTAAAAGATGGAAAGTTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCAC
  GAGGTCAGGAGATCAAGACCATCCCGGCTAAAACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAT
  TAGCCGGGCGTAGTGACGGGCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGA
  ACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCCCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAG
  ACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAGATGGAAAGTTCGATGTGACTGCAGTATGAGATT
  AAAGCCACAACTATTGTTTATTTTGGGGACTCTAGGCCACCAAGTATTAGCACACATACTTATGTTTTC
  TCTACTAATCTGGTCCAGGTCCTCATGGACCACAGGACAAAGCTTTCATTTTCATTCATTCTTCTATTG
  AAATTATACCAAATTCAGCTGAGGAATATGGAAGTAACTTTAGACTTAAACAAGACAAAAGTTTTTTCA
  CTGAAGAATTGACAAGTATTTGCTCCTTAAAACAACGCAGATTAGTGAACGTGGATTCCTGCTGAGGGA
  GTGCATCCCATAATATGGCAATAATTTTCAGTTTCTCCAACGAAAAGATAGTGAAGGAATTAAATCTTT
  TGTCCTCCCATGGTTAAAAAAAAAAAAAAAGCTGTGTTCATTTTTACTGTACTATGCCTCTTTTTTCAC
  CATAGTAGACAATTATGTTTCATTTGATGAATTCATAGAACTGGATCTCATACAGCGATGTCCTCTCTA
  ATGTTCTACCTTTCAGTTTCTAAAGTGAGTCTTCCTCCCTCTCCTACAAAACTTTTCAATTTTTTGATG
  TAACTCATCTACAAATACTGTTTCTTACCCCAGTTGACTTGCCTTTGTCAGATTTCTTCTTGTTCCACA
  CTATAGCAATCAATTTCTCTTCTTCCTTACAAGAAAGGGAACGAGAAATTGTAGCAACCTCTCAAGGAT
  TATATGCAGCTAGTTAGTTTTCTGCCTGTGAAATTAGGTCTGGCTCCTAAATAATTTTAAAGAACCATC
  AGCACTTCTAACTCTCTGGACAGGTGCCTCTTTGTCCAAGCTAGTTAAATGCTTTCCAAGGAAATCAGT
  TCAACTTTTGTGAGCGGGGAAAAGCAGGGCTTTATTGTTGTGTTACCTGGGAGTCTGGAGTTTGAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_007348.4
• Summary: This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
• Locus ID: 22926
• MW: 76.3