TMS1 (PYCARD) Rabbit Polyclonal Antibody

CAT#: TA380583

ASC / TMS1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ASC / TMS1 Rabbit pAb (TA380583) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of C6 cells using ASC / TMS1 Rabbit pAb (TA380583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using ASC / TMS1 Rabbit pAb (TA380583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using ASC / TMS1 Rabbit pAb (TA380583) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of Rat lung using 3ug ASC/TMS1 antibody (TA380583). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (TA380583) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ASC / TMS1
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 15kDa/19kDa/21kDa
• Gene Name: PYD and CARD domain containing
• Database Link:
  Entrez Gene 29108 Human
  UniProt ID Q9ULZ3
• Background: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ASC; CARD5; hASC; MGC10332; TMS; TMS-1; TMS1