GLUD1 Rabbit Polyclonal Antibody

CAT#: TA376611

GLUD1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using GLUD1 antibody (TA376611) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of C6 cells using GLUD1 antibody (TA376611) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using GLUD1 antibody (TA376611) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of C6 cells using GLUD1 antibody (TA376611) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug GLUD1 antibody (TA376611). Western blot was performed from the immunoprecipitate using GLUD1 antibody (TA376611) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:100 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 54-240 of human GLUD1 (NP_005262.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa/46kDa/61kDa
• Gene Name: glutamate dehydrogenase 1
• Database Link:
  Entrez Gene 2746 Human
  UniProt ID P00367
• Background: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
• Synonyms: GDH; GDH1; GLUD; MGC132003