PAR2 (F2RL1) Rabbit Polyclonal Antibody

CAT#: TA376060

PAR2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PAR2 antibody (TA376060) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of HepG2 cells using PAR2 Rabbit pAb (TA376060) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using PAR2 Rabbit pAb (TA376060) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:200 - 1:1000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 318-397 of human PAR2 (NP_005233.3).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa
• Gene Name: F2R like trypsin receptor 1
• Database Link:
  Entrez Gene 2150 Human
  UniProt ID P55085
• Background: Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence.
• Synonyms: GPR11; PAR-2; PAR2