Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

CAT#: TA374365

Cyclin B1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (TA374365) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of HeLa cells using Cyclin B1 Rabbit pAb (TA374365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb (TA374365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb (TA374365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (TA374365). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (TA374365) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa/48kDa
• Gene Name: cyclin B1
• Database Link:
  Entrez Gene 891 Human
  UniProt ID P14635
• Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
• Synonyms: CCNB