HLA DMB (HLA-DMB) Rabbit Polyclonal Antibody

CAT#: TA365757

HLA-DMB rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: K562 and Jurkat cell lysates Primary antibody: TA365757 (HLA-DMB Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

• 

  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA365757 (HLA-DMB Antibody) at dilution 1/40 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA365757 (HLA-DMB Antibody) at dilution 1/40, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: K562 and Jurkat cell lysates
  IHC: 30-150
  Positive control: Human prostate cancer
  Predicted cell location: Cell membrane
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human HLA-DMB
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 29 kDa
• Gene Name: major histocompatibility complex, class II, DM beta
• Database Link:
  Entrez Gene 3109 Human
  UniProt ID P28068
• Background: HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
• Synonyms: D6S221E; RING7